Effects of intracameral brilliant blue on the corneal endothelium of swine: in vitro study

Abstract: The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue±) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05% brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery

Homologous pericardium preserved in glycerin used to repair ocular perforation in a dog

As lesões de córnea ocorrem frequentemente em cães. Várias técnicas cirúrgicas são propostas para reparar perfuração de córnea, incluindo flapes conjuntivais, ceratoplastias, enxertos corneanos e implantes biológicos. Um caso de perfuração ocular em um cão tratado com pericárdio homólogo conservado em glicerina é apresentado. Oito anos de idade, macho, Pit Bull foi encaminhado ao Serviço de Oftalmologia do Hospital de Clínicas Veterinárias da Universidade Federal do Rio Grande do Sul (UFRGS) com 12 horas de perfuração corneana. O exame oftálmico revelou desconforto ocular, epífora, hiperemia conjuntival e perfuração corneana central com 5mm no olho direito. A correção cirúrgica foi realizada utilizando pericárdio homólogo conservado em glicerina. O uso de pericárdio homólogo conservado em glicerina foi efetivo na reparação do defeito corneano, preservando as funções e a anatomia oculares.The corneal lesions occur frequently in dogs. Many surgical techniques have been proposed for repair of corneal perforation including conjunctival flaps, keratoplastic procedures, xenografts and biologicals grafts. A case report of ocular perforation in a dog treated sucessfully with homologous pericardium preserved in glycerin is presented. An eight year-old, male, Pit Bull, was presented to the Ophthalmology Service of the Hospital de Clínicas Veterinárias of the Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil with a 12 hour of a corneal perforation. The ophthalmic examination revealed ocular disconfort, epiphora, conjunctival hyperaemia and a central corneal perforation measuring 5mm in the right eye. Surgical repair was performed using homologous pericardium preserved in glycerin. Use of the homologous pericardium preserved in glycerin was effective for repairing corneal defect, preserving oculars function and anatomy

Homologous pericardium preserved in glycerin used to repair ocular perforation in a dog

As lesões de córnea ocorrem frequentemente em cães. Várias técnicas cirúrgicas são propostas para reparar perfuração de córnea, incluindo flapes conjuntivais, ceratoplastias, enxertos corneanos e implantes biológicos. Um caso de perfuração ocular em um cão tratado com pericárdio homólogo conservado em glicerina é apresentado. Oito anos de idade, macho, Pit Bull foi encaminhado ao Serviço de Oftalmologia do Hospital de Clínicas Veterinárias da Universidade Federal do Rio Grande do Sul (UFRGS) com 12 horas de perfuração corneana. O exame oftálmico revelou desconforto ocular, epífora, hiperemia conjuntival e perfuração corneana central com 5mm no olho direito. A correção cirúrgica foi realizada utilizando pericárdio homólogo conservado em glicerina. O uso de pericárdio homólogo conservado em glicerina foi efetivo na reparação do defeito corneano, preservando as funções e a anatomia oculares.The corneal lesions occur frequently in dogs. Many surgical techniques have been proposed for repair of corneal perforation including conjunctival flaps, keratoplastic procedures, xenografts and biologicals grafts. A case report of ocular perforation in a dog treated sucessfully with homologous pericardium preserved in glycerin is presented. An eight year-old, male, Pit Bull, was presented to the Ophthalmology Service of the Hospital de Clínicas Veterinárias of the Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil with a 12 hour of a corneal perforation. The ophthalmic examination revealed ocular disconfort, epiphora, conjunctival hyperaemia and a central corneal perforation measuring 5mm in the right eye. Surgical repair was performed using homologous pericardium preserved in glycerin. Use of the homologous pericardium preserved in glycerin was effective for repairing corneal defect, preserving oculars function and anatomy