83 research outputs found
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High Density Genetic Maps of Seashore Paspalum Using Genotyping-By-Sequencing and Their Relationship to The Sorghum Bicolor Genome.
As a step towards trait mapping in the halophyte seashore paspalum (Paspalum vaginatum Sw.), we developed an F1 mapping population from a cross between two genetically diverse and heterozygous accessions, 509022 and HI33. Progeny were genotyped using a genotyping-by-sequencing (GBS) approach and sequence reads were analyzed for single nucleotide polymorphisms (SNPs) using the UGbS-Flex pipeline. More markers were identified that segregated in the maternal parent (HA maps) compared to the paternal parent (AH maps), suggesting that 509022 had overall higher levels of heterozygosity than HI33. We also generated maps that consisted of markers that were heterozygous in both parents (HH maps). The AH, HA and HH maps each comprised more than 1000 markers. Markers formed 10 linkage groups, corresponding to the ten seashore paspalum chromosomes. Comparative analyses showed that each seashore paspalum chromosome was syntenic to and highly colinear with a single sorghum chromosome. Four inversions were identified, two of which were sorghum-specific while the other two were likely specific to seashore paspalum. These high-density maps are the first available genetic maps for seashore paspalum. The maps will provide a valuable tool for plant breeders and others in the Paspalum community to identify traits of interest, including salt tolerance
Carbon dynamics of a warm season turfgrass using the eddy-covariance technique
Despite their ubiquitous presence in the urban landscape throughout the United States, scant attention has been given to evaluate the magnitude of net carbon balance from turfgrasses. Warm season turfgrasses, in particular, have largely been understudied for their carbon sequestration potential. With questions being frequently raised on the environment friendliness of warm season turfgrasses, detailed and robust studies focusing on the carbon behavior of such systems are warranted. This study delves into the carbon balance of ‘Tifway’ bermudagrass, the extensively used warm-season turfgrass in Georgia and other subtropical and warm temperate areas. Using the eddy-covariance method, the amount of CO2 captured by a highly managed turfgrass system was measured by deploying two eddy-covariance systems for the study period of 31 months. The results show that ‘Tifway’ bermudagrass is a net sink of carbon, sequestering it at the rate of 4.51–5.15 Mg C ha−1 yr−1. The turf canopy as well as management activities carried out in the farm appear to have a powerful influence on the carbon behavior of the turf. Seasonal and monthly fluxes suggest that turf is an efficient assimilator of carbon during its active growth period of summer and fall months. The results show that the turf sequestered higher amounts of carbon than many agricultural crop systems, supporting the assertion that it is an efficient assimilator of atmospheric carbon. © 201
Continuous-variable optical quantum state tomography
This review covers latest developments in continuous-variable quantum-state
tomography of optical fields and photons, placing a special accent on its
practical aspects and applications in quantum information technology. Optical
homodyne tomography is reviewed as a method of reconstructing the state of
light in a given optical mode. A range of relevant practical topics are
discussed, such as state-reconstruction algorithms (with emphasis on the
maximum-likelihood technique), the technology of time-domain homodyne
detection, mode matching issues, and engineering of complex quantum states of
light. The paper also surveys quantum-state tomography for the transverse
spatial state (spatial mode) of the field in the special case of fields
containing precisely one photon.Comment: Finally, a revision! Comments to lvov(at)ucalgary.ca and
raymer(at)uoregon.edu are welcom
Radon transform and pattern functions in quantum tomography
The two-dimensional Radon transform of the Wigner quasiprobability is
introduced in canonical form and the functions playing a role in its inversion
are discussed. The transformation properties of this Radon transform with
respect to displacement and squeezing of states are studied and it is shown
that the last is equivalent to a symplectic transformation of the variables of
the Radon transform with the contragredient matrix to the transformation of the
variables in the Wigner quasiprobability. The reconstruction of the density
operator from the Radon transform and the direct reconstruction of its
Fock-state matrix elements and of its normally ordered moments are discussed.
