Dectin-1-Mediated Production of Pro-Inflammatory Cytokines Induced by Yeast β-Glucans in Bovine Monocytes

Yeast-derived products containing β-glucans have long been used as feed supplements in domesticated animals in an attempt to increase immunity. β-glucans are mainly recognized by the cell surface receptor CLEC7A, also designated Dectin-1. Although the immune mechanisms elicited through Dectin-1 activation have been studied in detail in mice and humans, they are poorly understood in other species. Here, we evaluated the response of bovine monocytes to soluble and particulate purified β-glucans, and also to Zymosan. Our results show that particulate, but not soluble β-glucans, can upregulate the surface expression of costimulatory molecules CD80 and CD86 on bovine monocytes. In addition, stimulated cells increased production of IL-8 and of TNF, IL1B, and IL6 mRNA expression, in a dose-dependent manner, which correlated positively with CLEC7A gene expression. Production of IL-8 and TNF expression decreased significantly after CLEC7A knockdown using two different pairs of siRNAs. Overall, we demonstrated here that bovine monocytes respond to particulate β-glucans, through Dectin-1, by increasing the expression of pro-inflammatory cytokines. Our data support further studies in cattle on the induction of trained immunity using dietary β-glucans.This work received financial support from PT national funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through the project UIDB/info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB/50006/2020/PT. AP was supported by FCT phD grant PD/BDE/135540/2018. AC was supported by FCT Individual CEEC 2017 Assistant Researcher Grant CEECIND/01514/2017. MM was supported by FCT through program DL 57/2016 – Norma transitória (SFRH/BPD/70716/2010)

Associação entre comportamentos de risco à saúde de pais e adolescentes em escolares de zona rural de um município do Sul do Brasil Association between health risk behaviors in parents and adolescents in a rural area in southern Brazil

Objetivou-se avaliar a associação entre comportamentos de risco à saúde dos pais e adolescentes escolares de zona rural. Participaram 377 escolares entre 10 e 18 anos e 338 pais. Foram analisados: nível de atividade física no lazer e deslocamento, hábitos de alimentação inadequados, excesso de peso, uso de cigarros e consumo de bebidas alcoólicas. A prevalência de relato de uso de cigarro e álcool nos últimos 30 dias entre os jovens foi de 6,7% e 27%, respectivamente. Quarenta e cinco por cento dos adolescentes não atingiram o escore de atividade física. A adesão aos passos para uma alimentação saudável foi baixa. A atividade física dos adolescentes esteve relacionada de forma positiva com a dos pais, e o consumo de álcool da mãe se associou de forma positiva com o consumo do adolescente. Para os outros comportamentos, não houve associação significativa. Na análise estratificada por sexo, o nível de atividade física do pai se associou ao nível de atividade física das meninas. O comportamento de risco à saúde dos pais associa-se ao mesmo comportamento dos adolescentes de zona rural.<br>This article aimed to assess the association between health risk behaviors in parents and adolescents in a rural area. The sample included 377 schoolchildren 10 to 18 years of age and 338 parents. The following variables were analyzed: level of leisure-time and commuting physical activity, inadequate eating habits, overweight/obesity, smoking, and alcohol consumption. Prevalence rates for smoking and alcohol consumption by adolescents in the previous 30 days were 6.7% and 27%, respectively. Forty-five percent of adolescents failed to reach the target physical activity score. Adherence to steps to healthy eating was low. Physical activity in adolescents was positively associated with that of parents, and adolescent drinking was associated with maternal alcohol consumption. There was no significant association for other behaviors. In the analysis stratified by gender, level of physical exercise in female adolescents was associated with that of fathers. Parental health risk behaviors were associated with the behaviors in the adolescent children in this rural area