Pathogenesis of feline enteric coronavirus infection.

Fifty-one specific pathogen-free (SPF) cats 10 weeks to 13 years of age were infected with a cat-to-cat fecal-oral passed strain of feline enteric coronavirus (FECV). Clinical signs ranged from unapparent to a mild and self-limiting diarrhea. Twenty-nine of these cats were FECV naïve before infection and followed sequentially for fecal virus shedding and antibody responses over a period of 8-48 months. Fecal shedding, as determined by real-time polymerase chain reaction (RT-PCR) from rectal swabs, appeared within a week and was significantly higher in kittens than older cats. FECV shedding remained at high levels for 2-10 months before eventually evolving into one of three excretion patterns. Eleven cats shed the virus persistently at varying levels over an observation period of 9-24 months. Eleven cats appeared to have periods of virus shedding interlaced with periods of non-shedding (intermittent or recurrent shedders), and seven cats ceased shedding after 5-19 months (average 12 months). There was no change in the patterns of virus shedding among cats that were excreting FECV at the time of a secondary challenge exposure. Four cats, which had ceased shedding, re-manifested a primary type infection when secondarily infected. Cats with higher feline coronavirus (FCoV) antibody titers were significantly more likely to shed virus, while cats with lower titers were significantly less likely to be shedding. Twenty-two kittens born to experimentally infected project queens began shedding virus spontaneously, but never before 9-10 weeks of age. Natural kittenhood infections appeared to be low grade and abortive. However, a characteristic primary type infection occurred following experimental infection with FECV at 12-15 weeks of age. Pregnancy, parturition and lactation had no influence on fecal shedding by queens. Methylprednisolone acetate treatment did not induce non-shedders to shed and shedders to increase shedding

Adiabatic Elimination in a Lambda System

This paper deals with different ways to extract the effective two-dimensional lower level dynamics of a lambda system excited by off-resonant laser beams. We present a commonly used procedure for elimination of the upper level, and we show that it may lead to ambiguous results. To overcome this problem and better understand the applicability conditions of this scheme, we review two rigorous methods which allow us both to derive an unambiguous effective two-level Hamiltonian of the system and to quantify the accuracy of the approximation achieved: the first one relies on the exact solution of the Schrodinger equation, while the second one resorts to the Green's function formalism and the Feshbach projection operator technique.Comment: 14 pages, 3 figure

Finite element analysis of stress distribution and the effects of geometry in a laser-generated single-stage ceramic tile grout seal using ANSYS

Optimisation of the geometry (curvature of the vitrified enamel layer) of a laser-generated single-stage ceramic tile grout seal has carried out with a finite element (FE) model. The overall load bearing capacities and load-displacement plots of three selected geometries were determined experimentally by the indentation technique. Simultaneously, a FE model was developed utilising the commercial ANSYS package to simulate the indentation. Although the load-displacement plots generated by the FE model consistently displayed stiffer identities than the experimentally obtained results, there was reasonably close agreement between the two sets of results. Stress distribution profiles of the three FE models at failure loads were analysed and correlated so as to draw an implication on the prediction of a catastrophic failure through an analysis of FE-generated stress distribution profiles. It was observed that although increased curvatures of the vitrified enamel layer do enhance the overall load-bearing capacity of the single-stage ceramic tile grout seal and bring about a lower nominal stress, there is a higher build up in stress concentration at the apex that would inevitably reduce the load-bearing capacity of the enamel glaze. Consequently, the optimum geometry of the vitrified enamel layer was determined to be flat

Bovine Respiratory Syncytial Virus (BRSV) Pneumonia in Beef Calf Herds Despite Vaccination