It is found that for finite-order moments the integration over the angle can be
reduced to a finite sum over a discrete set of angles. The reconstruction of
the Fock-state matrix elements from the normally ordered moments leads to a new
representation of the pattern functions by convergent series over even or odd
Hermite polynomials which is appropriate for practical calculations. The
structure of the pattern functions as first derivatives of the products of
normalizable and nonnormalizable eigenfunctions to the number operator is
considered from the point of view of this new representation.Comment: To appear on Journal of Modern Optics.Submitted t
Genetic load and transgenic mitigating genes in transgenic \u3ci\u3eBrassica rapa\u3c/i\u3e (field mustard) × \u3ci\u3eBrassica napus\u3c/i\u3e (oilseed rape) hybrid populations
Abstract Background
One theoretical explanation for the relatively poor performance of Brassica rapa (weed) × Brassica napus (crop) transgenic hybrids suggests that hybridization imparts a negative genetic load. Consequently, in hybrids genetic load could overshadow any benefits of fitness enhancing transgenes and become the limiting factor in transgenic hybrid persistence. Two types of genetic load were analyzed in this study: random/linkage-derived genetic load, and directly incorporated genetic load using a transgenic mitigation (TM) strategy. In order to measure the effects of random genetic load, hybrid productivity (seed yield and biomass) was correlated with crop- and weed-specific AFLP genomic markers. This portion of the study was designed to answer whether or not weed × transgenic crop hybrids possessing more crop genes were less competitive than hybrids containing fewer crop genes. The effects of directly incorporated genetic load (TM) were analyzed through transgene persistence data. TM strategies are proposed to decrease transgene persistence if gene flow and subsequent transgene introgression to a wild host were to occur. Results
In the absence of interspecific competition, transgenic weed × crop hybrids benefited from having more crop-specific alleles. There was a positive correlation between performance and number of B. napus crop-specific AFLP markers [seed yield vs. marker number (r = 0.54, P = 0.0003) and vegetative dry biomass vs. marker number (r = 0.44, P = 0.005)]. However under interspecific competition with wheat or more weed-like conditions (i.e. representing a situation where hybrid plants emerge as volunteer weeds in subsequent cropping systems), there was a positive correlation between the number of B. rapa weed-specific AFLP markers and seed yield (r = 0.70, P = 0.0001), although no such correlation was detected for vegetative biomass. When genetic load was directly incorporated into the hybrid genome, by inserting a fitness-mitigating dwarfing gene that that is beneficial for crops but deleterious for weeds (a transgene mitigation measure), there was a dramatic decrease in the number of transgenic hybrid progeny persisting in the population. Conclusion
The effects of genetic load of crop and in some situations, weed alleles might be beneficial under certain environmental conditions. However, when genetic load was directly incorporated into transgenic events, e.g., using a TM construct, the number of transgenic hybrids and persistence in weedy genomic backgrounds was significantly decreased
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Acetyl-CoA Carboxylase Herbicide Tolerance in Bermudagrass
Contamination of newly planted bermudagrass (Cynodon spp.) varieties by undesirable off-type bermudagrass genotypes is an
ever increasing concern for turf managers because selective control options are limited. In 2009, a sethoxydim {2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one} tolerant bermudagrass genotype (93-175) was identified during
herbicide screening at the University of Georgia in Tifton. The objective of this research was to assess the tolerance of 93-175 to three Acetyl-CoA carboxylase (ACCase) herbicides in comparison to the susceptible genotypes Tifway and common bermudagrass.
Greenhouse and field trials were performed between August 2011 and April 2013. Factors in the field experiment included ACCase
herbicides, application rates, bermudagrass genotypes, and locations. Turfgrass injury ratings taken 42 days after treatment (DAT)
and during greenup the following spring supported initial preliminary findings. At the 1x rate of sethoxydim (280 g a.i. ha⁻¹),
93-175 displayed 50 to 87% less injury in comparison to the susceptible genotypes. In the spring of 2013, 93-175 plots treated with
a 1x rate of sethoxydim reached 100% recovery during the same time period as non-treated controls, while common and Tifway
had only recovered to 48 and 60%, respectively. The tolerance mechanism of 93-175 to sethoxydim did not confer an appreciable
reduction of clethodim {(E,E)-(6)-2-[1-[[(3-chloro-2-propenyl)oxy]imino]propyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-
1-one} or fluazifop {(6)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy]propanoic acid} herbicide treatment effects. 93-175 will
continue to be studied to determine transferability of herbicide tolerance to progeny and the mechanism of the observed tolerance
Self-homodyne tomography of a twin-beam state
A self-homodyne detection scheme is proposed to perform two-mode tomography
on a twin-beam state at the output of a nondegenerate optical parametric
amplifier. This scheme has been devised to improve the matching between the
local oscillator and the signal modes, which is the main limitation to the
overall quantum efficiency in conventional homodyning. The feasibility of the
measurement is analyzed on the basis of Monte-Carlo simulations, studying the
effect of non-unit quantum efficiency on detection of the correlation and the
total photon-number oscillations of the twin-beam state.Comment: 13 pages (two-column ReVTeX) including 21 postscript figures; to
appear on Phys. Rev.
