Orientation to KidSkills: Teaching the Strategies and Software to Students

Further information may be found at http://kidtools.missouri.edu/AboutKTSSPrograms.phpThis handout discusses an overview of KidSkills, including aspects of program tools, age-appropriateness and strategy instruction.U.S. Department of Educatio

KidSkills: Teaching the Strategies and Software to Students

Further information may be found at http://kidtools.missouri.edu/AboutKTSSPrograms.phpThis handout discusses strategy instruction, especially as it relates to the KidSkills software.U.S. Department of Educatio

Identification of TAZ as the essential molecular switch in orchestrating SCLC phenotypic transition and metastasis

Small-cell lung cancer (SCLC) is a recalcitrant cancer characterized by high metastasis. However, the exact cell type contributing to metastasis remains elusive. Using a Rb1(L/L)/Trp53(L/L) mouse model, we identify the NCAM(hi)CD44(lo/-) subpopulation as the SCLC metastasizing cell (SMC), which is progressively transitioned from the non-metastasizing NCAM(lo)CD44(hi) cell (non-SMC). Integrative chromatin accessibility and gene expression profiling studies reveal the important role of the SWI/SNF complex, and knockout of its central component, Brg1, significantly inhibits such phenotypic transition and metastasis. Mechanistically, TAZ is silenced by the SWI/SNF complex during SCLC malignant progression, and its knockdown promotes SMC transition and metastasis. Importantly, ectopic TAZ expression reversely drives SMC-to-non-SMC transition and alleviates metastasis. Single-cell RNA-sequencing analyses identify SMC as the dominant subpopulation in human SCLC metastasis, and immunostaining data show a positive correlation between TAZ and patient prognosis. These data uncover high SCLC plasticity and identify TAZ as the key molecular switch in orchestrating SCLC phenotypic transition and metastasis