SHIMM as an atmospheric profiler on the Nickel Telescope

Optimal atmospheric conditions are beneficial for detecting exoplanets via high contrast imaging (HCI), as speckles from adaptive optics' (AO's) residuals can make it difficult to identify exoplanets. While AO systems greatly improve our image quality, having access to real-time estimates of atmospheric conditions could also help astronomers use their telescope time more efficiently in the search for exoplanets as well as aid in the data reduction process. The Shack-Hartmann Imaging Motion Monitor (SHIMM) is an atmospheric profiler that utilizes a Shack-Hartmann wavefront sensor to create spot images of a single star in order to reconstruct important atmospheric parameters such as the Fried parameter ($r_0$), $C_n^2$ profile and coherence time. Due to its simplicity, the SHIMM can be directly used on a telescope to get in situ measurements while observing. We present our implementation of the Nickel-SHIMM design for the one meter Nickel Telescope at Lick Observatory. We utilize an HCIPy simulation of turbulence propagating across a telescope aperture to verify the SHIMM data reduction pipeline as we begin on-sky testing. We also used on-sky data from the AO system on the Shane Telescope to further validate our analysis, finding that both our simulation and data reduction pipeline are consistent with previously determined results for the Fried parameter at the Lick Observatory. Finally, we present first light results from commissioning of the Nickel-SHIMM.Comment: Conference Proceedings for 2023 SPIE Optics and Photonics, Techniques and Instrumentation for Detection of Exoplanets X

Fluorescent Mimics of Cholesterol that Rapidly Bind Surfaces of Living Mammalian Cells

Mammalian cells acquire cholesterol, a critical membrane constituent, through multiple mechanisms. We synthesized mimics of cholesterol, fluorescent N-alkyl-3β-cholesterylamine-glutamic acids, that are rapidly incorporated into cellular plasma membranes compared with analogous cholesteryl amides, ethers, esters, carbamates, and a sitosterol analogue. This process was inhibited by ezetimibe, indicating a receptor-mediated uptake pathway

The Impact of Shared Versus Individual Office Space on Therapist Appraisal of Their Work Environment

Objective:  The primary objective was to investigate the impact of shared versus individual office space on therapist appraisal of the work physical and social environment, and overall appraisal of working conditions. Method:  Therapists (n = 59) from Child and Adolescent Mental Health Services in Western Australia were surveyed about their appraisal of the social and physical work environment, in addition to their overall appraisal of working conditions. Results:  Compared to therapists with individual offices, therapists occupying shared office space reported lower appraisal of the work physical environment and lower overall appraisal of working conditions. No difference was found between groups for appraisal of the work social environment. Additionally, when statistically controlling for office space, both the appraisal of the social and physical environment made an independent contribution to the prediction of overall work satisfaction. Conclusion:  This research reveals that shared office space can negatively impact therapist appraisal of their work environment and reduce overall appraisal of working conditions. Additionally, results reveal the high importance of the physical environment for staff satisfaction in a mental health service

The importance of the Physical Environment for Child and Adolescent Mental Health Services

Objective: This study examined the relationships between appraisals of the physical environment with the subjective experience of consumers, and work satisfaction of clinicians, in Child and Adolescent Mental Health Services (CAMHS). Design, setting, and outcome measures: A survey of clinicians, parent/guardians, and child/adolescents was conducted across eight community CAMHS in Western Australia. Respondents evaluated the waiting room and therapy rooms on a number of environmental attributes, and factor analysis was carried out to confirm that these ratings loaded on an overall appraisal of the physical environment measure. This measure was thencorrelated with self-reported subjective experience of consumers, and overall work satisfaction of staff members. Results: Clinicians were found to be much more critical of the physical environment compared with consumers. Moderate associations were found between appraisal of the physical environment and subjective experience of consumers. A strong positive association was found between clinician appraisal of the physical environment and overall work satisfaction. Conclusions: The present study adds to the limited existing research arguing for the important role that the physical environment can have upon both consumer and staff experience in mental health settings. The present study provides empirical evidence to justify steps being taken to enhance the physical environment in mental health clinics. The inter-relationship between physical environment attributes suggests there is potential for managers to improve the overall perception of clinic space via relatively small actions (e.g., adding a nice piece of artwork). Abbreviations: CAMHS – Child and Adolescent Mental Health Services

Investigating changes in blood-cerebrospinal fluid barrier function in a rat model of chronic hypertension using non-invasive magnetic resonance imaging

Chronic hypertension is a major risk factor for the development of neurodegenerative disease, yet the etiology of hypertension-driven neurodegeneration remains poorly understood. Forming a unique interface between the systemic circulation and the brain, the blood-cerebrospinal fluid barrier (BCSFB) at the choroid plexus (CP) has been proposed as a key site of vulnerability to hypertension that may initiate downstream neurodegenerative processes. However, our ability to understand BCSFB’s role in pathological processes has, to date, been restricted by a lack of non-invasive functional measurement techniques. In this work, we apply a novel Blood-Cerebrospinal Fluid Barrier Arterial Spin Labeling (BCSFB-ASL) Magnetic resonance imaging (MRI) approach with the aim of detecting possible derangement of BCSFB function in the Spontaneous Hypertensive Rat (SHR) model using a non-invasive, translational technique. SHRs displayed a 36% reduction in BCSFB-mediated labeled arterial water delivery into ventricular cerebrospinal fluid (CSF), relative to normotensive controls, indicative of down-regulated choroid plexus function. This was concomitant with additional changes in brain fluid biomarkers, namely ventriculomegaly and changes in CSF composition, as measured by T1 lengthening. However, cortical cerebral blood flow (CBF) measurements, an imaging biomarker of cerebrovascular health, revealed no measurable change between the groups. Here, we provide the first demonstration of BCSFB-ASL in the rat brain, enabling non-invasive assessment of BCSFB function in healthy and hypertensive rats. Our data highlights the potential for BCSFB-ASL to serve as a sensitive early biomarker for hypertension-driven neurodegeneration, in addition to investigating the mechanisms relating hypertension to neurodegenerative outcomes

Investigating the beneficial traits of Trichoderma hamatum GD12 for sustainable agriculture : insights from genomics

Trichoderma hamatum strain GD12 is unique in that it can promote plant growth, activate biocontrol against pre- and post-emergence soil pathogens and can induce systemic resistance to foliar pathogens. This study extends previous work in lettuce to demonstrate that GD12 can confer beneficial agronomic traits to other plants, providing examples of plant growth promotion in the model dicot, Arabidopsis thaliana and induced foliar resistance to Magnaporthe oryzae in the model monocot rice. We further characterize the lettuce-T. hamatum interaction to show that bran extracts from GD12 and an N-acetyl-β-D-glucosamindase-deficient mutant differentially promote growth in a concentration dependent manner, and these differences correlate with differences in the small molecule secretome. We show that GD12 mycoparasitises a range of isolates of the pre-emergence soil pathogen Sclerotinia sclerotiorum and that this interaction induces a further increase in plant growth promotion above that conferred by GD12. To understand the genetic potential encoded by T. hamatum GD12 and to facilitate its use as a model beneficial organism to study plant growth promotion, induced systemic resistance and mycoparasitism we present de novo genome sequence data. We compare GD12 with other published Trichoderma genomes and show that T. hamatum GD12 contains unique genomic regions with the potential to encode novel bioactive metabolites that may contribute to GD12's agrochemically important traits. Read Full Tex