Identification of “Kratom” (Mitragyna speciosa) Alkaloids in Commercially Available Products

“Kratom” is the common name for the botanical mitragyna speciosa. It is a tree native to Southeast Asia in which leaves contain the psychoactive alkaloids mitragynine and 7-hydroxymitragynine. Kratom is often ingested as teas, chewed, or smoked. It acts as a stimulant in small doses and as an opioid in large doses. Overdoses can result in vomiting, seizures, and death. Recently the Drug Enforcement Agency (DEA) placed Kratom on Schedule 1, but, due to public outcry, it was almost immediately removed. Eleven kratom based products were obtained from various tobacco shops, “headshops” and via the internet including: Choice brand capsule and powder, Krave brand capsule, Lucky brand powder, King Kratom and Purple Haze e-liquids with 0 mg nicotine and 12 mg nicotine, Mojo brand capsule, O.P.M.S Liquid Kratom concentrate, and a K. Kratom chocolate bar. These products and methanol extracted samples were analyzed for psychoactive alkaloids and other components using an AccuTOF Direct Analysis in Real Time Mass Spectrometry (DART-MS) and Gas Chromatography-Mass Spectrometry (GC-MS). The psychoactive alkaloids mitragynine and 7-hydroxymitragynine along with four other alkaloids, corynantheidine, corynoxine, paynantheine, and speciofoline, were identified in all 11 products. Unregulated commercial products made from kratom contain psychoactive alkaloids such as mitragynine and 7-hydroxymitragynine. They are readily available in a variety of forms and may be prone to abuse.https://scholarscompass.vcu.edu/uresposters/1246/thumbnail.jp

Expressed emotion and adjustment in families with children with autism spectrum conditions

Children with Autism Spectrum Conditions (ASC) present with social and communication deficits, and patterns of restricted and repetitive behaviours (APA, 2000). These difficulties have significant impacts for families, including increased levels of stress and mental health problems when compared to parents raising children with other developmental or intellectual conditions (e.g. Singer, 2006). Research has sought to understand this impact and to identify the factors that place parents at risk for poor adjustment outcomes so that interventions can be effectively tailored to facilitate improved outcomes for families. This thesis reviews the adjustment literature and considers how it fits within a widely used model of adjustment, the Double ABCX model (McCubbin & Patterson, 1983). It then goes on to consider the importance of the parent-child relationship for adjustment via the construct of Expressed Emotion (EE), and explores how this literature adds to our understanding of the adjustment process in families of children with ASC.Next, the role of EE in the adjustment process of families of children with ASC is investigated. A mediation model is proposed that incorporates a measure of EE within the Double ABCX model. The validity of this model is tested in a sample of primary caregivers who have children with ASC. Whilst the results of the study indicate that EE does not have a mediating role in the adjustment of these families, the study has a number of limitations and suggestions for future research that are discussed in detail

Forced Confessions of a Lover

Title of Document: FORCED CONFESSION OF A LOVER Alexander R Peace, MFA, 2012 Directed By: Professor WC Richardson, Department of Art I believe in Painting, the moment the historic and the autobiographic collapse within a single frame

The Forensic Characterization of Bacterial and Fungal Organisms in Traditional Chinese Medicine

There has been an increase in use of Traditional Chinese Medicine (TCM) in the United States because they are less expensive and believed to be more effective with less adverse effects in comparison to traditional pharmaceutics. Therefore, sales have increased in the US, despite articles and case studies demonstrating the dangers, such as injury and death, related to TCM, stemming from improper labelling, toxic contaminants, and, in some cases, the presence of pathogenic bacteria. The aim of this study was to perform a survival experiment to demonstrate the importance of proper herbal brewing technique and to conduct a molecular and biochemical survey of microorganisms present on eleven Chinese herbal samples. The survival study compared Chinese brewing preparation and American brewing preparation by fortifying the herbal mixture with known bacteria and assessing its survival after brewing. The American brewed herbal tea was calculated to contain upwards of 3000 CFU (colony forming units)/mL, where the Chinese brewed herbal tea contained roughly 50 CFU/mL. FAME (Fatty Acid Methyl Ester) analysis was performed on the herbs to characterize any microorganisms present on the plant material already, following purchase. Strains within the Bacillus genus were identified in nearly all eleven of the herbal samples. These included B. subtilis and B. megaterium. Organisms belonging to the Bacillus ACT group (anthracis, cereus, thuringiensis) were identified in five out of eleven herb cultures as evidenced by the large ratio of 15:0 iso to 15:0 anteiso fatty acid biomarkers. Nine out of eleven herbal specimens also exhibited fungal biomarkers such as polyunsaturated 20:4 ω6,9,12,15c, and 18:3 ω6c (6,9,12).https://scholarscompass.vcu.edu/uresposters/1291/thumbnail.jp

Models that predict competitive employment outcomes in the United States Federal/State Vocational Rehabilitation program for clients who are blind and clients with other disabilities

