Ecological Indicator Development, Integration and Knowledge Mapping

The overall goals of this project were: (1) to develop a microbiological ecological indicator that would describe military land disturbance, (2) integrate previously collected ecological indicator data from five separate research teams, and (3) produce knowledge maps with the resulting information that illustrates how the selected indicators are involved in ecosystem processes. To address goal one, soil samples were obtained from four levels of military traffic (reference, light, moderate, and heavy) with an additional set of samples taken from previously damaged areas. Using the soil microbial biomass and community composition as ecological indicators, reproducible changes showed increasing traffic disturbance decreases soil viable biomass, biomarkers for microeukaryotes and Gram-negative bacteria, while increasing the proportions of aerobic Gram-positive bacterial and actinomycete biomarkers. To address the second goal, ecological indicator data was collected by five separate research teams. Landmanagement categories (LMCs) were developed that described the uses or causes of the ecological effect from military use(s) of land. A mechanism of multiple solutions was developed that combined the results and tested the efficacy of the proposed indicators. Results from the integration effort showed that Soil A horizon depth and soil compaction were important soil physical indicators of military land disturbance. Soil Nitrogen and Carbon content were important soil chemical indicators of land use. Soil mineralization rate, soil respiration, microbial composition and Beta Glucosidase activity were important microbiological indicators. Important plant indicators included tree stand age, canopy iv cover, understory cover, plant life form and legumes. To address the third goal several knowledge maps were developed, and the results from the integration of indicator data were pooled and studied for the relationships between them. By displaying the indicators in this fashion, it was hoped that the knowledge of what the indicators represent to the functioning of the ecological system could be understood. For the practitioner, this knowledge should lead to actionable products or at least a better understanding of what is being measured and how it relates to broader ecosystem dynamics

Evaluation of a soil-based pesticide wastewater disposal system using a combination of chemical parameters and microbial biomarker techniques

Non-point source pollution from agricultural pesticides is a growing problem in surface and groundwater contamination. Even though an operator may follow best management practices during application, there remains the residual pesticide contaminated wastewater (tank rinsates). Mixed waste streams of commonly used East Tennessee herbiddes at various concentrations were tested in soil columns which simulated a Soil Bed Bioreactor, and then repeated with the addition two other insecticides. Pesticide dissipation, soil chemistry, and microbial community response were of prime interest. Results indicate that pesticide dissipation behavior in the bioreactor is similar to that in the field, and that of the seven pesticides analyzed in this experiment, six significantly dissipated at low and moderate concentrations within 30 days. As pesticide concentration in the bioreactor increased the microbial community structure shifted to one that was resistant, a shift that may ultimately be involved in detoxification. These data demonstrate and validate the utility of this technology to concentrate and dissipate pesticide rinsates, and show that these methods could become an important tool for farmers and custom applicators

Plant Diversity, Soil Microbial Communities, And Ecosystem Function: Are There Any Links?

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/117152/1/ecy20038482042.pd

Modulation of anabolic and catabolic responses via a porous polymer scaffold manufactured using thermally induced phase separation

We describe two studies encompassing the iterative refinement of a polymer-based rhBMP-2 delivery system for bone tissue engineering. Firstly, we compared the boneforming capacity of porous poly(D,L-lactic-co-glycolic acid) (PLGA) scaffolds produced by thermally induced phase separation (TIPS) with non-porous solvent cast poly(D,L-lactic acid) (PDLLA) used previously. Secondly, we examined the potential synergy between rhBMP-2 and local bisphosphonate in the PLGA scaffold system. In vivo ectopic bone formation studies were performed in C57BL6/J mice. Polymer scaffolds containing 0, 5, 10 or 20 μg rhBMP-2 were inserted into the dorsal musculature. At all rhBMP-2 doses, porous PLGA produced significantly higher bone volume (BV, mm) than the solid PDLLA scaffolds. Next, porous PLGA scaffolds containing 10μg rhBMP-2 ±0.2, or 2μg zoledronic acid (ZA) were inserted into the hind-limb musculature. Co-delivery of local 10μg rhBMP-2/2μg ZA significantly augmented bone formation compared with rhBMP-2 alone (400 % BV increase, p < 0.01). Hydroxyapatite microparticle (HAp) addition (2% w/w) to the 10μg rhBMP-2/0.2μg ZA group increased BV (200 %, p < 0.01). We propose that this was due to controlled ZA release of HAp-bound ZA. Consistent with this, elution analyses showed that HAp addition did not alter the rhBMP-2 elution, but delayed ZA release. Moreover, 2 % w/w HAp addition reduced the scaffold's compressive properties, but did not alter ease of surgical handling. In summary, our data show that refinement of the polymer selection and scaffold fabrication can enhance rhBMP-2 induced bone formation in our bone tissue engineering implant, and this can be further optimised by the local co-delivery of ZA/HAp

A collagen-hydroxyapatite scaffold allows for binding and co-delivery of recombinant bone morphogenetic proteins and bisphosphonates.