The present report describes the clinical, pathological, serological and virological findings in calves from 2 larger Danish beef herds experiencing outbreaks of pneumonia. The calves had been vaccinated with an inactivated bovine respiratory syncytial virus (BRSV) vaccine 2 months prior to the outbreak. The clinical signs comprised nasal discharge, pyrexia, cough and increased respiratory rates. A total of 28 calves died in the 2 herds. The laboratory investigations revealed that BRSV was involved and probably initiated both outbreaks. Furthermore, the serological results suggested that the vaccine induced only sparse levels of antibodies probably due to the presence of maternally derived antibodies at the time of vaccination. Necropsy findings in 5 calves revealed changes typical for infectious pneumonia with involvment of BRSV. In conclusion, vaccination of calves against BRSV in 2 Danish beef herds failed to protect the calves against severe or even fatal BRSV mediated respiratory disease 2 months later

The nucleoside analog GS-441524 strongly inhibits feline infectious peritonitis (FIP) virus in tissue culture and experimental cat infection studies.

Feline infectious peritonitis (FIP) is a common and highly lethal coronavirus disease of domestic cats. Recent studies of diseases caused by several RNA viruses in people and other species indicate that antiviral therapy may be effective against FIP in cats. The small molecule nucleoside analog GS-441524 is a molecular precursor to a pharmacologically active nucleoside triphosphate molecule. These analogs act as an alternative substrate and RNA-chain terminator of viral RNA dependent RNA polymerase. We determined that GS-441524 was non-toxic in feline cells at concentrations as high as 100 uM and effectively inhibited FIPV replication in cultured CRFK cells and in naturally infected feline peritoneal macrophages at concentrations as low as 1 uM. We determined the pharmacokinetics of GS-441524 in cats in vivo and established a dosage that would sustain effective blood levels for 24 h. In an experimental FIPV infection of cats, GS-441524 treatment caused a rapid reversal of disease signs and return to normality with as little as two weeks of treatment in 10/10 cats and with no apparent toxicity

Commuting self-adjoint extensions of symmetric operators defined from the partial derivatives

We consider the problem of finding commuting self-adjoint extensions of the partial derivatives {(1/i)(\partial/\partial x_j):j=1,...,d} with domain C_c^\infty(\Omega) where the self-adjointness is defined relative to L^2(\Omega), and \Omega is a given open subset of R^d. The measure on \Omega is Lebesgue measure on R^d restricted to \Omega. The problem originates with I.E. Segal and B. Fuglede, and is difficult in general. In this paper, we provide a representation-theoretic answer in the special case when \Omega=I\times\Omega_2 and I is an open interval. We then apply the results to the case when \Omega is a d-cube, I^d, and we describe possible subsets \Lambda of R^d such that {e^(i2\pi\lambda \dot x) restricted to I^d:\lambda\in\Lambda} is an orthonormal basis in L^2(I^d).Comment: LaTeX2e amsart class, 18 pages, 2 figures; PACS numbers 02.20.Km, 02.30.Nw, 02.30.Tb, 02.60.-x, 03.65.-w, 03.65.Bz, 03.65.Db, 61.12.Bt, 61.44.B

A new sea ice albedo scheme including melt ponds for ECHAM5 general circulation model

Today we experience an accelerated melting of sea ice in the Arctic which global circulation models are inadequate to predict. We believe one of the reasons is the shortcomings in the sea ice albedo schemes for these models. This paper investigates a physically based sea ice albedo scheme for ECHAM5 GCM, which separates between snow-covered sea ice, bare sea ice, melt ponds, and open water (separately for the albedos and albedo fractions). The new albedo scheme includes important components such as albedo decay due to snow aging, bare sea ice albedo dependent on the ice thickness, and a melt pond albedo dependent on the melt pond depth. The explicit treatment of melt pond albedos has, to our knowledge, not been included in general circulation models before and represents a substantial improvement when simulating the annual cycle of sea ice albedo. The new albedo scheme overall reduces the sea ice albedo both in winter, because of snow aging, and in summer, because of melt ponds. The reduced sea ice albedo leads to overall reduced sea ice thickness, concentration, and volume, with large temporal and spatial variations. In the Northern Hemisphere in March, some areas experience increased albedo, resulting in thicker sea ice and higher ice concentration, but in August the pattern is spatially homogeneous, with reduced albedo, thickness, and concentrations for all areas where the new scheme has a significant effect. [References: 57