Phase-space formulation of quantum mechanics and quantum state reconstruction for physical systems with Lie-group symmetries
We present a detailed discussion of a general theory of phase-space
distributions, introduced recently by the authors [J. Phys. A {\bf 31}, L9
(1998)]. This theory provides a unified phase-space formulation of quantum
mechanics for physical systems possessing Lie-group symmetries. The concept of
generalized coherent states and the method of harmonic analysis are used to
construct explicitly a family of phase-space functions which are postulated to
satisfy the Stratonovich-Weyl correspondence with a generalized traciality
condition. The symbol calculus for the phase-space functions is given by means
of the generalized twisted product. The phase-space formalism is used to study
the problem of the reconstruction of quantum states. In particular, we consider
the reconstruction method based on measurements of displaced projectors, which
comprises a number of recently proposed quantum-optical schemes and is also
related to the standard methods of signal processing. A general group-theoretic
description of this method is developed using the technique of harmonic
expansions on the phase space.Comment: REVTeX, 18 pages, no figure
Genetic load and transgenic mitigating genes in transgenic Brassica rapa (field mustard) × Brassica napus (oilseed rape) hybrid populations
<p>Abstract</p> <p>Background</p> <p>One theoretical explanation for the relatively poor performance of <it>Brassica rapa </it>(weed) × <it>Brassica napus </it>(crop) transgenic hybrids suggests that hybridization imparts a negative genetic load. Consequently, in hybrids genetic load could overshadow any benefits of fitness enhancing transgenes and become the limiting factor in transgenic hybrid persistence. Two types of genetic load were analyzed in this study: random/linkage-derived genetic load, and directly incorporated genetic load using a transgenic mitigation (TM) strategy. In order to measure the effects of random genetic load, hybrid productivity (seed yield and biomass) was correlated with crop- and weed-specific AFLP genomic markers. This portion of the study was designed to answer whether or not weed × transgenic crop hybrids possessing more crop genes were less competitive than hybrids containing fewer crop genes. The effects of directly incorporated genetic load (TM) were analyzed through transgene persistence data. TM strategies are proposed to decrease transgene persistence if gene flow and subsequent transgene introgression to a wild host were to occur.</p> <p>Results</p> <p>In the absence of interspecific competition, transgenic weed × crop hybrids benefited from having more crop-specific alleles. There was a positive correlation between performance and number of <it>B. napus </it>crop-specific AFLP markers [seed yield vs. marker number (r = 0.54, P = 0.0003) and vegetative dry biomass vs. marker number (r = 0.44, P = 0.005)]. However under interspecific competition with wheat or more weed-like conditions (i.e. representing a situation where hybrid plants emerge as volunteer weeds in subsequent cropping systems), there was a positive correlation between the number of <it>B. rapa </it>weed-specific AFLP markers and seed yield (r = 0.70, P = 0.0001), although no such correlation was detected for vegetative biomass. When genetic load was directly incorporated into the hybrid genome, by inserting a fitness-mitigating dwarfing gene that that is beneficial for crops but deleterious for weeds (a transgene mitigation measure), there was a dramatic decrease in the number of transgenic hybrid progeny persisting in the population.</p> <p>Conclusion</p> <p>The effects of genetic load of crop and in some situations, weed alleles might be beneficial under certain environmental conditions. However, when genetic load was directly incorporated into transgenic events, e.g., using a TM construct, the number of transgenic hybrids and persistence in weedy genomic backgrounds was significantly decreased.</p
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