Retail Trade on the U.S.-Mexico Border During the NAFTA Implementation Era

When NAFTA was implemented in 1994, there was a general expectation that it would hurt U.S. retailers along the U.S.-Mexico border. This paper asks whether there was a significant change in the pattern of retail trade in border MSAs in the years surrounding NAFTA's implementation. Data from MSAs in the four border states are analyzed. After controlling for other potential influences on retail trade, there remained a statistically significant change in the pattern of retail trade between 1992 and 1997. The changes cannot be unquestionably attributed to NAFTA but do suggest that NAFTA had a negative influence on retail sales on the U.S. side of the border. Copyright 2004 Gatton College of Business and Economics, University of Kentucky..

Enabling technologies for manipulating multiple genes on complex pathways

Many complex biochemical pathways in plants have now been manipulated genetically, usually by suppression or over-expression of single genes. Further exploitation of the potential for plant genetic manipulation, both as a research tool and as a vehicle for plant biotechnology, will require the co-ordinate manipulation of multiple genes on a pathway. This goal is currently very difficult to achieve. A number of approaches have been taken to combine or 'pyramid' transgenes in one plant and have met with varying degrees of success. These approaches include sexual crossing, re-transformation, co-transformation and the use of linked transgenes. Novel, alternative 'enabling' technologies are also being developed that aim to use single transgenes to manipulate the expression of multiple genes. A chimeric transgene with linked partial gene sequences placed under the control of a single promoter can be used to co-ordinately suppress numerous plant endogenous genes. Constructs modelled on viral polyproteins can be used to simultaneously introduce multiple protein-coding genes into plant cells. In the course of our work on the lignin biosynthetic pathway, we have tested both conventional and novel methods for achieving co-ordinate suppression or over-expression of up to three plant lignin genes. In this article we review the literature concerning the manipulation of multiple genes in plants. We also report on our own experiences and results using different methods to perform directed manipulation of lignin biosynthesis in tobacco.</p