The Effect of Organizational Climate on Presenteeism as Moderated by Employee Personality

This research study was an investigation of the relationship between the five dimensions of personality, organizational climate, and presenteeism - coming to work when ill. Presenteeism has significant, negative impact on employee’s long-term health, the overall organizational performance, and more broadly on national health systems. Researchers have found that in addition to financial impacts of not working while ill, antecedents of presenteeism include both personal, situational (e.g., the severity of the illness), and organizational circumstances. A systematic exploration into all five dimensions of personality and organizational climate has not been conducted to date. The purpose of this quantitative study was to examine the relationship between personality, as measured by the Big Five Inventory, organizational climate, as measured by the Organizational Climate Measure, and presenteeism. A survey was used to gather primary data from employees working in the UK and the Netherlands (N = 88). Logistic regression determined that none of expected dimensions of personality (neuroticism, conscientiousness, and extroversion) were correlated with presenteeism and, of the eight organizational climate measures, only innovation and flexibility was positively correlated with presenteeism, and integration was negatively related. Although the overall regression models failed to demonstrate any predictive relationships between personality, organizational climate, and presenteeism that were anticipated, the study provides new insights for organizational leaders on how certain aspects of organizational climate impacts employee behaviors when ill, facilitating positive social change by ensuring organizational interventions are designed with employee wellbeing in mind

Role of echocardiography in stress cardiomyopathy diagnosis after tracheal extubation

Stress cardiomyopathy or Takotsubo cardiomyopathy is a new syndrome and still insufficiently recognized among emergency patients, hospitalized patients. Many different physical and emotional stressors are triggers, but individual susceptibility to Takotsubo cardiomyopathy can not be predicted. Takotsubo cardiomyopathy in surgical and critical care population is a huge diagnostic challenge. Apart that these patients are treated in stressful environment and conditions. Postoperatively due to change of consciousness and inability to speak we can not rely on verbal symptoms to make differential diagnosis. Although essential sometimes they can not be submitted to coronary angiography to exclude obstructive coronary disease due many risk factors. So, then we follow clinical course, electrocardiographic, radiologic and echocardiographic dynamic changes, laboratory findings and consiliar opinion to make diagnosis. We represent a case of Takotsubo cardiomyopathy in a 59-years old postmenopausal Caucasian woman after tracheal extubation. She was submitted to surgery of intraabdominal collection evacuation in short general endotracheal anesthesia

Spinal cord injury without radiological abnormality (SCIWORA) in a young female and pharmacological treatment option: a case report with review of literature

Acute spinal cord injury strikes labour active young and middle-aged population, especially men, and consequently usually results in difficult neurological sequel. Also disables normal quality of life and everyday functioning in these patients despite many available supportive measures. Spinal cord injury without radiological abnormality (SCIWORA) presents a great diagnostic challenge because radiological and computed tomography pictures are without visible pathology which would explain the new onset of the neurological deficit. For the first time we report a true spinal cord injury without radiological abnormality in the X-ray, computed tomography and magnetic resonance imaging in a young female manifested with moderate neurological deficit after the traffic accident. Although SCIWORA is very rare in adults, high level of suspicion in emergency department is advisable as the timely applied neuroprotective measures can prevent the onset of the neurological deficit. It is important to emphasize that emergency magnetic resonance imaging application is the diagnostic key. Albeit, the SCIWORA neurologic deficit can show up with a delay of four days after the spinal cord injury in a patient with a normal physical and radiological examination. Treatment of these patients is another challenge for every physician. In fact pharmacological treatment options are still in the experimental research phase. We can conclude that currently the neuroprotective measures of the acute spinal cord injury patient started in the emergency department regardless the radiological test findings represent the right and successful key treatment.

High-Dimensional Single-Cell Mapping of Central Nervous System Immune Cells Reveals Distinct Myeloid Subsets in Health, Aging, and Disease

Individual reports suggest that the central nervous system (CNS) contains multiple immune cell types with diverse roles in tissue homeostasis, immune defense, and neurological diseases. It has been challenging to map leukocytes across the entire brain, and in particular in pathology, where phenotypic changes and influx of blood-derived cells prevent a clear distinction between reactive leukocyte populations. Here, we applied high-dimensional single-cell mass and fluorescence cytometry, in parallel with genetic fate mapping systems, to identify, locate, and characterize multiple distinct immune populations within the mammalian CNS. Using this approach, we revealed that microglia, several subsets of border-associated macrophages and dendritic cells coexist in the CNS at steady state and exhibit disease-specific transformations in the immune microenvironment during aging and in models of Alzheimer's disease and multiple sclerosis. Together, these data and the described framework provide a resource for the study of disease mechanisms, potential biomarkers, and therapeutic targets in CNS disease

