TB20: Preliminary Tables of Some Chemical Elements in Seven Tree Species in Maine

These tables show the amount in grams for each of twelve elements for the complete tree and the merchantable bole, for seven tree species (red spruce, balsam fir, hemlock, white pine, white birch, red maple, aspen) in terms of five height classes and ten diameter classes.https://digitalcommons.library.umaine.edu/aes_techbulletin/1180/thumbnail.jp

Clinical Outcomes and Cadaveric Biomechanical Analysis of Endoscopic Percutaneous Achilles Tendon Rupture Repair With Absorbable Suture

Background: Percutaneous repair of acute Achilles tendon rupture (ATR) continues to gain in popularity. The primary aim of the study was to review the outcomes of a patient cohort undergoing a novel technique of endoscopic percutaneous Achilles tendon repair with absorbable suture. A secondary purpose of this study was to evaluate the basic biomechanical properties of the technique. Methods: A cohort of 30 patients who underwent percutaneous ATR repair was retrospectively analyzed with Achilles Tendon Rupture Scores (ATRS), complications, and additional outcome measures. For a biomechanical analysis portion of the study, 12 cadaveric specimens were paired and randomized to either novel percutaneous repair or open Kessler repair with absorbable suture. These specimens were subjected to 2 phases of cyclical testing (100 cycles 10-43 N followed by 200 cycles 10-86 N) and ultimate strength testing. Results: In the clinical portion of the study we report excellent patient reported outcomes (mean ATRS 94.1), high level of return to sport, and high patient satisfaction. One partial re-rupture was reported but with no major wound or neurologic complications. In the biomechanical portion of the study we found no significant difference in tendon gapping between percutaneous and open repairs in phase 1 of testing. In phase 2, increased gapping occurred between percutaneous (17.8 mm [range 10.7-24.1, SD 6.4]) and open repairs (10.8 mm [range 7.6-14.9, SD 2.7, P = .037]). The ultimate load at failure was not statistically different between the 2 repairs. Conclusions: A percutaneous ATR repair technique using endoscopic assistance and absorbable suture demonstrated low complications and good outcomes in a cohort of patients, with high satisfaction, and excellent functional outcomes including high rates of return to sport. Cadaveric biomechanical testing demonstrated excellent survival during testing and minimal increase in gapping compared with open repair technique, representing sufficient strength to withstand forces seen in early rehabilitation. A percutaneous Achilles tendon repair technique with absorbable suture may minimize risks associated with operative repair while still maintaining the benefit of operative repair. Level of Evidence: Level IV, retrospective case series

TB30: A Critical Evaluation of Results from Spectographic Analysis of Plan Tissue

For this study, samples of wheat, corn, timothy, orchardgrass, alfalfa, Bermuda grass, and tomato were analyzed for eleven elements. A statistical study of these data was undertaken to determine the precision of the spectrographic analyses and to determine the precision that could be expected from analyses by this method.https://digitalcommons.library.umaine.edu/aes_techbulletin/1169/thumbnail.jp

Sphingosine-1-phosphate promotes the differentiation of adipose-derived stem cells into endothelial nitric oxide synthase (eNOS) expressing endothelial-like cells.

BACKGROUND: Adipose tissue provides a readily available source of autologous stem cells. Adipose-derived stem cells (ASCs) have been proposed as a source for endothelial cell substitutes for lining the luminal surface of tissue engineered bypass grafts. Endothelial nitric oxide synthase (eNOS) is a key protein in endothelial cell function. Currently, endothelial differentiation from ASCs is limited by poor eNOS expression. The goal of this study was to investigate the role of three molecules, sphingosine-1-phosphate (S1P), bradykinin, and prostaglandin-E1 (PGE1) in ASC endothelial differentiation. Endothelial differentiation markers (CD31, vWF and eNOS) were used to evaluate the level of ASCs differentiation capability. RESULTS: ASCs demonstrated differentiation capability toward to adipose, osteocyte and endothelial like cell phenotypes. Bradykinin, S1P and PGE were used to promote differentiation of ASCs to an endothelial phenotype. Real-time PCR showed that all three molecules induced significantly greater expression of endothelial differentiation markers CD31, vWF and eNOS than untreated cells. Among the three molecules, S1P showed the highest up-regulation on endothelial differentiation markers. Immunostaining confirmed presence of more eNOS in cells treated with S1P than the other groups. Cell growth measurements by MTT assay, cell counting and EdU DNA incorporation suggest that S1P promotes cell growth during ASCs endothelial differentiation. The S1P1 receptor was expressed in ASC-differentiated endothelial cells and S1P induced up-regulation of PI3K. CONCLUSIONS: S1P up-regulates endothelial cell markers including eNOS in ASCs differentiated to endothelial like cells. This up-regulation appears to be mediated by the up-regulation of PI3K via S1P1 receptor. ASCs treated with S1P offer promising use as endothelial cell substitutes for tissue engineered vascular grafts and vascular networks