An emerging paradigm in orthopedics is that a bone-healing outcome is the product of the anabolic (bone-forming) and catabolic (bone-resorbing) outcomes. Recently, surgical and tissue engineering strategies have emerged that combine recombinant human bone morphogenetic proteins (rhBMPs) and bisphosphonates (BPs) in order to maximize anabolism and minimize catabolism. Collagen-based scaffolds that are the current surgical standard can bind rhBMPs, but not BPs. We hypothesized that a biomimetic collagen-hydroxyapatite (CHA) scaffold would bind both agents and produce superior in vivo outcomes. Consistent with this concept, in vitro elution studies utilizing rhBMP-2 ELISA assays and scintillation counting of (14)C-radiolabeled zoledronic acid (ZA) confirmed delayed release of both agents from the CHA scaffold. Next, scaffolds were tested for their capacity to form ectopic bone after surgical implantation into the rat hind limb. Using CHA, a significant 6-fold increase in bone volume was seen in rhBMP-2/ZA groups compared to rhBMP-2 alone, confirming the ability of ZA to enhance rhBMP-2 bone formation. CHA scaffolds were found to be capable of generating mineralized tissue in the absence of rhBMP-2. This study has implications for future clinical treatments of critical bone defects. It demonstrates the relative advantages of co-delivering anabolic and anti-catabolic agents using a multicomponent scaffold system

The Peaks Formalism and the Formation of Cold Dark Matter Haloes

We use two cosmological simulations of structure formation to study the conditions under which dark matter haloes emerge from the linear density field. Our analysis focuses on matching sites of halo collapse to local density maxima, or "peaks", in the initial conditions of the simulations and provides a crucial test of the central ansatz of the peaks formalism. By identifying peaks on a variety of smoothed, linearly extrapolated density fields we demonstrate that as many as ~70% of well-resolved dark matter haloes form preferentially near peaks whose characteristic masses are similar to that of the halo, with more massive haloes showing a stronger tendency to reside near peaks initially. We identify a small but significant fraction of haloes that appear to evolve from peaks of substantially lower mass than that of the halo itself. We refer to these as "peakless haloes" for convenience. By contrasting directly the properties of these objects with the bulk of the proto-halo population we find two clear differences: 1) their initial shapes are significantly flatter and more elongated than the predominantly triaxial majority, and 2) they are, on average, more strongly compressed by tidal forces associated with their surrounding large scale structure. Using the two-point correlation function we show that peakless haloes tend to emerge from highly clustered regions of the initial density field implying that, at fixed mass, the accretion geometry and mass accretion histories of haloes in highly clustered environments differ significantly from those in the field. This may have important implications for understanding the origin of the halo assembly bias, of galaxy properties in dense environments and how environment affects the morphological transformation of galaxies near groups and rich galaxy clusters.Comment: 13 pages, 11 figures, published in MNRA

The spatial and velocity bias of linear density peaks and proto-haloes in the Lambda cold dark matter cosmology

We use high resolution N-body simulations to investigate the Lagrangian bias of cold dark matter haloes within the LCDM cosmology. Our analysis focuses on "proto-haloes", which we identify in the simulation initial conditions with the subsets of particles belonging to individual redshift-zero haloes. We then calculate the number-density and velocity-divergence fields of proto-haloes and estimate their auto spectral densities. We also measure the corresponding cross spectral densities with the linear matter distribution. We use our results to test a Lagrangian-bias model presented by Desjacques and Sheth which is based on the assumption that haloes form out of local density maxima of a specific height. Our comparison validates the predicted functional form for the scale-dependence of the bias for both the density and velocity fields. We also show that the bias coefficients are accurately predicted for the velocity divergence. On the contrary, the theoretical values for the density bias parameters do not accurately match the numerical results as a function of halo mass. This is likely due to the simplistic assumptions that relate virialized haloes to density peaks of a given height in the model. We also detect appreciable stochasticity for the Lagrangian density bias, even on very large scales. These are not included in the model at leading order but correspond to higher order corrections.Comment: 10 pages, 4 figures. Matches version accepted for publication in MNRA

Serum magnesium and risk of sudden cardiac death in the Atherosclerosis Risk in Communities (ARIC) Study

We hypothesized that serum magnesium (Mg) is associated with increased risk of sudden cardiac death (SCD)

The influence of vernalization and daylength on expression of flowering-time genes in the shoot apex and leaves of barley (Hordeum vulgare).

Responses to prolonged low-temperature treatment of imbibed seeds (vernalization) were examined in barley (Hordeum vulgare). These occurred in two phases: the perception of prolonged cold, which occurred gradually at low temperatures, and the acceleration of reproductive development, which occurred after vernalization. Expression of the VERNALIZATION1 gene (HvVRN1) increased gradually in germinating seedlings during vernalization, both at the shoot apex and in the developing leaves. This occurred in darkness, independently of VERNALIZATION2 (HvVRN2), consistent with the hypothesis that expression of HvVRN1 is induced by prolonged cold independently of daylength flowering-response pathways. After vernalization, expression of HvVRN1 was maintained in the shoot apex and leaves. This was associated with accelerated inflorescence initiation and with down-regulation of HvVRN2 in the leaves. The largest determinant of HvVRN1 expression levels in vernalized plants was the length of seed vernalization treatment. Daylength did not influence HvVRN1 expression levels in shoot apices and typically did not affect expression in leaves. In the leaves of plants that had experienced a saturating seed vernalization treatment, expression of HvVRN1 was higher in long days, however. HvFT1 was expressed in the leaves of these plants in long days, which might account for the elevated HvVRN1 expression. Long-day up-regulation of HvVRN1 was not required for inflorescence initiation, but might accelerate subsequent stages of inflorescence development. Similar responses to seed vernalization were also observed in wheat (Triticum aestivum). These data support the hypothesis that VRN1 is induced by cold during winter to promote spring flowering in vernalization-responsive cereals