Snapshots of Dengue virus (DENV) lineages spatiotemporal spread.

<p>Geographic dispersion of DENV-1 lineage I in 1999 (a1), 2001(a2), 2005 (a3), and 2008 (a4). DENV-1 lineage II: years of 1989 (b1), 1993 (b2), 1995 (b3), and 1997–2001 (b4). DENV-2 lineage I: years of 1992 (c1), 1994–1996 (c2), 1998 (c3), and 2000–2005 (c4). DENV-2 lineage II (d); DENV-2 lineage III (e); DENV-3 lineage II: years of 1998 (f1), 2000 (f2), 2002 (f3), 2004 (f4), 2006 (f5) and 2008 (f6).</p

Predictors of DENV spatial dispersal. For each potential predictor, respective Bayes factor support and conditional effect sizes (cES) are shown.

<p>Circles and bars indicate respectively the mean and 95% Bayesian credible intervals of the estimated cES, respectively. Only predictors that obtained a Bayes factor support above 3 are considered significant (highlighted in bold).</p

Most significant links of viral dispersal and mean number of jumps among regions.

<p>Bayes factor above 3 were considered significant.</p

Temporal-scaled phylogeographic DENV-1 tree.

<p>Each branch is colored according to the most probable location as inferred using a discrete phylogeographic diffusion model. Geographic locations considered are shown in the left. Phylogenetic posterior probabilities percentages are shown next to relevant nodes along with the location-state posterior support. The number of sequences falling in Brazilian monophyletic lineages (highlighted in grey) is shown in brackets. For each lineage, the mean estimated time of the most recent common ancestor (tMRCA) and respective 95% Bayesian credible intervals (BCI) are shown in a black box.</p

Temporal-scaled phylogeographic DENV-3 tree.

<p>Each branch is colored according to the most probable location as inferred using a discrete phylogeographic diffusion model. Geographic locations considered are shown in the left. Phylogenetic posterior probabilities percentages are shown next to relevant nodes along with the location-state posterior support. The number of sequences falling in Brazilian monophyletic lineages (highlighted in grey) is shown in brackets. For each lineage, the mean estimated time of the most recent common ancestor (tMRCA) and respective 95% Bayesian credible intervals (BCI) are shown in a black box.</p

Temporal-scaled phylogeographic DENV-2 tree.

<p>Each branch is colored according to the most probable location as inferred using a discrete phylogeographic diffusion model. Geographic locations considered are shown in the left. Phylogenetic posterior probabilities percentages are shown next to relevant nodes along with the location-state posterior support. The number of sequences falling in Brazilian monophyletic lineages (highlighted in grey) is shown in brackets. For each lineage, the mean estimated time of the most recent common ancestor (tMRCA) and respective 95% Bayesian credible intervals (BCI) are shown in a black box.</p

DENV strains used for complete genome sequencing, phylogeographic and spatial-temporal analyses according to its serotype, strain, source of isolation and geographic location (Federal states or cities) in Brazil.

<p>*City of Belém, capital of Pará State, Northern Brazil;</p><p>?: year of isolation not provided.</p

Mean ages of the MRCA of Brazilian circulating DENV lineages, most probable origins and substitution rates.

<p>Notes: BCI: Bayesian credible interval; PP: Posterior Probability; s/s/y: substitution per site per year. Note that numbering of lineages is convenient and has been ordered according to the estimated time of arrival to Brazil. Dispersal rates estimated according to the best-fit continuous diffusion model (DENV-1: RRW gamma, DENV-2: RRW Cauchy, DENV-3: RRW: Gamma).</p

Dispersal rates of DENV-1, DENV-2 and DENV-3 Brazilian lineages.

<p>Dispersal rates in units of km per day (km/d) were estimated according to the best-fitting continuous diffusion model (Supplementary <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002769#pntd.0002769.s005" target="_blank">Table S4</a>).</p

Epidemiological Data: Numbers of suspected ZIKV cases and suspected microcephaly cases per state and per epidemiological week.

Contains 1) CSV file with number suspected ZIKV cases from January 2015 to the end of December 2015; 2) CSV file with number of suspected microcephaly cases from January 2015 to the first week of January 2016. Numbers correspond to suspected microcephaly cases at week 20 of pregnancy; 3) CSV file with codes of state of residence and municipality of residence in Brazil; and 4) R scripts for correlation analysis described in SI Section 1.5