Value of black blood T2* cardiovascular magnetic resonance

Purpose To assess whether black blood T2* cardiovascular magnetic resonance is superior to conventional white blood imaging of cardiac iron in patients with thalassaemia major (TM). Materials and methods We performed both conventional white blood and black blood T2* CMR sequences in 100 TM patients to determine intra and inter-observer variability and presence of artefacts. In 23 patients, 2 separate studies of both techniques were performed to assess interstudy reproducibility. Results Cardiac T2* values ranged from 4.5 to 43.8 ms. The mean T2* values were not different between black blood and white blood acquisitions (20.5 vs 21.6 ms, p = 0.26). Compared with the conventional white blood diastolic acquisition, the coefficient of variance of the black blood CMR technique was superior for intra-observer reproducibility (1.47% vs 4.23%, p < 0.001), inter-observer reproducibility (2.54% vs 4.50%, p < 0.001) and inter-study reproducibility (4.07% vs 8.42%, p = 0.001). Assessment of artefacts showed a superior score for black blood vs white blood scans (4.57 vs 4.25; p < 0.001). Conclusions Black blood T2* CMR has superior reproducibility and reduced imaging artefacts for the assessment of cardiac iron, in comparison with the conventional white blood technique, which make it the preferred technique for clinical practice

Dinosaur tracks from the Kilmaluag Formation (Bathonian, Middle Jurassic) of Score Bay, Isle of Skye, Scotland, UK

Tracks of a juvenile theropod dinosaur with footprint lengths of between 2 and 9 cm as well as adults of the same ichnospecies with footprints of about 15–25 cm in length were found in the Bathonian (Middle Jurassic) Kilmaluag Formation of Score Bay, northwestern Trotternish Peninsula, Isle of Skye, Scotland, UK. Two footprint sizes occur together on the same bedding plane in the central portion of Score Bay, both in situ and on loose blocks. Another horizon containing footprints above this was also identified. The footprints from the lowest horizon were produced in a desiccated silty mud that was covered with sand. A close association of both adults and juveniles with similar travel direction indicated by the footprints may suggest post-hatching care in theropod dinosaurs. Other footprints, produced on a rippled sandy substrate, have been found on the slightly higher bedding plane at this locality. Loose blocks found 130 m to the northeast in the central part of Score Bay have not been correlated with any in situ sediments, but were preserved in a similar manner to those from the higher bedding plane. These tracks represent the youngest dinosaur remains yet found in Scotland

ALMA Fragmented Source Catalog in Orion (FraSCO). I. Outflow interaction within an embedded cluster in OMC-2/FIR 3, FIR 4, and FIR 5

Funding: This work was supported by NAOJ ALMA Scientific Research grant No. 2022-22B. The present study was supported by JSPS KAKENHI grants (JP17H06360, JP17K05387, JP17KK0096, JP21H00046, JP21K03617: MNM, 20K04034: SI). L.A.Z. acknowledges financial support from CONACyT-280775 and UNAM-PAPIIT IN110618 grants, México. This project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 851435).We present a high-angular resolution (∼1″) and wide-field ( 2.′9×1.′9 ) image of the 1.3 mm continuum, CO(J = 2–1) and SiO(J = 5–4) line emissions toward an embedded protocluster, FIR 3, FIR 4, and FIR 5, in the Orion Molecular Cloud 2 obtained from the Atacama Large Millimeter/submillimeter Array. We identify 51 continuum sources, 36 of which are newly identified in this study. Their dust masses, projected sizes, and H2 gas number densities are estimated to be 3.8 × 10−5–1.1 × 10−2 M⊙, 290–2000 au, and 6.4 × 106–3.3 × 108 cm−3, respectively. The results of a Jeans analysis show that ∼80% of the protostellar sources and ∼15% of the prestellar sources are gravitationally bound. We identify 12 molecular outflows traced in the CO(J = 2–1) emission, six of which are newly detected. We spatially resolve shocked gas structures traced by the SiO(J = 5–4) emission in this region for the first time. We identify shocked gas originating from outflows and other shocked regions. These results provide direct evidence of an interaction between dust condensation, FIR 4, and an energetic outflow driven by HOPS-370 located within FIR 3. A comparison of the outflow dynamical timescales, fragmentation timescales, and protostellar ages shows that the previously proposed triggered star formation scenario in FIR 4 is not strongly supported. We also discuss the spatial distribution of filaments identified in our continuum image by comparing it with a previously identified hub-fiber system in the N2H+ line.Publisher PDFPeer reviewe

