Effect of Garlic Juice on Quality Changes of Oyster (Crassostrea Belcheri) Meat During Chilled Storage

Surat-thani oyster, a big and thin-shell bivalve mollusks, has been registered as Geographical Indicators, GI, as its good taste and delicacy as well as nutritious. Eaten style is raw then there is high risk to face with some disease as oyster is filter feeder. Physical, chemical, microbiological and sensory qualities after the oyster meat treated with the garlic juice at 0, 2 and 3 ml, respectively were monitored. Though initial pH of the control, untreated with garlic juice, was higher compared with the sample treated with 3 ml garlic juice, pH of it (control) was significantly lower (p5) at the end of the storag

Additional file 2: of Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis

This compressed file contains all the SILVA SeqIDs and taxonomy of the data we processed in this study. All the sequences could be accessed from SILVA with the SeqIDs. (ZIP 358 kb

Additional file 1: Table S1. of Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis

Conserved Markers used to identify sub-regions. (DOCX 12 kb

Additional file 4: Figure S2. of Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis

QIIME analysis of dataset in the manuscript. For each sub-region, we merged 89 datasets, each contained 108 sequences, into one multi-sequence fasta file. Then different barcodes were manually for different sub-regions. All the following analysis were following the standard QIIME pipeline with default parameters. The results showed no significant difference between different regions. (TIF 249 kb

Western blot analysis of tubulin (A), actin (B) and HSP-90 (C) in Ova, early larvae (EL) and old larval (OL) stages of <i>Neanthes arenaceodentata</i>.

<p>Western blot analysis of tubulin (A), actin (B) and HSP-90 (C) in Ova, early larvae (EL) and old larval (OL) stages of <i>Neanthes arenaceodentata</i>.</p

A close view of differential phosphorylation of cytoskeleton proteins (spots marked in circle) in fertilized ova and larvae of <i>Neanthes arenaceodentata.</i>

<p>A close view of differential phosphorylation of cytoskeleton proteins (spots marked in circle) in fertilized ova and larvae of <i>Neanthes arenaceodentata.</i></p

Phylogenetic tree analysis of cp20k.

<p>The full-length of the amino acid sequences were used in an alignment for phylogenetic tree calculations. The numbers indicate the occurrences of a branch point over 500 replications. The scale bar indicates the phylogenetic distances. Pf-MAPK1 was used as an out group protein.</p

Adult Bamcp20k-2 was insoluble in PBS.

<p>(A) PBS and urea soluble fractions were prepared from cyprids and adults. Cyprids or adults were extracted in PBS. After centrifugation, the supernatant was collected and the pellet was washed with the same buffer. The supernatant from each centrifugation was collected and labeled as fr1. The pellet from the final centrifugation was sequentially extracted in urea buffer. After a series of successive centrifugations mentioned above, the supernatant were collected and labeled as fr2. Fr: fraction; sup: supernatant; pelt: pellet. (B) Equal proportions from each fraction were collected for Western blot with the antibodies against Bamcp20k-1 and Bamcp20k-2. Fr: fraction.</p

Identification of abundant proteins during early development in <i>Neanthes arenaceoentata.</i>

a)<p>Accession numbers are from NCBI nr and Swiss-Prot database.</p>b)<p>significance threshold level for positive identification was p<0.05.</p>c)<p>observed MW and p<i>I</i> values were estimated from 2-DE gels.</p>d)<p>Theoretical MW and p<i>I</i> values were derived from a database search by MASCOT. PM: number of peptides matched to protein sequence; SC: sequence coverage.</p>*<p>phosphopeptides and phosphorylation sites are underlined.</p

Early developmental stages of the polychaete <i>Neanthes arenaceodentata</i>.

<p>Three developmental stages were chosen for proteomic analysis: Fertilized ova, 3–4 segmented early larva and 10–12 segmented old larva. Drawings: Three segmented larva and twelve segmented larva.</p