The influence of high temperature on the possibility of DNA typing in various human tissues

Introduction. The identification of unknown victims of high temperatures (fire, terrorist attack, and other disasters) is one of the most difficult tasks faced by forensic geneticists. The main aim of this study was to in­vestigate the availability of DNA isolated from various human tissue samples exposed to high temperatures of 100–1000°C for 5 and 10 minutes. Material and methods. Samples of varying thickness of thigh muscle, liver, heart, adipose tissue, bone, teeth, hair and nails of 52 fresh cadavers and 59 healthy teeth of 29 volunteers were used. The study was performed using the following commercially available STR (Short Tandem Repeats) and miniSTR kits: AmpFlSTR®SGM Plus® and AmpFlSTR®MiniFilerTM. Hyper variable region I (HVI) of human mitochondrial DNA (mtDNA) was sequenced with BigDye Terminator Cycle Sequencing Kit 1.1. The PEP (Primer-Extension Preamplification) method was used for the whole human genome amplification. Results. It was possible to obtain complete DNA profiles (AmpFlSTR®SGM Plus®, AmpFlSTR®MiniFilerTM Applied Biosystems, USA and mtDNA HVI region) for tissue samples of heart, liver and thigh muscle, exposed up to 900°C for 5 min. However, under the applied conditions, limited usefulness of hair, nails and teeth for identification purposes was shown. Conclusions. DNA stability in tissues subjected to incineration depends on many factors, like tissue type and its thickness, temperature and time of exposure. In the cases of human remains exposed to high temperatures, samples of soft tissues of the highest weight (thickness) provide the best chance of successful identification through the genetic analysis. In some cases of negative results, even if using mtDNA typing, application of the whole genome amplification (WGA) technique could provide the expected results for highly degraded DNA templates

Changes in habitat conditions in a Late Glacial fluviogenic lake in response to climatic fluctuations (Warta River valley, central Poland)

The Warta River val ley was greatly in flu enced by the ice sheet of the Last Gla cial Max i mum (LGM). A small peatland lo cated in the Warta drain age sys tem is here used as a palaeoarchive of cli ma tic and hab i tat changes dur ing the Late Gla cial (Weichselian). The ugi pound sed i ment pro file was in ves ti gated us ing multi-proxy (pol len, Chironomidae, Cladocera and geo chem is try) anal y ses that re corded changes in a fluviogenic sed i men tary de pres sion. Af ter the Poznan Phase (LGM), ugi pound func tioned as an ox bow lake that was cut off from the ac tive river chan nel as a re sult of flu vial ero sion. Since that time, the Warta River has flowed only along the sec tion now oc cu pied by the Jeziorsko Res er voir. Sed i men ta tion of lac us trine de pos its started at the be gin ning of the Late Gla cial. Sum mer tem per a ture re con struc tions in di cate cool Old est and Youn ger Dryas, but no clear cool ing in the Older Dryas. Dur ing the Youn ger Dryas the palaeolake was com pletely oc cu pied by a peatland (fen), which pe ri od i cally dried out dur ing the Ho lo cene. In ves ti ga tion of this site has tracked the re ac tion of the hab i tat to cli ma tic, hy dro log i cal and geomorphological changes through out the Late Weichselian.Peer reviewe