Lamina-associated polypeptide 2 alpha is required for intranuclear MRTF-A activity

Myocardin-related transcription factor A (MRTF-A), a coactivator of serum response factor (SRF), regulates the expression of many cytoskeletal genes in response to cytoplasmic and nuclear actin dynamics. Here we describe a novel mechanism to regulate MRTF-A activity within the nucleus by showing that lamina-associated polypeptide 2 alpha (Lap2 alpha), the nucleoplasmic isoform of Lap2, is a direct binding partner of MRTF-A, and required for the efficient expression of MRTF-A/SRF target genes. Mechanistically, Lap2 alpha is not required for MRTF-A nuclear localization, unlike most other MRTF-A regulators, but is required for efficient recruitment of MRTF-A to its target genes. This regulatory step takes place prior to MRTF-A chromatin binding, because Lap2 alpha neither interacts with, nor specifically influences active histone marks on MRTF-A/SRF target genes. Phenotypically, Lap2 alpha is required for serum-induced cell migration, and deregulated MRTF-A activity may also contribute to muscle and proliferation phenotypes associated with loss of Lap2 alpha. Our studies therefore add another regulatory layer to the control of MRTF-A-SRF-mediated gene expression, and broaden the role of Lap2 alpha in transcriptional regulation.Peer reviewe

RF driven 5G system design for centimeter waves

Abstract 5G system design is a complex process due to a great variety of applications and their diverse requirements. This article describes our experiences in developing a centimeter waves mobile broadband concept satisfying future capacity requirements. The first step in the process was the radio channel measurement campaign and statistical modeling. Then the link level design was performed tightly together with the radio frequency (RF) implementation requirements to allow as large scalability of the air interface as possible. We started the concept development at 10 GHz frequency band and during the project World Radiocommunication Conference 2015 selected somewhat higher frequencies as new candidates for 5G. Thus, the main learning was to gain insight of interdependencies of different phenomena and find feasible combinations of techniques and parameter combinations that might actually work in practice, not only in theory