Identifikasi Nilai-Nilai Cerita Wayang Beber Pacitan sebagai Media Pembelajaran Sejarah

Wayang Beber Pacitan merupakan hasil kearifan lokal atau local genius bangsa Indonesia dan menjadi local identity atau identitas budaya bangsa. Isinya mengenai kepahlawanan dan percintaan yang berpusat pada dua tokoh utama, yaitu raden Inu Kertapati atau Panji Asmarabangun, dan Dewi Sekartaji atau Galuh Candrakirana. Cerita dalam Wayang Beber Pacitan mempunyai folklor yang sarat dengan nilai-nilai simbolis yang masih relevan dengan masa sekarang. Penyebarluasan cerita Wayang Beber ini sangat penting untuk menjaga agar tidak punah dan mengajarkannya kepada generasi muda merupakan cara yang tepat. Salah satu media yang tepat adalah melalui Pembelajaran Sejarah.Pembelajaran sejarah haruslah mengembangkan tugas sebagai wahana “transmission of culture”. Fungsi didaktis Pembelajaran Sejarah dimana pengetahuan sejarah dimaksudkan agar generasi penerus bisa mengambil hikmah dari masa lalu selain itu juga mengambil pelajarannya. Sejarah sebagai sarana edukatif dan inspiratif memberikan nilai-nilai pendidikan bagi peserta didik yang sangat berguna dalam memberikan inspirasi kepada peserta didik. Nilai-nilai dalam cerita Wayang beber Pacitan dapat digunakan sebagai media Pembelajaran Sejarah, untuk memberikan pengalaman nyata yang dapat menumbuhkan kegiatan berusaha sendiri peserta didik. Oleh karena itu tulisan ini berusaha menguraikan nilai-nilai yang terkandung dalam cerita Wayang Beber sebagi media pembelajaran sejarah. Penulisan ini menggunakan metode deskriptif kualitatif

Acetyl-CoA appears to be a central hub connecting diverse pathways upregulated in Summer crowns and rhizomes.

<p>Cyan squares are KEGG pathways found to be upregulated in Summer plants by GSEA, and cyan circles are, and metabolites that were elevated in Summer plants relative to Kanlow plants. Edges connecting pathways (squares) to metabolites (circle) indicate that the given metabolite is found in the connected pathway. Pathways with direct connections in KEGG are also connected by edges. Acetyl-CoA (diamond) could be a potential linker molecule among these diverse pathways and is suggested to be a possible metabolic hub in Summer crowns and rhizomes entering dormancy.</p

Metabolite profiling reveals ecotype specific differences in crown and rhizome tissues.

<p>(<b>A</b>) PCA of overall metabolite profiles observed by GCMS for for each of three individual genotypes within each cultivar. Kanlow tissue extracts (orange triangles) are separated from Summer tissue extracts (cyan squares) by the first component. Two-way error bars are shown for each plant that was based on nine separate GCMS runs for each sample. (<b>B</b>) Volcano plot showing the log₁₀FDR versus the log_1.75 fold change in peak area for major ions for all metabolites detected by GCMS. Significant differences were observed for several metabolites between the two cultivars (Kanlow orange triangles) and Summer (cyan squares). Grey circles are metabolites that failed to show sufficient differences in ion area of had an FDR value of >0.05. (<b>C</b>) Heat map shows marked differences in tissue abundances of selected metabolites in Kanlow and Summer crowns and rhizomes. Data are the average of triplicate injections from each of three separate extractions from the three biological replicates of each cultivar. Scale is from high abundance (yellow) to low abundance (black) for each metabolite. Data were subjected to one-way hierarchical clustering based on cultivar, and metabolites were manually reordered into more biologically related groupings (A–G).</p

Transcripts for enzymes and several metabolites associated with the phenylpropanoid pathway are significantly enhanced in Kanlow crowns and rhizomes.

<p>Orange boxes identify individual enzymes upregulated in Kanlow. Boxes with EC numbers and relative enrichment of transcripts (Kanlow/Summer, K/S) are shown. Boxes with numbers indicate metabolites associated with this pathway identified by GCMS. Cyan (higher levels in Summer tissues), orange (higher in Kanlow tissues) and grey boxes (not significantly different).</p

Additional file 3: of Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs

Data S1 GO enrichment results (XLSX 12 kb

Additional file 1: Table S1. of Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs

Mapping statistics for RNA Seq (XLSX 12 kb

Additional file 2: Table S2. of Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs

Gene identities and primers used for RNA-Seq validation (XLSX 11 kb