Bacterial diversity and community in Qula from the Qinghai–Tibetan Plateau in China

Qula is a cheese-like product usually prepared with unpasteurized yak milk under open conditions, with both endogenous and exogenous microorganisms involved in the fermentation process. In the present study, 15 Qula samples were collected from five different regions in China to investigate the diversity of microbial communities using high-throughput sequencing targeting the V3–V4 region of 16S rRNA gene. The bacterial diversity significantly differed among samples of different origins, indicating a possible effect of geography. The result also showed that microbial communities significantly differed in samples of different origin and these differences were greater at the genus than the phylum level. A total of six phyla were identified in the samples, and Firmicutes and Proteobacteria had a relative abundance >20%. A total of 73 bacterial genera were identified in the samples. Two dominant genera (Lactobacillus and Acetobacter) were common to all samples, and a total of 47 operational taxonomic units at different levels significantly differed between samples of different origin. The predicted functional genes of the bacteria present in samples also indicated differences in bacterial communities between the samples of different origin. The network analysis showed that microbial interactions between bacterial communities in Qula were very complex. This study lays a foundation for further investigations into its food ecology

Complete Mitogenome sequencing of the fish louse Argulus japonicus (Crustacea: Branchiura): Comparative analyses and phylogenetic implications

The fish louse Argulus japonicus, a branchiuran crustacean of the Argulidae family, is attracting increasing attention because of its parasitic tendencies and significant health threats to global fish farming. The mitogenomes can yield a foundation for studying epidemiology, genetic diversity, and molecular ecology and therefore may be used to assist in the surveillance and control of A. japonicus. In this study, we sequenced and assembled the complete mitogenome of A. japonicus to shed light on its genetic and evolutionary blueprint. Our investigation indicated that the 15,045-bp circular genome of A. japonicus encodes 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), and 2 ribosomal RNAs (rRNAs) with significant AT and GC skews. Comparative genomics provided an evolutionary scenario for the genetic diversity of 13 PCGs: all were under purifying selection, with cox1 and nad6 having the lowest and highest evolutionary rates, respectively. Genome-wide phylogenetic trees established a close relationship between species of the families Argulidae (Arguloida) and Armilliferidae (Porocephalida) within Crustacea, and further, A. japonicus and Argulus americanus were determined to be more closely related to each other than to others within the family Argulidae. Single PCG-based phylogenies supported nad1 and nad6 as the best genetic markers for evolutionary and phylogenetic studies for branchiuran crustaceans due to their similar phylogenetic topologies with those of genome-based phylogenetic analyses. To sum up, these comprehensive mitogenomic data of A. japonicus and related species refine valuable marker resources and should contribute to molecular diagnostic methods, epidemiological investigations, and ecological studies of the fish ectoparasites in Crustacea

The Gradient Variation of Location Distribution, Cross-Section Area, and Mechanical Properties of Moso Bamboo Vascular Bundles along the Radial Direction

Bamboo is a typical natural fiber-reinforced composite with excellent mechanical properties, which are determined by its special micro-structure. As the reinforcing phase, the vascular bundles play a central role in the control of the mechanical properties of bamboo macro-structure. To find the exact gradient variation of the mechanical properties of these continuously distributed vascular bundles within the bamboo culm, 4-year-old Moso bamboo was chosen to investigate the variation of locate-distribution, cross-section area, and mechanical properties of single vascular bundles along the longitudinal and radial directions with respect to their location from the base, middle, and top sections of bamboo culm, respectively. It shows that the spatial distribution of vascular bundles along the column is distributed exponentially from the inside to the outside of the culm. The cross-section area of the vascular bundles decreased exponentially from the inside to the outside along the radial direction. All the vascular bundles were then carefully separated from bamboo strips and tested via the tensile tests. Test results show that the longitudinal tensile strengths of vascular bundles ranged from 180.44 to 774.10 MPa, and the longitudinal Young’s modulus ranged from 9.00 to 44.76 GPa. The tensile strength of vascular bundles at the outer side was three times higher than that of the inner side, while Young’s modulus at the outer side was three to four times higher than that of the inner side. For all three height positions, the strengths and Young’s modulus of vascular bundles are all exponentially increased from the inner side to the outer side along the radial direction. This work will provide a basis for the highly processed product’s application of bamboo resources and a reference for further study on the trans-scale analysis of the mechanical properties of bamboo

Stereoselective Construction of β-, γ-, and δ-Lactam Rings via Enzymatic C-H Amidation.

