1,791 research outputs found
FDTD model performance analysis for a Cavity Slot Antenna array in a variable geometry conformal test rig
Conformal Antenna Array Modelling, FDTD Predictions and Measurements for Dual Circular Patch in Variable Geometry Conformal Antenna Array Test Rig
The moderating effect of brand orientation on inter-firm market orientation and performance
While prior research has shown that market and brand orientation
are key contributors to successful business performance, research to
date has not fully explored how inter firm collaboration for these two
key orientations can enhance business performance. The purpose of
the paper is to investigate the relationship between inter-firm market
and performance; to test for the moderating role of brand orientation
in that relationship. A total of 169 completed pairs of surveys were
collected of small and medium enterprises operating internationally
in a variety of industries in Switzerland. The results show that inter-firm
market and brand orientation are two antecedents of marketing and
financial performance. The impact of inter-firm market on marketing
and financial performance is significant when the brand orientation
is favorable. This study extends previous research by examining the
moderating role of brand orientation on inter firm market orientation,
which is important, especially for firms wanting to increase their brand
reputation by entering into partnerships with other firms. Further
research is indicated, to identify the key moderators of the driving
force of inter-firm market in relation to business performance and
the reason why maintaining a strong brand presence is important in
the international marketplace
Different Transport Pathways of Individual Precursor Proteins in Mitochondria
Transport of mitochondrial precursor proteins into mitochondria of Neurospora crassa was studied in a cellfree reconstituted system. Precursors were synthesized in a reticulocyte lysate programmed with Neurospora mRNA and transported into isolated mitochondria in the absence of protein synthesis. Uptake of the following precursors was investigated: apocytochrome c, ADP/ATP carrier and subunit 9 of the oligomycin-sensitive ATPase.
Addition of high concentrations of unlabelled chemically prepared apocytochrome c (1–10 μM) inhibited the appearance in the mitochondrial of labelled cytochrome c synthesized in vitro because the unlabelled protein dilutes the labelled one and because the translocation system has a limited capacity [apparent V is 1–3 pmol × min−1× (mg mitochondrial protein)−1]. Concentrations of added apocytochrome c exceeding the concentrations of precursor proteins synthesized in vitro by a factor of about 104 did not inhibit the transfer of ADP/ATP carrier or ATPase subunit 9 into mitochondria. Carbonylcyanide m-chlorophenylhydrazone, an uncoupler of oxidative phosphorylation, inhibited transfer in vitro of ADP/ATP carrier and of ATPase subunit 9, but not of cytochrome c.
These findings suggest that cytochrome c and the other two proteins have different import pathways into mitochondria. It can be inferred from the data presented that different 'receptors' on the mitochondrial surface mediate the specific recognition of precursor proteins by mitochondria as a first step in the transport process
Requirement of a Membrane Potential for the Posttranslational Transfer of Proteins into Mitochondsria
Posttranslational transfer of most precursor proteins into mitochondria is dependent on energization of the mitochondria. Experiments were carried out to determine whether the membrane potential or the intramitochondrial ATP is the immediate energy source. Transfer in vitro of precursors to the ADP/ATP carrier and to ATPase subunit 9 into isolated Neurospora mitochondria was investigated. Under conditions where the level of intramitochondrial ATP was high and the membrane potential was dissipated, import and processing of these precursor proteins did not take place. On the other hand, precursors were taken up and processed when the intramitochondrial ATP level was low, but the membrane potential was not dissipated. We conclude that a membrane potential is involved in the import of those mitochondrial precursor proteins which require energy for intracellular translocatio
Cell-Free Synthesis of the Mitochondrial ADP/ATP Carrier Protein of Neurospora crassa
ADP/ATP carrier protein was synthesized in heterologous cell-free systems programmed with Neurospora poly(A)-containing RNA and homologous cell-free systems from Neurospora. The apparent molecular weight of the product obtained in vitro was the same as that of the authentic mitochondrial protein. The primary translation product obtained in reticulocyte lysates starts with formylmethionine when formylated initiator methionyl-tRNA (fMet-tRNAfMet) was present. The product synthesized in vitro was released from the ribosomes into the postribosomal supernatant.
The evidence presented indicates that the ADP/ATP carrier is synthesized as a polypeptide with the same molecular weight as the mature monomeric protein and does not carry an additional sequence
Face-Sheet Quality Analysis and Thermo-Physical Property Characterization of OOA and Autoclave Panels
Increased application of polymer matrix composite (PMC) materials in large vehicle structures requires consideration of non-autoclave manufacturing technology. The NASA Composites for Exploration project, and its predecessor, Lightweight Spacecraft Structures and Materials project, were tasked with the development of materials and manufacturing processes for structures that will perform in a heavy-lift-launch vehicle environment. Both autoclave and out of autoclave processable materials were considered. Large PMC structures envisioned for such a vehicle included the payload shroud and the interstage connector. In this study, composite sandwich panels representing 1/16th segments of the barrel section of the Ares V rocket fairing were prepared as 1.8 m x 2.4 m sections of the 10 m diameter arc segment. IM7/977-3 was used as the face-sheet prepreg of the autoclave processed panels and T40-800B/5320-1 for the out of autoclave panels. The core was 49.7 kilograms per square meters (3.1 pounds per cubic feet (pcf)) aluminum honeycomb. Face-sheets were fabricated by automated tape laying 153 mm wide unidirectional tape. This work details analysis of the manufactured panels where face-sheet quality was characterized by optical microscopy, cured ply thickness measurements, acid digestion, and thermal analysis
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