The majority of clients in the Federal/State Vocational Rehabilitation program (VR) have been successful in achieving competitive employment in recent years. However, one disability group – clients who are legally blind – has traditionally lagged and currently lags behind in obtaining similar proportions of competitive employment outcomes as their counterparts in the VR system. In this study, the 2007 RSA 911 data were used to explore potential explanations for the discrepancy between outcomes for clients who are legally blind and clients with other disabilities. Similar to previous studies, frequency analyses confirmed that clients who are legally blind are far less likely to obtain a competitive employment outcome in the VR program. Although the most recent data reveals that 62.4% of clients who are legally blind attained a competitive outcome in 2007, 96.6% of clients with other disabilities achieved the same outcome. Backwards stepwise logistic regression generated two models yielding likelihoods of competitive employment for people who are blind and people with other disabilities, respectively. The model that predicts competitive closure for clients who are blind was generally similar to the model that predicts competitive closure for clients with other disabilities. Most of the service variables that predicted competitive outcomes for clients with other disabilities also predicted competitive outcomes for clients who are legally blind. However, the rates with which clients who are legally blind received these services were lower when compared to clients with other disabilities. One difference between the two models was that the variables predictive of competitive employment in both models often had larger odds ratios for clients who are legally blind. The models generated in this study will hopefully provide VR professionals with information that will contribute to helping clients who are legally blind achieve higher percentages of competitive employment outcomes

Evaluation of Two Commercially Available Cannabidiol Formulations for Use in Electronic Cigarettes

Since 24 states and the District of Columbia have legalized marijuana in some form, suppliers of legal marijuana have developed Cannabis sativa products for use in electronic cigarettes (e-cigarettes). Personal battery powered vaporizers, or e-cigarettes, were developed to deliver a nicotine vapor such that smokers could simulate smoking tobacco without the inherent pathology of inhaled tobacco smoke. The liquid formulations used in these devices are comprised of an active ingredient such as nicotine mixed with vegetable glycerin (VG) and/or propylene glycol (PG) and flavorings. A significant active ingredient of C. sativa, cannabidiol (CBD), has been purported to have anti-convulsant, anti-nociceptive, and anti-psychotic properties. These properties have potential medical therapies such as intervention of addictive behaviors, treatments for epilepsy, management of pain for cancer patients, and treatments for schizophrenia. However, CBD extracted from C. sativa remains a DEA Schedule I drug since it has not been approved by the FDA for medical purposes. Two commercially available e-cigarette liquid formulations reported to contain 3.3 mg/mL of CBD as the active ingredient were evaluated. These products are not regulated by the FDA in manufacturing or in labeling of the products and were found to contain 6.5 and 7.6 mg/mL of CBD in VG and PG with a variety of flavoring agents. Presently, while labeled as to content, the quality control of manufacturers and the relative safety of these products is uncertain

Powering lights with piezoelectric energy harvesting floors

The present work introduces a new technology for converting energy from steps into electricity. It starts with a study of the mechanical energy available from steps in a busy corridor. The subsequent development efforts and devices are presented, with an iterative approach to prototyping. Methods for enhancing the piezoelectric conversion efficiency have been determined as a part of the process and are introduced in the present article. Capitalizing on these findings, we have fabricated energy-harvesting devices for stairs that power embedded emergency lighting. The typical working unit comprises an energy-harvesting stair nosing, a power management circuit, and an embedded light-emitting diode that lights the tread in front of the user with an illuminance corresponding to emergency standards. The stair nosing generates up to 17.7 mJ of useful electrical energy per activation to provide up to 10.6 seconds of light. The corresponding energy density is 0.49 J per meter square and per step, with an 8.5 mm thick active layer

The Effect of Electronic Cigarette User Modifications and E-liquid Adulteration on the Particle Size Profile of an Aerosolized Product

Electronic cigarettes (e-cigarettes) are an alternate nicotine delivery system that generate a condensation aerosol to be inhaled by the user. The size of the droplets formed in the aerosol can vary and contributes to drug deposition and ultimate bioavailability in the lung. The growing popularity of e-cigarette products has caused an increase in internet sources promoting the use of drugs other than nicotine (DOTNs) in e-cigarettes. The purpose of this study was to determine the effect of various e-cigarette and e-liquid modifications, such as coil resistance, battery voltage, and glycol and drug formulation, on the aerosol particle size. E-liquids containing 12 mg/mL nicotine prepared in glycol compositions of 100% propylene glycol (PG), 100% vegetable glycerin (VG), or 50:50 PG:VG were aerosolized at three voltages and three coil resistances. Methamphetamine and methadone e-liquids were prepared at 60 mg/mL in 50:50 PG:VG and all e-liquids were aerosolized onto a 10 stage Micro-Orifice Uniform Deposit Impactor. Glycol deposition correlated with drug deposition, and the majority of particles centered between 0.172–0.5 μm in diameter, representing pulmonary deposition. The 100% PG e-liquid produced the largest aerosol particles and the 100% VG and 50:50 PG:VG e-liquids produced ultra-fine particles \u3c0.3 μm. The presence of ultrafine particles indicates that drugs can be aerosolized and reach the pulmonary alveolar regions, highlighting a potential for abuse and risk of overdose with DOTNs aerosolized in an e-cigarette system