Lactam rings are found in many biologically active natural products and pharmaceuticals, including important classes of antibiotics. Methods for the asymmetric synthesis of these molecules are therefore highly desirable, particularly through the selective functionalization of unreactive aliphatic C-H bonds. Here we show the development of a strategy for the asymmetric synthesis of β-, γ-, and δ-lactams via hemoprotein-catalysed intramolecular C-H amidation reaction with readily available dioxazolone reagents. Engineered myoglobin variants serve as excellent biocatalysts for this transformation yielding the desired lactam products in high yields, high enantioselectivity, and on preparative scale. Mechanistic and computational studies elucidate the nature of the C-H amination and enantiodetermining steps and provide insights into protein-mediated control of regioselectivity and stereoselectivity. Additionally, an alkaloid natural product and a drug molecule were synthesized chemoenzymatically in much fewer steps (7-8 vs. 11-12) than previously reported, further demonstrating the power of biosynthetic strategy for the preparation of complex bioactive molecules

Cation Dynamics Governed Thermal Properties of Lead Halide Perovskite Nanowires

Metal halide perovskite (MHP) nanowires such as hybrid organic–inorganic CH₃NH₃PbX₃ (X = Cl, Br, I) have drawn significant attention as promising building blocks for high-performance solar cells, light-emitting devices, and semiconductor lasers. However, the physics of thermal transport in MHP nanowires is still elusive even though it is highly relevant to the device thermal stability and optoelectronic performance. Through combined experimental measurements and theoretical analyses, here we disclose the underlying mechanisms governing thermal transport in three different kinds of lead halide perovskite nanowires (CH₃NH₃PbI₃, CH₃NH₃PbBr₃ and CsPbBr₃). It is shown that the thermal conductivity of CH₃NH₃PbBr₃ nanowires is significantly suppressed as compared to that of CsPbBr₃ nanowires, which is attributed to the cation dynamic disorder. Furthermore, we observed different temperature-dependent thermal conductivities of hybrid perovskites CH₃NH₃PbBr₃ and CH₃NH₃PbI₃, which can be attributed to accelerated cation dynamics in CH₃NH₃PbBr₃ at low temperature and the combined effects of lower phonon group velocity and higher Umklapp scattering rate in CH₃NH₃PbI₃ at high temperature. These data and understanding should shed light on the design of high-performance MHP based thermal and optoelectronic devices

The Complete Mitogenome of <i>Toxocara vitulorum</i>: Novel In-Sights into the Phylogenetics in Toxocaridae

Toxocara vitulorum (Ascaridida: Nematoda) is one of the most common intestinal nematodes of cattle and buffalos and, therefore, represents a serious threat to their populations worldwide. Despite its significance in veterinary health the epidemiology, population genetics, and molecular ecology of this nematode remain poorly understood. The mitogenome can yield a foundation for studying these areas and assist in the surveillance and control of T. vitulorum. Herein, the first whole mitogenome of T. vitulorum was sequenced utilizing Illumina technology and characterized with bioinformatic pipeline analyses. The entire genome of T. vitulorum was 15,045 bp in length and contained 12 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), and two ribosomal RNAs (rRNAs). The gene arrangement (GA) of T. vitulorum was similar to those of other Toxocara species under GA3. The whole genome showed significant levels of AT and GC skew. Comparative mitogenomics including sequence identities, Ka/Ks, and sliding window analysis, indicated a purifying selection of 12 PCGs with cox1 and nad6 having the lowest and highest evolutionary rate, respectively. Whole amino acid sequence-based phylogenetic analysis supported a novel sister-species relationship of T. vitulorum with the congeneric species Toxocara canis, Toxocara cati, and Toxocara malaysiensis in the family Toxocaridae. Further, 12 (PCGs) single gene-based phylogenies suggested that nad4 and nad6 genes shared same topological trees with that of the whole genome, suggesting that these genes were suitable as novel genetic markers for phylogenetic and evolutionary studies of Ascaridida species. This complete mitogenome of T. vitulorum refined phylogenetic relationships in Toxocaridae and provided the resource of markers for population genetics, systematics, and epidemiology of this bovine nematode

