Hyalesthes obsoletus, a vector of stolbur phytoplasma: current situation in South Moravia, Czech Republic

Abstract A survey for the presence of Hyalestes obsoletus Signoret in 2010 confirmed a dramatic increase in number of captured individuals at observed localities in agriculturally important areas in southern Moravia, Czech Republic. Population density from the end of June to the middle of July, varies with locality. A preferred host plant Urtica dioica L. was present. Average percentage of stolbur phytoplasma positive H. obsoletus specimens varied from 31 to 59%

Tuf and secY PCR amplification and genotyping of phytoplasmas

Tuf and secY genotyping techniques have been developed to distinguish phytoplasma strains. Tuf polymerase chain reaction sequence analyses are available for phytoplasma taxonomic groups 16SrI, 16SrV, 16SrXII-A, and XII-B. In addition to their use to confirm the taxonomic status of phytoplasma strains, they allow the spread of phytoplasma strains in host plants and insect vectors to be traced. SecY is more variable than tuf and is therefore more discriminatory than tuf, but secY and tuf phylogenies show congruence

Rozvíjení interkulturní komunikační kompetence ve výuce cizích jazyků

centrem, o.p.s