ALMA Fragmented Source Catalogue in Orion (FraSCO) I. Outflow interaction within an embedded cluster in OMC-2/FIR3, FIR4, and FIR5

We present a high angular resolution ($\sim1"$) and wide-field ($2'.9 \times 1'.9$) image of the 1.3-mm continuum, CO($J$ = 2--1) line, and SiO($J$ = 5--4) line emissions toward an embedded protocluster, FIR3, FIR4, and FIR5, in the Orion Molecular Cloud 2 obtained from the Atacama Large Millimeter/submillimeter Array (ALMA). We identify 51 continuum sources, 36 of which are newly identified in this study. Their dust masses, projected sizes, and $\mathrm{H_2}$ gas number densities are estimated to be $3.8 \times 10^{-5}$--$1.1 \times 10^{-2} \mathrm{M_{\odot}}$, 290--2000 au, and $6.4 \times 10^{6}$--$3.3 \times 10^{8}\,\mathrm{cm^{-3}}$, respectively. The results of a Jeans analysis show that $\sim80\,\%$ of the protostellar sources and $\sim15\,\%$ of the prestellar sources are gravitationally bound. We identify 12 molecular outflows traced in the CO($J$ = 2--1) emission, six of which are newly detected. We spatially resolve shocked gas structures traced by the SiO($J$ = 5--4) emission in this region for the first time. We identify shocked gas originating from outflows and other shocked regions. These results provide direct evidence of an interaction between a dust condensation, FIR4, and an energetic outflow driven by HOPS-370 located within FIR3. A comparison of the outflow dynamical timescales, fragmentation timescales, and protostellar ages shows that the previously proposed triggered star-formation scenario in FIR4 is not strongly supported. We also discuss the spatial distribution of filaments identified in our continuum image by comparing it with a previously identified hub-fiber system in the $\mathrm{N_2H^+}$ line.Comment: 45 pages, 24 figures, 2 figure sets, 2 animations, Accepted for publication in Ap

ALMA Fragmented Source Catalog in Orion (FraSCO). I. Outflow Interaction within an Embedded Cluster in OMC-2/FIR 3, FIR 4, and FIR 5

We present a high-angular resolution (∼1'') and wide-field () image of the 1.3 mm continuum, CO(J = 2–1) and SiO(J = 5–4) line emissions toward an embedded protocluster, FIR 3, FIR 4, and FIR 5, in the Orion Molecular Cloud 2 obtained from the Atacama Large Millimeter/submillimeter Array. We identify 51 continuum sources, 36 of which are newly identified in this study. Their dust masses, projected sizes, and H2 gas number densities are estimated to be 3.8 × 10−5–1.1 × 10−2 M⊙, 290–2000 au, and 6.4 × 106–3.3 × 108 cm−3, respectively. The results of a Jeans analysis show that ∼80% of the protostellar sources and ∼15% of the prestellar sources are gravitationally bound. We identify 12 molecular outflows traced in the CO(J = 2–1) emission, six of which are newly detected. We spatially resolve shocked gas structures traced by the SiO(J = 5–4) emission in this region for the first time. We identify shocked gas originating from outflows and other shocked regions. These results provide direct evidence of an interaction between dust condensation, FIR 4, and an energetic outflow driven by HOPS-370 located within FIR 3. A comparison of the outflow dynamical timescales, fragmentation timescales, and protostellar ages shows that the previously proposed triggered star formation scenario in FIR 4 is not strongly supported. We also discuss the spatial distribution of filaments identified in our continuum image by comparing it with a previously identified hub-fiber system in the N2H+ line