Comprehensive Molecular Characterization of the Mitochondrial Genome of the Takin Lungworm <i>Varestrongylus eleguneniensis</i> (Strongylida: Protostrongylidae)

The takin lungworm Varestrongylus eleguneniensis (Strongylida: Protostrongylidae) causes lethal bronchopneumonia and represents severe threats to captive and wild populations. However, until now there has been very limited information available concerning the molecular epidemiology and evolutionary biology of V. eleguneniensis. Mitochondrial genomes (mtDNAs) can provide resources for investigations in these areas and, therefore, can assist with the surveillance and control of this lungworm. Herein, the complete mtDNA of V. eleguneniensis was sequenced and characterized with Illumina pipeline analyses. This circular genome (13,625 bp) encoded twelve protein-coding genes (PCGs), two rRNAs, and twenty-two tRNAs, with notable levels of AT and GC skews. Comparative genomics revealed a purifying selection among PCGs, with cox1 and nad6 having the lowest and the highest evolutionary rate, respectively. Genome-wide phylogenies showed a close relationship between V. eleguneniensis and Protostrongylus rufescens in Strongylida. Single gene (PCGs or rRNAs)-based phylogenies indicated that cox1 and nad5 genes shared the same family-level topology with that inferred from genomic datasets, suggesting that both genes could be suitable genetic markers for evolutionary and phylogenetic studies of Strongylida species. This was the first mtDNA of any member of the genus Varestrongylus, and its comprehensive molecular characterization represents a new resource for systematic, population genetic and evolutionary biological studies of Varestrongylus lungworms in wildlife

Direct Conversion of Perovskite Thin Films into Nanowires with Kinetic Control for Flexible Optoelectronic Devices

With significant progress in the past decade, semiconductor nanowires have demonstrated unique features compared to their thin film counterparts, such as enhanced light absorption, mechanical integrity and reduced therma conductivity, etc. However, technologies of semiconductor thin film still serve as foundations of several major industries, such as electronics, displays, energy, etc. A direct path to convert thin film to nanowires can build a bridge between these two and therefore facilitate the large-scale applications of nanowires. Here, we demonstrate that methylammonium lead iodide (CH₃NH₃PbI₃) nanowires can be synthesized directly from perovskite film by a scalable conversion process. In addition, with fine kinetic control, morphologies, and diameters of these nanowires can be well-controlled. Based on these perovskite nanowires with excellent optical trapping and mechanical properties, flexible photodetectors with good sensitivity are demonstrated

Photo-oxidative degradation of methylammonium lead iodide perovskite: mechanism and protection

Although the power conversion efficiency of perovskite solar cells has exceeded 23%, the poor ambient stability of organic-inorganic halide perovskites poses a challenge for their commercialization. Comprehensive understanding of the underlying degradation mechanisms is a crucial step to seek approaches that can effectively suppress the degradation of perovskites. Herein, on the basis of extensive first-principles calculations, a three-step photo-oxidative degradation mechanism of MAPbI(3)d at the atomic level is revealed. We find that, in a dry ambient environment, the photo-generated superoxide anions (O-2(-)) first lead to fast surface oxidation. However, further oxidation of the perovskite interior is hindered by the solid oxidation product. The fresh water produced in surface oxidation leads to the hydration of the inner perovskite and eventual breakage of the MAPbI(3) lattice. We devise a practical strategy for protecting MAPbI(3) from photo-induced decomposition by anchoring hydrophobic 2-(4-fluorophenyl)propan-2-amine on the surface of MAPbI(3). The surface modification significantly retards the photo-induced decomposition