Determinação do sexo da Tuvira Gymnotus sp. através da análise de imagem de ultra-som

A tuvira (Gymnotus sp.) é um peixe muito conhecido no Pantanal por ser amplamente utilizado como isca viva pela pesca esportiva. Visto os problemas inerentes à sua coleta na natureza, como degradação ambiental e as condições desumanas de trabalho, faz-se necessário buscar alternativas para sua produção, como a criação em cativeiro. Entretanto, ainda não há uma técnica simples e não invasiva para a sexagem deste peixe. Deste modo, este trabalho tem por objetivo verificar a possibilidade de se utilizar a ultra-sonografia na determinação do sexo da tuvira. Foram avaliados 100 reprodutores coletados nos viveiros de reprodutores, os quais foram submetidos à análise individual de ultra-som utilizando-se um aparelho portátil (ALOKA C.O. LTD. Modelo SSD-500) com um transdutor plano de 5 MHz. Após a análise e interpretação das imagens o sexo foi determinado pelo consenso de dois observadores.bitstream/CPAP/56062/1/BP77.pdfFormato eletrônico

Integração contínua no projeto SIGI.

Integração contínua. Localização única do código fonte. Scripts de construção automatizados. Suporte a testes unitários. Uso da integração contínua no projeto SIGI. Código fonte sob controle de versão. Processo de integração contínua. Integração do SigiCliente. Integração do SigiServidor. Execução dos testes de interface Web. Avanços e melhorias.bitstream/CNPTIA/9890/1/comuntec31.pdfAcesso em: 30 maio 2008

Association of albumin or protamine to lipoplexes: enhancement of transfection and resistance to serum

The successful application of gene therapy depends on the availability of carriers to efficiently deliver genetic material into target cells. Such efficacy is strongly related to key parameters including serum resistance and protection of DNA.The complexes were tested in terms of their biological activity, in the absence or presence of serum, by following transfection activity. Interaction with plasma proteins was evaluated by immunoblotting, while cytotoxicity was assessed by the Alamar Blue assay. Extent of DNA protection was determined both by using ethidium bromide intercalation and DNase I digestion assays.Our results show that, depending on the charge ratio and on the lipid composition, albumin and protamine can be used (either individually or co-associated) to generate cationic liposome/DNA complexes fulfilling in vivo requirements, while exhibiting high levels of transfection activity. In the present work a novel cationic lipid was tested. It was demonstrated that 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (EPOPC):cholesterol (Chol) liposomes constitute a very promising carrier for gene delivery as illustrated by their enhancing effect on transfection, as compared with DOTAP-containing liposomes. Moreover, the biological activity of EPOPC-containing complexes is significantly improved upon association of albumin, even in the presence of 60% serum (namely for the 4/1 lipid/DNA charge ratio). Nevertheless, our studies also show that transfection activity mediated by DOTAP-containing complexes can be significantly enhanced upon pre-condensation of DNA with protamine.Co-association of HSA and protamine to lipoplexes ensures a high degree of DNA protection and results in high levels of transfection activity even in the presence of serum. Copyright © 2004 John Wiley & Sons, Ltd

Traduzinho XML para o modelo relacional: experiência no SIGI.

Conversão de dados de banco de dados relacional para/de o formato XML. Arquitetura do SIGI. Bancos de dados relacional e XML. A experiência no SIGI: XML e Oracle 8i. Exemplos.bitstream/CNPTIA/9194/1/COMNICADOTEC16_0.pdfAcesso em: 29 maio 2008

Involvement of MicroRNA in Microglia-Mediated Immune Response

MicroRNAs (miRNAs) are an abundant class of small noncoding RNA molecules that play an important role in the regulation of gene expression at the posttranscriptional level. Due to their ability to simultaneously modulate the fate of different genes, these molecules are particularly well suited to act as key regulators during immune cell differentiation and activation, and their dysfunction can contribute to pathological conditions associated with neuroinflammation. Recent studies have addressed the role of miRNAs in the differentiation of progenitor cells into microglia and in the activation process, aiming at clarifying the origin of adult microglia cells and the contribution of the central nervous system (CNS) environment to microglia phenotype, in health and disease. Altered expression of several miRNAs has been associated with Alzheimer’s disease, multiple sclerosis, and ischemic injury, hence strongly advocating the use of these small molecules as disease markers and new therapeutic targets. This review summarizes the recent advances in the field of miRNA-mediated regulation of microglia development and activation. We discuss the role of specific miRNAs in the maintenance and switching of microglia activation states and illustrate the potential of this class of nucleic acids both as biomarkers of inflammation and new therapeutic tools for the modulation of microglia behavior in the CNS

Cholesterol affects African swine fever virus infection

African swine fever virus (ASFV) enters cells by receptor mediated endocytosis and requires a fusion event between the viral envelope and the limiting membrane of the endosome at low pH. In order to investigate the role of cholesterol in the early stages of ASFV infection, we have studied the effect of the removal of cell and viral membrane cholesterol by cholesterol oxidase treatment of cells and virions, as well as the effect of some inhibitors of cholesterol synthesis on the infectious pathway. In addition, we have investigated viral infection in cholesterol-depleted Vero cells. Both cholesterol-depleted and cholesterol oxidase-treated Vero cells were unaltered in their ability to bind or internalize the virus, but were blocked in ASFV fusion and subsequent virus replication. Our results indicate that ASFV infection is affected by cholesterol in the target membrane.http://www.sciencedirect.com/science/article/B6T1X-3V3N06F-2/1/e52e3430df628f470dc4b0b8400b4b2

Características da inflorescência de cultivares de coqueiro nas épocas seca e chuvosa do ano.

bitstream/CPATC/19568/1/bp-21.pdfISSN 1678-1961

Produção de polpa dos frutos de cultivares de coqueiro em função da idade de colheita.

bitstream/item/35495/1/f-14.pd