8 research outputs found
The Vehicle, Fall 2010
Table of ContentsPoetryFill Your Mouth with BerriesAaron Whitepage 1
RelationsJamie Van Allenpage 2
ExodusMegan Marie Olsonpage 4
Single FileRashelle McNairpage 7
The Aesthetic Value of the Moon, by CandlelightKathy Deckerpage 15
FactalsGabrielle Keigherpage 16
Day 5David Jacksonpage 17
Esta LloviendoHeather Gerrishpage 19
FacebrokeDarrin Gordonpage 23
5:08 pmNikki Riechertpage 24
Train TunnelsAshton Tembypage 34
VariationsKathy Deckerpage 35
WantRashelle McNairpage 36
FriendshipScott Maypage 37
Golden LandJacob Swansonpage 38
Last Night I DreamtAshton Tembypage 39
Smallest GestureScott Maypage 44
Somebody\u27s Hut in MexicoGinamarie Lobiancopage 45
Some Things You Just Can\u27t Tap Dance AroundClint Walkerpage 53
Prose
Lamparus de DiosAaron Whitepage 8
Learning CurveScott Maypage 18
RocktonKatelyn Pfaffpage 20
Fatal DistractionSolomohn Ennispage 25
Noodle NonsenseGabrielle Keigherpage 41
AntarcticaMichael Payeapage 46
Special Features
James K Johnson Award Winners:
God is GraciousJohn Klyczekpage 57
To My Ever Growing ChestJennifer Hindespage 74
God\u27s ScapegoatJennifer Hindespage 76
Rape (Verb, Noun)Jennifer Hindespage 78
Featured Artist: Ashton Tembypage 81
Editor\u27s Pick: The Shooter by Patrick Hallpage 87
Chapbook 2010 Author:Kim Hunter-Perkinspage 114
About the Contributorspage 118
About the Editorspage 122https://thekeep.eiu.edu/vehicle/1092/thumbnail.jp
The Vehicle, Fall 2010
Table of ContentsPoetryFill Your Mouth with BerriesAaron Whitepage 1
RelationsJamie Van Allenpage 2
ExodusMegan Marie Olsonpage 4
Single FileRashelle McNairpage 7
The Aesthetic Value of the Moon, by CandlelightKathy Deckerpage 15
FactalsGabrielle Keigherpage 16
Day 5David Jacksonpage 17
Esta LloviendoHeather Gerrishpage 19
FacebrokeDarrin Gordonpage 23
5:08 pmNikki Riechertpage 24
Train TunnelsAshton Tembypage 34
VariationsKathy Deckerpage 35
WantRashelle McNairpage 36
FriendshipScott Maypage 37
Golden LandJacob Swansonpage 38
Last Night I DreamtAshton Tembypage 39
Smallest GestureScott Maypage 44
Somebody\u27s Hut in MexicoGinamarie Lobiancopage 45
Some Things You Just Can\u27t Tap Dance AroundClint Walkerpage 53
Prose
Lamparus de DiosAaron Whitepage 8
Learning CurveScott Maypage 18
RocktonKatelyn Pfaffpage 20
Fatal DistractionSolomohn Ennispage 25
Noodle NonsenseGabrielle Keigherpage 41
AntarcticaMichael Payeapage 46
Special Features
James K Johnson Award Winners:
God is GraciousJohn Klyczekpage 57
To My Ever Growing ChestJennifer Hindespage 74
God\u27s ScapegoatJennifer Hindespage 76
Rape (Verb, Noun)Jennifer Hindespage 78
Featured Artist: Ashton Tembypage 81
Editor\u27s Pick: The Shooter by Patrick Hallpage 87
Chapbook 2010 Author:Kim Hunter-Perkinspage 114
About the Contributorspage 118
About the Editorspage 122https://thekeep.eiu.edu/vehicle/1092/thumbnail.jp
MassIVE MSV000091077 - Senescence-associated dysregulation of translation inhibits activation of the integrated stress response
MassIVE MSV000091116 - Senescence-specific translation dysregulation desensitizes cells to stress by inhibiting activation of the integrated stress response
Extracellular gluco-oligosaccharide degradation by Caulobacter crescentus
The oligotrophic bacterium Caulobacter crescentus has the ability to metabolize various organic molecules, including plant structural carbohydrates, as a carbon source. The nature of β-glucosidase (BGL)-mediated gluco-oligosaccharide degradation and nutrient transport across the outer membrane in C. crescentus was investigated. All gluco-oligosaccharides tested (up to celloheptose) supported growth in M2 minimal media but not cellulose or CM-cellulose. The periplasmic and outer membrane fractions showed highest BGL activity, but no significant BGL activity was observed in the cytosol or extracellular medium. Cells grown in cellobiose showed expression of specific BGLs and TonB-dependent receptors (TBDRs). Carbonyl cyanide 3-chlorophenylhydrazone lowered the rate of cell growth in cellobiose but not in glucose, indicating potential cellobiose transport into the cell by a proton motive force-dependent process, such as TBDR-dependent transport, and facilitated diffusion of glucose across the outer membrane via specific porins. These results suggest that C. crescentus acquires carbon from cellulose-derived gluco-oligosaccharides found in the environment by extracellular and periplasmic BGL activity and TBDR-mediated transport. This report on extracellular degradation of gluco-oligosaccharides and methods of nutrient acquisition by C. crescentus supports a broader suite of carbohydrate metabolic capabilities suggested by the C. crescentus genome sequence that until now have not been reported
Mutations in the anticodon stem of tRNA cause accumulation and Met22-dependent decay of pre-tRNA in yeast
Identification of the determinants of tRNA function and susceptibility to rapid tRNA decay by high-throughput in vivo analysis
Extracellular gluco-oligosaccharide degradation by Caulobacter crescentus
The oligotrophic bacterium Caulobacter crescentus has the ability to metabolize various organic molecules, including plant structural carbohydrates, as a carbon source. The nature of β-glucosidase (BGL)-mediated gluco-oligosaccharide degradation and nutrient transport across the outer membrane in C. crescentus was investigated. All gluco-oligosaccharides tested (up to celloheptose) supported growth in M2 minimal media but not cellulose or CM-cellulose. The periplasmic and outer membrane fractions showed highest BGL activity, but no significant BGL activity was observed in the cytosol or extracellular medium. Cells grown in cellobiose showed expression of specific BGLs and TonB-dependent receptors (TBDRs). Carbonyl cyanide 3-chlorophenylhydrazone lowered the rate of cell growth in cellobiose but not in glucose, indicating potential cellobiose transport into the cell by a proton motive force-dependent process, such as TBDR-dependent transport, and facilitated diffusion of glucose across the outer membrane via specific porins. These results suggest that C. crescentus acquires carbon from cellulose-derived gluco-oligosaccharides found in the environment by extracellular and periplasmic BGL activity and TBDR-mediated transport. This report on extracellular degradation of gluco-oligosaccharides and methods of nutrient acquisition by C. crescentus supports a broader suite of carbohydrate metabolic capabilities suggested by the C. crescentus genome sequence that until now have not been reported