The immunohistochemical analysis of fibronectin, collagen type III, laminin, and cytokeratin 5 in putrified skin

Fibronectin, collagen type III, laminin, and cytokeratin 5 were visualized in normal skin and in skin showing early or advanced signs of autolytic decomposition to prove whether the immunohistochemical analysis of these antigens can provide useful information for an age-estimation of skin wounds obtained from putrified corpses. In cases with early signs of decomposition (visible course of veins, greenish discoloration) and without microscopic alterations like relaxation of the epidermal cell layers or destruction of the blood vessel structures, the staining pattern was identical to that found in normal, non-putrefied skin. In skin already showing microscopic alteration of the tissue structure, fibronectin and collagen type III could not be localized unambiguously. The distribution of laminin and cytokeratin 5, however, was well preserved. In advanced putrefied skin no reliable staining results could be obtained for fibronectin, collagen type III, and laminin. Even though cytokeratin 5 was still detectable in remnants of decomposition-resistant skin appendages, no information useful for an age-estimation of skin wounds can be obtained due to the autolytic detachment of the epidermal layers

Power and policy in floodplain management, drawing on research in Alberta, Canada

Floodplain management policy implementation and change involves a range of actors and opportunities for power to be exercised. Considering four conceptualizations of power envisioned by Dahl, Bachrach & Baratz, Lukes, and Emerson, this paper explores the actors and instances where power emerges in floodplain management policy implementation and change, using Alberta, Canada, as our focus. We consider which conceptualization is most reflective of the Alberta context. We conclude that all four views of behaviour are relevant here, with some power dynamics between actors more complex than others. Further, these dynamics change over time, and therefore the trade-offs to be made also change. Understanding power relations allows for clearer identification of these trade-offs and, therefore, the potential winners and losers in floodplain management policy. We describe strategies for assisting in this process of managing power relations, including information sharing, transparency and actively managing the policy process

The robustness of flood insurance regimes given changing risk resulting from climate change

The changing risk of flooding associated with climate change presents different challenges for the different flood insurance market models in use around the world, which vary in respect of consumer structure and their risk transfer mechanism. A review of international models has been undertaken against three broad criteria for the functioning and sustainability of a flood insurance scheme: knowing the nature of the insurable risk; the availability of an insurable population; and the presence of a solvent insurer. The solvency of insurance markets appears strong, partly because insurers and reinsurers can choose to exclude markets which would give rise to insolvency or can diversify their portfolios to include offsetting perils. Changing risk may threaten solvency if increasing risk is not recognised and adjusted for but insurability of flood risk may be facilitated by the use of market based and hybrid schemes offering greater diversification and more flexibility. While encouragement of mitigation is in theory boosted by risk based pricing, availability and affordability of insurance may be negatively impacted. This threatens the sustainability of an insurable population, therefore the inclusion of the state in partnership is beneficial in ensuring continuity of cover, addressing equity issues and incentivising mitigation

Assessing water availability under pastoral livestock systems in drought-prone Isiolo District, Kenya

Water availability / Water demand / Surface water / Groundwater / Wells / Salinity / Livestock / Grazing / Land use / Water supply / Drainage / GIS / Databases / Cost recovery

Intensifying agricultural sustainability: an analysis of impacts and drivers in the development of ‘bright spots’

Food security / Farming systems / Sustainable agriculture / Productivity / Investment / Thailand / Palestine / Latin America / Africa

Can a charged ring levitate a neutral, polarizable object? Can Earnshaw's Theorem be extended to such objects?

Stable electrostatic levitation and trapping of a neutral, polarizable object by a charged ring is shown to be theoretically impossible. Earnshaw's Theorem precludes the existence of such a stable, neutral particle trap.Comment: 11 pages, 1 figur

Melanoma-associated adhesion molecule MUC18/MCAM (CD146) and transcriptional regulator Mader in normal human CNS

The proteins MUC18 and Mader have been identified as markers of tumor progression in melanoma cells, MUC18, also known as MCAM (melanoma cell adhesion molecule) and as CD146 (endothelial antigen), is a cell adhesion molecule belonging to the immunoglobulin superfamily, Mader is a transcriptional regulator shown to negatively regulate EGR-1. As it is known that neoplastic cells of neuroectodermal origin frequently express neuron-specific molecules, we studied whether these melanoma-associated antigens are found in normal CNS tissue. We investigated the expression of MUC18/MCAM and Mader in adult human post mortem CNS tissue by immunohistochemistry, immunoblot and two-dimensional gel electrophoresis. Our results show that Mader is preferentially expressed on neurons and glial cells and that the adhesion protein MUC18/MCAM is mainly expressed on vasculature within the CNS. These observations may have important implications for further studies investigating their possible roles in cell adhesion and proliferation control within the CNS

Integrated land and water management for food and environmental security

Water resource management / Food security / Environmental effects / Soil degradation / Water pollution / Watersheds / Urbanization / Public policy / Water quality / Ecosystems / Land resources / Water scarcity / Developing countries / Poverty / Households / Food supply / Economic aspects / Social aspects / Groundwater depletion / Salinity / Wetlands / Investment / Land use / Water use / Training needs assessment / Research priorities