Antioxidant and anitoperoxidative effect of polypeptides from common beans (Phaseolus vulgaris, cv BRS Pontal) / Efeito antioxidante e anitoperoxidativo dos polipéptidos do feijão comum (Phaseolus vulgaris, cv BRS Pontal)

Common beans have high content of protein and can be used as source for obtaining bioactive peptides which are compounds that exhibit an effect on body functions or conditions and may influence human health. This study was undertaken to examine the antioxidant and antiperoxidative potential of naturally-occurring peptides from easy-to-cook (ETC) and hard-to-cook (HTC) beans (Phaseolus vulgaris, cv BRS Pontal). The extracted proteins were partially purified using ultrafiltration membranes. Thereafter, the antioxidant activity of the produced fractions was analyzed by DPPH and FRAP methods. The lipid peroxidation was measured by thiobarbituric acid reactive substances (TBARS) analysis. Results evidenced that the antioxidant potential was highly improved after ultrafiltration, especially for those fractions containing molecules with low molecular weight. Peptide fractions (F3-10kDa) from ETC beans exhibited a better ability to inhibit the reactive oxygen species formation when compared to the synthetic antioxidant BHT, which corroborates their antioxidant role and protection against lipid peroxidation. Regarding the peptide fractions from HTC beans, the samples had similar lipid peroxidation to BHT in the tested concentration range (200 to 600?g). The HTC phenomenon did not seem to affect definitively the bioactivity of the bioactive peptides, suggesting that it is possible to use these components as an alternative for the use of the grains affected by the hardening process

Effect of different conservation methods on the bioactive compounds and antioxidant activity of mangaba (Hancornia speciosa Gomes)

Submitted by JÚLIO HEBER SILVA ([email protected]) on 2017-08-28T19:41:30Z No. of bitstreams: 2 Dissertação - Ladyslene Christhyns de Paula - 2015.pdf: 1525732 bytes, checksum: 4fcbdced13934388c78559440d071220 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2017-09-15T13:00:36Z (GMT) No. of bitstreams: 2 Dissertação - Ladyslene Christhyns de Paula - 2015.pdf: 1525732 bytes, checksum: 4fcbdced13934388c78559440d071220 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2017-09-15T13:00:36Z (GMT). No. of bitstreams: 2 Dissertação - Ladyslene Christhyns de Paula - 2015.pdf: 1525732 bytes, checksum: 4fcbdced13934388c78559440d071220 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2015-02-23Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESBrazil is a country of great diversity in species of native fruits, yet little explored, but with huge agro-industrial and nutritional potential, being mangaba one of these promising fruit. Research indicates that the intake of conventional, exotic and native fruits provide not only essential nutrients, but also bioactive compounds that promote beneficial effects on human health, reducing the risk of chronic diseases and even cancer, due to the interaction between these compounds and their antioxidant potential. This study aimed to evaluate the behavior of bioactive compounds and antioxidant activity in raw fruits mangaba (Hancornia speciosa Gomes), and the influence of conservation methods: freezing, freeze-drying and foam layer before these biocompounds. The samples were evaluated at Chemistry and Biochemistry Laboratory of Food of Faculty of Pharmacy of Federal University of Goiás, as regard of: ascorbic acid, phenolic compounds, carotenoids and total antioxidant activity by DPPH methods, ABTS and FRAP. Analyses were performed in triplicate for each sample, and the results were expressed as mean ± standard deviation. The results showed significant correlation between the content of total phenolic compounds and total antioxidant activity by FRAP method, showing that ascorbic acid content, contributed to the antioxidant activity of the samples. Thus, there is a possibility of using mangaba, both for consumption and fresh using, referred to as different methods of preservation, possibly enabling the use of the fruit as a functional food.O Brasil é um país com grande diversidade em espécies de frutas nativas, ainda pouco exploradas, mas com enorme potencial agroindustrial e nutricional, sendo a mangaba, uma destas frutíferas promissoras. Pesquisas apontam que a ingestão de frutas convencionais, exóticas e nativas fornecem não somente os nutrientes essenciais, mas também, compostos bioativos que promovem efeitos benéficos à saúde humana, reduzindo o risco de doenças crônicas não transmissíveis e até mesmo o câncer, devido à interação entre estes compostos e seu potencial antioxidante. Este estudo teve como proposta, avaliar o comportamento dos compostos bioativos e da atividade antioxidante em frutos in natura de mangaba (Hancornia speciosa Gomes), bem como a influência dos métodos de conservação: congelamento, liofilização, secagem em camada de espuma, frente a estes biocompostos. As amostras foram avaliadas no Laboratório de Química e Bioquímica de Alimentos, da Faculdade de Farmácia, da Universidade Federal de Goiás, quanto ao teor de: ácido ascórbico, compostos fenólicos totais, carotenoides e atividade antioxidante total pelos métodos DPPH, ABTS e FRAP. As análises foram realizadas em triplicata, para cada amostra, e os resultados expressos em média ± desvio padrão. Os resultados demonstraram correlação significativa entre o conteúdo de compostos fenólicos totais e a atividade antioxidante total pelo método do FRAP, dem onstrando que o teor de ácido ascórbico, contribuiu para a atividade antioxidante das amostras analisadas. Assim, verifica-se a possibilidade do uso da mangaba, tanto para o consumo na forma in natura, quanto submetido aos diferentes métodos de conservação, possibilitando possivelmente a utilização deste fruto como alimento funcional