Antibody-mediated neutralization of Ebola virus can occur by two distinct mechanisms

AbstractHuman Ebola virus causes severe hemorrhagic fever disease with high mortality and there is no vaccine or treatment. Antibodies in survivors occur early, are sustained, and can delay infection when transferred into nonhuman primates. Monoclonal antibodies (mAbs) from survivors exhibit potent neutralizing activity in vitro and are protective in rodents. To better understand targets and mechanisms of neutralization, we investigated a panel of mAbs shown previously to react with the envelope glycoprotein (GP). While one non-neutralizing mAb recognized a GP epitope in the nonessential mucin-like domain, the rest were specific for GP1, were neutralizing, and could be further distinguished by reactivity with secreted GP. We show that survivor antibodies, human KZ52 and monkey JP3K11, were specific for conformation-dependent epitopes comprising residues in GP1 and GP2 and that neutralization occurred by two distinct mechanisms; KZ52 inhibited cathepsin cleavage of GP whereas JP3K11 recognized the cleaved, fusion-active form of GP

Immune Protection of Nonhuman Primates against Ebola Virus with Single Low-Dose Adenovirus Vectors Encoding Modified GPs

BACKGROUND: Ebola virus causes a hemorrhagic fever syndrome that is associated with high mortality in humans. In the absence of effective therapies for Ebola virus infection, the development of a vaccine becomes an important strategy to contain outbreaks. Immunization with DNA and/or replication-defective adenoviral vectors (rAd) encoding the Ebola glycoprotein (GP) and nucleoprotein (NP) has been previously shown to confer specific protective immunity in nonhuman primates. GP can exert cytopathic effects on transfected cells in vitro, and multiple GP forms have been identified in nature, raising the question of which would be optimal for a human vaccine. METHODS AND FINDINGS: To address this question, we have explored the efficacy of mutant GPs from multiple Ebola virus strains with reduced in vitro cytopathicity and analyzed their protective effects in the primate challenge model, with or without NP. Deletion of the GP transmembrane domain eliminated in vitro cytopathicity but reduced its protective efficacy by at least one order of magnitude. In contrast, a point mutation was identified that abolished this cytopathicity but retained immunogenicity and conferred immune protection in the absence of NP. The minimal effective rAd dose was established at 10(10) particles, two logs lower than that used previously. CONCLUSIONS: Expression of specific GPs alone vectored by rAd are sufficient to confer protection against lethal challenge in a relevant nonhuman primate model. Elimination of NP from the vaccine and dose reductions to 10(10) rAd particles do not diminish protection and simplify the vaccine, providing the basis for selection of a human vaccine candidate

Elimination of GP Cytopathic Effects and Expression of Transmembrane-Deleted Protein

<div><p>(A) Expression of GP(ΔTM) in HEK293 cells. Ebola GP proteins from supernatants and cell lysates were visualized by SDS-PAGE and Western blot using a polyclonal antibody against Ebola GP.</p> <p>(B) Elimination of cell rounding by GPΔTM. HEK293 cells were transfected with a plasmid that encoded vector control, Ebola GP, or Ebola GPΔTM. Cell monolayers were visualized under phase contrast using a Nikon 40× objective and photographed at 24 h posttransfection.</p></div

Determination of Lowest Vaccine Dose for Immune Protection against Ebola Virus Challenge by Adenoviral Vector GP/NP Vaccine

<div><p>(A) Kaplan-Meier survival curve of macaques: Immunization was with rAd vectors expressing GP(Z) and NP(Z) (10⁹–10¹¹, three animals per group; control and 10¹², one animal each), and challenge was performed with the Kikwit strain of Zaire ebolavirus as in <a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030177#pmed-0030177-g002" target="_blank">Figure 2</a>A. </p> <p>(B) Immune responses in immunized animals. Intracellular flow cytometry was performed to quantify TNF-α production from Ebola-specific CD4⁺ (left graph) or CD8⁺ (right graph) lymphocytes, respectively, from animals immunized as indicated. Immune responses were measured at 3 wk postimmunization. Circles, diamonds, and squares indicate responses for individual animals. Horizontal line indicates the average of individual responses in the immunization group. Results represent the percent cytokine positive in the gated lymphocyte group and background stimulation (DMSO alone) has been subtracted from each sample ( <i>p</i>-values obtained using unpaired Student's <i>t</i>-test; n.s., not significant). </p> <p>(C) Antibody responses in immunized animals. Anti-GP ELISA titers (left graph) and serum neutralizing antibody responses (right graph) were measured as described in <a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030177#s2" target="_blank">Methods</a>. </p></div

