RTS hypervisor qualification for real-time systems

Virtualization is a synonym for the server and cloud computing arena. Recently, it started to be also applied to real-time embedded systems with timing constraints. However, virtualization products for data centers and desktop computing cannot be readily applied to embedded systems because of differences in requirements, use cases, and computer architecture. Bridging the gap between virtualization and real-time requirements imposes the need of real-time virtualization products. Therefore, some embedded software manufacturers have built several real-time hypervisors specialized for embedded systems. Currently, there are several commercial ones such as Greenhills INTEGRITY MultiVisor, Real-Time Systems (RTS) GmbH Hypervisor, Tenasys eVM for Windows, National Instruments Real-Time Hyper Hypervisor, and some others. This paper provides the behavior and performance results of evaluating RTS hypervisor and gives advices of its use for soft or hard real-time embedded systems

Real-time capabilities in the standard Linux Kernel: How to enable and use them?

Linux was originally designed as a general purpose operating system without consideration for real-time applications. Recently, it has become more attractive to the real-time community due to its low cost and open source approach. In order to help the real-time community, we will present in this paper the practical steps required to achieve a real-time Linux by applying the PREEMPT-RT patches which will provide Linux with these capabilities. We will also focus on some of the kernel configuration that should get attention while building the kernel in order to maintain the real-time behavior of the system during runtime. DOI: 10.17762/ijritcc2321-8169.15012

Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae

In this study, we screened adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae (E. rhusiopathiae). Inactivated cells of E. rhusiopathiae strain HG-1 were prepared as the antigen in five adjuvanted inactivated vaccines, including a mineral-oil-adjuvanted vaccine (Oli vaccine), aluminum-hydroxide-gel-adjuvanted vaccine (Alh vaccine), ISA201-biphasic-oil-emulsion-adjuvanted vaccine (ISA201 vaccine), GEL02-water-soluble-polymer-adjuvanted vaccine (GEL vaccine), and IMS1313-water-soluble-nanoparticle-adjuvanted vaccine (IMS1313 vaccine). The safety test results of subcutaneous inoculation in mice showed that Oli vaccine had the most severe side effects, with a combined score of 35, followed by the ISA201 vaccine (25 points), Alh vaccine (20 points), GEL vaccine (10 points), and IMS1313 vaccine (10 points). A dose of 1.5LD50 of strain HG-1 was used to challenge the mice intraperitoneally, 14 days after their second immunization. The protective efficacy of Oli vaccine and Alh vaccine was 100% (8/8), whereas that of the other three adjuvanted vaccines was 88% (7/8). Challenge with 2.5LD50 of strain HG-1 resulted in a 100% survival rate, demonstrating the 100% protective efficacy of the Oli vaccine, followed by the GEL vaccine (71%, 5/7), IMS1313 vaccine (57%, 4/7), ISA201 vaccine (43%, 3/7), and Alh vaccine (29%, 2/7). Challenge with 4LD50 of strain HG-1 showed 100% (7/7) protective efficacy of the Oli vaccine and 71% (5/7) protective efficacy of the GEL vaccine, whereas the protective efficacy of other three adjuvanted vaccine was 14% (1/7). The Alh and GEL vaccines were selected for comparative tests in piglets, and both caused minor side effects. A second immunization with these two adjuvanted vaccines conferred 60 and 100% protective efficacy, respectively, after the piglets were challenged via an ear vein with 8LD100 of strain HG-1. After challenge with 16LD100 of strain HG-1, the Alh and GEL vaccines showed 40% and 100% protective efficacy, respectively. Our results suggested that GEL is the optimal adjuvant for an inactivated vaccine against E. rhusiopathiae

Open-source genomic analysis of Shiga-toxin–producing E. coli O104:H4

An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic analysis of an isolate from one member of the family. This analysis involved the use of rapid, bench-top DNA sequencing technology, open-source data release, and prompt crowd-sourced analyses. In less than a week, these studies revealed that the outbreak strain belonged to an enteroaggregative E. coli lineage that had acquired genes for Shiga toxin 2 and for antibiotic resistance