Cloning and expression of the VHDL receptor from fat body of the corn ear worm, Helicoverpa zea

In Noctuids, storage proteins are taken up into fat body by receptor-mediated endocytosis. These include arylphorin and a second, structurally unrelated very high-density lipoprotein (VHDL). Previously, we have isolated a single storage protein receptor from the corn earworm, Helicoverpa zea, which binds both VHDL and arylphorin. The receptor protein is a basic, N-terminally blocked, ∼80 kDa protein that is associated with fat body membranes. Microsequencing of proteolytic fragments of the isolated receptor protein revealed internal sequences that were used to clone the complete cDNA of the VHDL receptor by 3′ and 5′ RACE techniques. The receptor protein, when expressed in vitro via a suitable insect expression vector, reacted with antibodies against the native VHDL receptor and bound strongly to its ligand VHDL, thus confirming that the cloned cDNA represents indeed the previously purified VHDL receptor. The receptor protein and a second, similar protein also found associated with the fat body membrane show considerable homology to putative basic juvenile hormone suppressible proteins cloned previously from other Noctuid species. Sequence analysis revealed that the receptor is likely a peripheral membrane protein that may mediate the selective uptake of VHDL. Abbreviation: / BJHSP: basic juvenile hormone suppressible protein FITC: fluorescein isothiocyanate HRP: horseraddish peroxidase RACE: rapid amplification of cDNA ends VHDL: very high density lipoprotei

LongSAGE profiling of nine human embryonic stem cell lines

Analysis of a 2.6 million longSAGE sequence tag resource generated from nine human embryonic stem cell lines reveals an enrichment of RNA binding proteins and novel ES-specific transcripts

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insectscience.org Cloning and expression of the VHDL receptor from fat body of the corn ear worm, Helicoverpa ze

Purification of the VHDL receptor Panel A: Affinity-purified VHDL receptor (1 μg) appeared homogenous in a 12 % SDS gel

<p><b>Copyright information:</b></p><p>Taken from "Cloning and expression of the VHDL receptor from fat body of the corn ear worm, "</p><p>Journal of Insect Science 2004;4():-.</p><p>Published online 27 Feb 2004</p><p>PMCID:PMC455680.</p><p>Copyright © 2004. Open access; copyright is maintained by the authors.</p> The ligand blot was carried out with FITC-labeled VHDL, and the Western blot with anti-VHDL-receptor antiserum, as described in Materials and Methods. Panel B: The affinity-purified fraction could be further separated into two proteins on a larger 15 % SDS gel: the 80 kDa band is the receptor and less prominent 78 kDa protein. Molecular weight markers (175, 83, 62, 48, 33 kDa) are shown on the far right lane

Intelligent Guided E-Learning Systems for Early Learners with Autism Spectrum Disorder

There is a burgeoning need to consider new ways of providing early educational services for young and often newly diagnosed children with Autism Spectrum Disorder (ASD) and their families. Such children do not respond naturally to linear curricular delivery, normally utilized in inclusive classrooms that predominate public education, but rather need an educational model incorporating intra and interpersonal development skills. In addition, there is an urgent need for the ability of keeping track of and addressing uneven progress in specific areas; characteristic of learners with ASD. It is suggested that a new curricular model be designed that integrates the advantages of e-learning for data management and communication exchange with the inclusion classroom learning. A multi-disciplinary approach to the problem has lead to the proposal of an alternate model using an Intelligent Guided E-Learning System, which can be of benefit to such learners, their parents, and their teachers. This system utilizes a Knowledge Representation model that incorporates the complex multidisciplinary data related with ASD, along with curricular information as well as other Artificial Intelligence techniques that guide the curriculum in a simple and directed, yet evolving, manner such that the complexity increases as the learner with ASD's understanding progresses

Differentially expressed isoforms (as predicted by LongSAGE tag positions) for the transcript (see text)

The tag sequence at position 9 results in the loss of the 3' UTR region targeted by evolutionarily conserved miRNAs. Putative miRNA target sites were predicted using miRanda [34] and are represented by hashed boxes.<p><b>Copyright information:</b></p><p>Taken from "LongSAGE profiling of nine human embryonic stem cell lines"</p><p>http://genomebiology.com/2007/8/6/R113</p><p>Genome Biology 2007;8(6):R113-R113.</p><p>Published online 14 Jun 2007</p><p>PMCID:PMC2394759.</p><p></p