Elimination of GP Cytopathic Effects with Single Point Mutation

<div><p>(A) Expression of point mutants in HEK293 cells. Ebola GP proteins from supernatants and cell lysates were visualized by SDS-PAGE and Western blot using a polyclonal antibody against Ebola GP.</p> <p>(B) Reactivity of point mutants with a conformation-dependent antibody. HEK293 cells were transfected with a control plasmid (red line) or plasmids expressing wild-type (GP[Z], blue line), or mutant (E71D[Z], green line) proteins. 18 h posttransfection, cells were harvested and stained with a GP-specific antibody, and cell surface GP expression was analyzed by flow cytometry.</p> <p>(C) Elimination of cell rounding by amino acid substitution at position 71. HEK293 cells were transfected with a plasmid that encoded vector control, wild-type Ebola glycoprotein from Zaire (GP[Z]) or Sudan/Gulu (GP[S/G]), or their respective point mutations (E71D[Z] and E71D[S/G]). Cell monolayers were visualized under phase contrast using a Nikon 40× objective and photographed at 24 h posttransfection.</p></div

Comparative Efficacy of GP and GPΔTM for Protection against Ebola Virus Challenge

<div><p>(A) Kaplan-Meier survival curve of cynomolgus macaques, immunized as indicated, and challenged with 1,000 pfu of Zaire ebolavirus (strain Kikwit) 1 mo post immunization. The <i>x</i>-axis indicates weeks postchallenge. Different immunization groups had <i>n</i> = 3, except for the GP(Z) + NP (10¹²) group ( <i>n</i> = 4), and control ( <i>n</i> = 1). </p> <p>(B) Immune responses in immunized animals. Left and middle graphs: Intracellular flow cytometry was performed to quantify TNF-α production from Ebola-specific CD4⁺ or CD8⁺ lymphocytes, respectively, from animals immunized as indicated (the 10¹² group contains only three CD8 points due to technical error in one sample). Immune responses were measured at 3 wk postimmunization. Circles, diamonds, squares, and triangles indicate responses for individual animals. Horizontal line indicates the average of individual responses in the immunization group. Results represent the percent cytokine-positive in the gated lymphocyte group, and background stimulation (DMSO alone) has been subtracted from each sample. ELISA titers of Ebola GP-specific antibodies in sera of vaccinated animals collected at week 3 postimmunization (right graph). ELISA results represent endpoint dilution titers determined by optical density as described in <a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030177#s2" target="_blank">Methods</a>. </p></div

Comparative Efficacy of Wild-Type and Point Mutant Glycoprotein Vaccines against Lethal Ebola Virus Challenge

<div><p>(A) Kaplan-Meier survival curve of macaques: Immunization and challenge were performed with the Kikwit strain ebolavirus as in <a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030177#pmed-0030177-g002" target="_blank">Figure 2</a>A. </p> <p>(B) Immune responses in immunized animals. Left and middle graphs: Intracellular flow cytometry was performed to quantify TNF-α production from Ebola-specific CD4⁺ or CD8⁺ lymphocytes, respectively, from animals immunized as indicated. Immune responses were measured at 3 wk postimmunization. Circles, diamonds, and squares indicate responses for individual animals. Horizontal line indicates average of individual responses in the immunization group. Results represent the percent cytokine positive in the gated lymphocyte group, and background stimulation (DMSO alone) has been subtracted from each sample. ELISA titers of Ebola GP-specific antibodies in sera of vaccinated animals collected at week 3 postimmunization (right graph). ELISA results represent endpoint dilution titers determined by optical density as described in <a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030177#s2" target="_blank">Methods</a>. </p></div