New PEO-IAA-Inspired Anti-Auxins: Synthesis, Biological Activity, and Possible Application in Hemp (Cannabis Sativa L.) Micropropagation

Auxins play an important role in plant physiology and are involved in numerous aspects of plant development, such as cell division, elongation and differentiation, fruit development, and phototropic response. In addition, through their antagonistic interaction with cytokinins, auxins play a key role in the regulation of root growth and apical dominance. Thanks to this capacity to determine plant architecture, natural and synthetic auxins have been successfully employed to obtain more economically advantageous plants. The crosstalk between auxins and cytokinins determines plant development and thus is of particular importance in the field of plant micropropagation, where the ratios between these two phytohormones need to be tightly controlled to achieve proper rooting and shoot generation. Previously reported anti-auxin PEO-IAA, which blocks auxin signalling through binding to TIR1 receptor and inhibiting the expression of auxin-responsive genes, has been successfully used to facilitate hemp micropropagation. Herein, we report a set of new PEO-IAA-inspired anti-auxins capable of antagonizing auxin responses in vivo. The capacity of these compounds to bind to the TIR1 receptor was confirmed in vitro by SPR analysis. Using DESI-MSI analysis, we evaluated the uptake and distribution of the compounds at the whole plant level. Finally, we characterized the effect of the compounds on the organogenesis of hemp explants, where they showed to be able to improve beneficial morphological traits, such as the balanced growth of all the produced shoots and enhanced bud proliferation

Intrapartal resection of the double cervix and longitudinal vaginal septum after hysteroscopic resection of the complete uterine septum, resulting in a term vaginal delivery: A case report

Objective: To report a rare uterine anomaly of a septate uterus, double cervix and double vagina in patient who conceived spontaneously and delivered vaginaly. Design: Case report. Setting: Department of Obstetrics and Gynecology, Zagreb University School of Medicine, Clinical Hospital “Sveti Duh”, Zagreb, Croatia. Patient(s): A 34-year-old nulligravida who underwent clinical, radiological, surgical and intrapartal workup. Intervention(s): Clinical examination and intrapartal surgical resection of vaginal septum followed by vaginal delivery. Main Outcome Measure(s): Description and treatment for a rare Müllerian anomaly and a subsequent literature search. Result(s): Successful intrapartal resection of longitudinal vaginal septum and double cervix followed by vaginal delivery without complication. Conclusion(s): Reconstructive surgical procedures may be considered for providing spontaneous pregnancies and intrapartal surgical technique could be taken into consideration in order to prevent unnecessary cesarean sections, upon strict estimation of the obstetrician

Molecular genetic strategy for diagnosis of congenital adrenal hyperplasia in Serbia

Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency is one of the most common endocrine diseases, yet genetic diagnosis is among the most complicated of all monogenic disorders. It has an overall incidence of 1: 10000-1: 20000, it is inherited in autosomal recessive pattern and caused by mutations affecting CYP21A2 gene. Based on the phenotypic expression, this disease is categorized into severe, classical form revealed at birth and mild, non-classical form. Although diagnosis could be established based on biochemical tests and distinctive clinical features, molecular genetic testing is crucial for diagnosis confirmation, detection of carriers and asymptomatic patients, disease prognosis, as well as for providing proper genetic counselling and prenatal diagnosis. Based on CYP21A2 mutational spectrum and frequencies in Serbia, in this paper we propose an optimal molecular genetic diagnostic algorithm for CAH and discuss genetic mechanisms underlying the disease. The complete diagnostic procedure combines multiplex minisequencing technique (SNaPshot PCR) as a method for rapid detection of common point mutations, direct sequencing of whole CYP21A2 gene and PCR with sequence specific primers (PCR-SSP) for large gene rearrangements detection (CYP21A1P/CYP21A2 chimeras). While SNaPshot PCR assay analyses ten common mutations (c. 290-13A/C gt G, p.P30L, p.R356W, p.G110fs, p.V281L, p.Q318X, p.L307fs, p.I172N, Cluster p.[I236N;V237E;M239K] and p.P453S) which account for over 80% of all CYP21A2 mutations in Serbian population, direct sequencing of CYP21A2 gene is needed to identify potential rare or novel mutations present in Serbian population with frequency of 1.8%. Additionally, large gene rearrangements which are present with frequency of 16.7% make PCR-SSP analysis an unavoidable part of molecular characterization of CAH in Serbia. Described molecular genetic strategy is intended to facilitate correct diagnosis assessment in CAH affected individuals and their families in Serbia but it will also contribute to molecular genetic testing of CAH patients across Europe