Size-independent Young's modulus of inverted conical GaAs nanowire resonators

We explore mechanical properties of top down fabricated, singly clamped inverted conical GaAs nanowires. Combining nanowire lengths of 2-9 $\mu$m with foot diameters of 36-935 nm yields fundamental flexural eigenmodes spanning two orders of magnitude from 200 kHz to 42 MHz. We extract a size-independent value of Young's modulus of (45$\pm$3) GPa. With foot diameters down to a few tens of nanometers, the investigated nanowires are promising candidates for ultra-flexible and ultra-sensitive nanomechanical devices

Collective dynamics of strain-coupled nanomechanical pillar resonators

Semiconductur nano- and micropillars represent a promising platform for hybrid nanodevices. Their ability to couple to a broad variety of nanomechanical, acoustic, charge, spin, excitonic, polaritonic, or electromagnetic excitations is utilized in fields as diverse as force sensing or optoelectronics. In order to fully exploit the potential of these versatile systems e.g. for metamaterials, synchronization or topologically protected devices an intrinsic coupling mechanism between individual pillars needs to be established. This can be accomplished by taking advantage of the strain field induced by the flexural modes of the pillars. Here, we demonstrate strain-induced, strong coupling between two adjacent nanomechanical pillar resonators. Both mode hybridization and the formation of an avoided level crossing in the response of the nanopillar pair are experimentally observed. The described coupling mechanism is readily scalable, enabling hybrid nanomechanical resonator networks for the investigation of a broad range of collective dynamical phenomena

Cavity cooling of a nanomechanical resonator by light scattering

We present a novel method for opto-mechanical cooling of sub-wavelength sized nanomechanical resonators. Our scheme uses a high finesse Fabry-Perot cavity of small mode volume, within which the nanoresonator is acting as a position-dependant perturbation by scattering. In return, the back-action induced by the cavity affects the nanoresonator dynamics and can cool its fluctuations. We investigate such cavity cooling by scattering for a nanorod structure and predict that ground-state cooling is within reach.Comment: 4 pages, 3 figure

Fluctuating nanomechanical system in a high finesse optical microcavity

The idea of extending cavity quantum electrodynamics experiments to sub-wavelength sized nanomechanical systems has been recently proposed in the context of optical cavity cooling and optomechanics of deformable cavities. Here we present an experiment involving a single nanorod consisting of about 10$^{9}$ atoms precisely positioned into the confined mode of a miniature high finesse Fabry-Pérot microcavity. We show that the optical transmission of the cavity is affected not only by the static position of the nanorod but also by its vibrational fluctuation. The Brownian motion of the nanorod is resolved with a displacement sensitivity of 200 fm/√Hz at room temperature. Besides a broad range of sensing applications, cavity-induced manipulation of optomechanical nanosystems and back-action is anticipated

Life Cycle-Dependent Cytoskeletal Modifications in Plasmodium falciparum Infected Erythrocytes

10.1371/journal.pone.0061170PLoS ONE84

A Rigidity-Enhanced Antimicrobial Activity: A Case for Linear Cationic α-Helical Peptide HP(2–20) and Its Four Analogues

Linear cationic α-helical antimicrobial peptides are referred to as one of the most likely substitutes for common antibiotics, due to their relatively simple structures (≤40 residues) and various antimicrobial activities against a wide range of pathogens. Of those, HP(2–20) was isolated from Helicobacter pylori ribosomal protein. To reveal a mechanical determinant that may mediate the antimicrobial activities, we examined the mechanical properties and structural stabilities of HP(2–20) and its four analogues of same chain length by steered molecular dynamics simulation. The results indicated the following: the resistance of H-bonds to the tensile extension mediated the early extensive stage; with the loss of H-bonds, the tensile force was dispensed to prompt the conformational phase transition; and Young's moduli (N/m2) of the peptides were about 4∼8×109. These mechanical features were sensitive to the variation of the residue compositions. Furthermore, we found that the antimicrobial activity is rigidity-enhanced, that is, a harder peptide has stronger antimicrobial activity. It suggests that the molecular spring constant may be used to seek a new structure-activity relationship for different α-helical peptide groups. This exciting result was reasonably explained by a possible mechanical mechanism that regulates both the membrane pore formation and the peptide insertion

Comparative proteomic analysis of metabolically labelled proteins from Plasmodium falciparum isolates with different adhesion properties

The virulence of Plasmodium falciparum relates in part to the cytoadhesion characteristics of parasitized erythrocytes but the molecular basis of the different qualitative and quantitative binding phenotypes is incompletely understood. This paucity of information is due partly to the difficulty in working with membrane proteins, the variant nature of these surface antigens and their relatively low abundance. To address this two-dimensional (2D) protein profiles of closely related, but phenotypically different laboratory strains of P. falciparum have been characterized using proteomic approaches. Since the mature erythrocyte has no nucleus and no protein synthesis capability, metabolic labelling of proteins was used to selectively identify parasite proteins and increase detection sensitivity. A small number of changes (less than 10) were observed between four different P. falciparum laboratory strains with distinctive cytoadherence properties using metabolic labelling, with more parasite protein changes found in trophozoite iRBCs than ring stage. The combination of metabolic labelling and autoradiography can therefore be used to identify parasite protein differences, including quantitative ones, and in some cases to obtain protein identifications by mass spectrometry. The results support the suggestion that the membrane protein profile may be related to cytoadherent properties of the iRBCs. Most changes between parasite variants were differences in iso-electric point indicating differential protein modification rather than the presence or absence of a specific peptide

Curing advanced melanoma by 2025

PURPOSE OF REVIEW To outline the most urgent challenges in the management of advanced melanoma. RECENT FINDINGS Considerable progress in targeted and immunotherapy of advanced melanoma has opened a perspective for a cure if all molecular and medical information is integrated in a rational precision treatment algorithm. SUMMARY Bioinformatics and system biology approaches will be needed to deal with omics databases. The support of patient advocacy groups may help to increase the acceptance of large scale, routine biobanking