The role of microRNAs in thyroid carcinomas

Thyroid cancers (TCs) are the most common malignancies of endocrine organs. They originate from cells of different origin within the thyroid gland, which is located at the base of the neck. Several forms of TCs have been classified and great variability is observed in molecular, cellular and clinical features. The most common forms have favorable prognosis but a number of very aggressive TCs, which are characterized by a less differentiated cellular phenotype, have no effective treatment at the moment. While TC causes are not completely understood, many genetic factors involved in their onset have been discovered. In particular, activating mutations of BRAF, RET or RAS genes are known to be specifically associated with TC initiation, progression and outcome. The involvement of microRNAs in thyroid neoplasms has recently changed the paradigm for biomarker discovery in TC, suggesting that these small non-coding RNAs could be used to develop, refine or strengthen strategies for diagnosis and management of TCs. In this review, the importance of microRNA profiling in TC is explored suggesting that these molecules can be included in procedures that can perform better than any known clinical index in the identification of adverse outcomes

Experimental characterization of tensile strength of steel and fibre rovings also under environmental conditioning

Abstract The efficiency of the strengthening techniques by externally applied materials can be improved enhancing the debonding strength of the reinforcement from the support by the use of connectors (anchor spikes) consisting of unidirectional bundles of fibres embedded in concrete or masonry by means of organic or inorganic matrices. The use of connectors is suggested in various codes and guidelines of strengthening techniques by composite materials and provisions for their application are given, but currently there are no details for the qualification of the material. In order to investigate anchor spikes made of glass, basalt, aramid, carbon, PBO and steel, a large experimental campaign was carried out at the Materials and Structures Laboratory of the University of Sannio. The tests allowed to evaluate the mechanical characteristics (tensile strength, modulus of elasticity, deformation at the maximum load) of the anchor spikes constituted by only dry fibres, not impregnated, also as a result of environmental conditioning such as freezing and thawing, controlled humidity, alkaline and saline environment

Finite Element Modeling of Bond Behavior of FRP and Steel Plates

Strengthening systems for existing reinforced concrete (RC) structures are increasingly needed due to several problems such as degradation of materials over the time, underdesign, serviceability or seismic upgrading, or new code requirements. In the last decades, strengthening by fibers composite materials applied with various techniques (FRP, FRCM, NSM) were largely investigated and theoretical formulations have been introduced in national and international design guidelines. Although they are an excellent strengthening solution, steel plates may represent still a valid traditional alternative, due to low costs, ductile stress-strain behavior, simple and fast mounting with possibility of reusing the material. Guidelines for a correct design are still lack and, therefore, detailed models and design formulas are needed. In this paper, the bond behavior at the plate-concrete interface, which plays a key role for the effectiveness of the strengthening system, is analyzed by means of 3D finite element models calibrated on experimental results available in literature. Parametric analyses were carried out by changing some meaningful parameters

Assessment of non linear bond laws for NSM systems in concrete elements

In this paper the numerical FEM model developed to simulate the behaviour of the NSM strengthening system for concrete elements is presented. The plane model introduces the non linear bond law of the system by an interface element between the composite reinforcement and the concrete considered linear. The results of two experimental programs are analysed and used to calibrate the parameters of the bond shear-slip relationship by means the numerical model. The procedure is based on the inverse analysis of the experimental data in order to have the better fitting of the global pull-out load-displacement curves.(undefined

Efficiency of CFRP NSM strips and EBR laminates for flexural strengthening of RC beams

The efficiency of Fiber Reinforced Polymer (FRP) materials for strengthening existing Reinforced Concrete (RC) structures according to the Near Surface Mounted (NSM) technique can be greater than the External Bonded Reinforcement (EBR) technique since the tensile strength of the FRP materials is in general better exploited. Firstly, this paper deals with analyzing the effect of the loading pattern on RC beams strengthened with both types of strengthening technique; in particular, two loading patterns have been used for the experimental tests on simple supported beams: 1) a four points bending scheme, and 2) a scheme with distributed loads, in order to check the sensitivity of failure modes and ultimate loads to different distributions of bending moment and shear along the beam. Then, a comparison between the results of flexural tests on RC beams strengthened with both NSM and EBR techniques is dealt with

Engineering properties of geopolymer concrete: a review

Geopolymer concrete (GPC) could be a solution that uses a cementless binder and recycled materials for producing concrete, while reducing the carbon dioxide emission and the demand for raw materials. In addition to the environmental aspect, previous studies on GPC showed that it can achieve mechanical characteristics higher than those of ordinary Portland concrete (OPC) such as greater strength a few days after casting, and it can be suitable for structural applications. In this paper, the state-of-the-art review of GPC is presented through an extensive literature analysis to determine the most recent information regarding the engineering properties of geopolymer concrete and the critical issues that prevent its widespread use and to put forward suggestions for future research. In particular, the physical properties in both fresh and hardened states and the mechanical characteristics are investigated; the structural performance of geopolymer concrete elements is also outlined

Assessment of nonlinear bond laws for near-surface-mounted systems in concrete elements

This paper presents a numerical plane Finite Element (FE) Model for use in simulating the behaviour of different types of Near Surface Mounted (NSM) Fibre Reinforced Plastic (FRP) strengthening systems for concrete elements. Based on a nonlinear bond law for simulating the behaviour of the FRP reinforcement-adhesive-concrete interface, the model employs an interface element between the NSM FRP reinforcement and the concrete. The results of two different experimental programs, both dealing with 'bond tests' but with distinct set-ups, are briefly summarised and analysed. The main objective of this research is to assess the values of the parameters that define the nonlinear bond laws for each type of FRP reinforcement tested. This assessment was accomplished by inverse analysis, fitting numerically the pullout load–displacement curves that were experimentally recorded. The effect of bond length on different types of NSM FRP reinforcement is assessed. Finally, the bond behaviour in the transverse plane is examined too.Fundação para a Ciência e a Tecnologia (FCT

Reduced expression of THRβ in papillary thyroid carcinomas: relationship with BRAF mutation, aggressiveness and miR expression

Purpose Down-regulation of thyroid hormone receptor beta (THRβ) gene has been described in several human malignancies, including thyroid cancer. In this study, we analyzed THRβ mRNA expression in surgical specimens from a series of human papillary thyroid carcinomas (PTCs), characterized by their genotypic and clinical–biological features. Methods Thirty-six PTCs were divided into two groups according to the 2009 American Thyroid Association risk classification (17 low, 19 intermediate), and each group was divided into subgroups based on the presence or absence of the BRAFV600E mutation (21 BRAF mutated, 15 BRAF wild type). Gene expression was analyzed using fluidic cards containing probes and primers specific for the THRβ gene, as well as for genes of thyroperoxidase (TPO), sodium/iodide symporter (NIS), thyroglobulin (Tg) and thyroid stimulating hormone receptor (TSH-R) and for some miRNAs involved in thyroid neoplasia and targeting THRβ. The mRNA levels of each tumor tissue were compared with their correspondent normal counterpart. Results THRβ transcript was down-regulated in all PTCs examined. No significant differences were found between intermediate- vs low-risk PTCs patients, and BRAF-mutated vs BRAF wild-type groups. THRβ expression was directly correlated with NIS, TPO, Tg and TSH-R, and inversely correlated to miR-21, -146a, -181a and -221 expression. Conclusions Our results demonstrate that down-regulation of THRβ is a common feature of PTCs. While it is not associated with a more aggressive phenotype of PTC, it correlates with the reduction of all the markers of differentiation and is associated with overexpression of some miRNAs supposed to play a role in thyroid tumorigenesis