Discovery and characterization of novel <i>Aspergillus fumigatus</i> mycoviruses - Fig 1

<p>(A) Schematic representation of the genomic organization of the two segments of the novel Aspergillus fumigatus partitivirus-2 (AfuPV2). (B) Alignment of the 5’-UTR regions of related partitiviruses. Conserved nucleotides found in the genomic segments of Aspergillus fumigatus partitiviruses-1 and -2, Botryosphaeria dothidea partivirus-1, and Alternaria alternata partitivirus-1 are indicated.</p

Schematic representation of the genomic organization of the novel narnaviridae Aspergillus fumigatus narnavirus-1 (AfuNV1), Aspergillus fumigatus narnavirus-2 (AfuNV2), and Aspergillus fumigatus mitovirus-1 (AfuMV).

<p>Schematic representation of the genomic organization of the novel narnaviridae Aspergillus fumigatus narnavirus-1 (AfuNV1), Aspergillus fumigatus narnavirus-2 (AfuNV2), and Aspergillus fumigatus mitovirus-1 (AfuMV).</p

Phylogenetic relationships of the family partitiviridae.

<p>An unrooted phylogenetic tree was calculated from a multiple alignment of the RdRp protein using the maximum likelihood method with 1000 bootstrap replicates. The various subgenera are indicated by colored boxes.</p

Phylogenetic relationships of the family Narnaviridae.

<p>An unrooted phylogenetic tree was calculated from a multiple alignment of the RdRp protein using the maximum likelihood method with 1000 bootstrap replicates. The various subgenera are indicated by colored boxes. The narnavirus and mitovirus genera are indicated.</p

Mycovirus genomic RNA segments found in <i>Aspergillus fumigatus</i> strains.

<p>Mycovirus genomic RNA segments found in <i>Aspergillus fumigatus</i> strains.</p

the dataset for asperigillus fumigatus azole resistance

1st: The MIC and MGR of evolved cultures in evolutionary experiment of seven weeks. 2nd: correction analyse between the MIC and MGR of evolved cultures.3rd: dominance test of azole resistance.4th:stability test of fungicides. 5th:The MIC and MGR of ancestor in different environment

Supplementary Information from Evolution of cross-resistance to medical triazoles in <i>Aspergillus fumigatus</i> through selection pressure of environmental fungicides

Methods, supplementary figures, supplementary table

Growth rate of the 4 sequential isolates expressed as radial growth rate on solid V8 agar (a) and as optical densities in fluent medium (b).

<p>The laboratory strain NCP2109 is included as unrelated comparator. In the radial growth experiment (a) the growth rate is expressed in mean diameter (mm) and Kr (mm/hour) after 24–72 hours of two separate experiments performed in triplicate. In the kinetic evaluation of growth over time in liquid medium (b) growth is expressed as optical densities measured every 10 min.</p

In vivo susceptibility of sequential <i>A. fumigatus</i> isolates from a CGD patient failing azole therapy.

<p>Susceptibility is shown as survival curve (a and d), as fungal CFU kidney burden (b and e) and as <i>Aspergillus</i> DNA load in kidney tissue. The early susceptible isolate (isolate no 2) is shown in fig. a, b and c and the late resistant isolate (isolate no 4) in fig. c, d and e. AND: anidulafungin, POS: posaconazole, AND+POS: combination therapy of anidulafungin and posaconazole, d4: day 4, d8: day 8, * P<0.05 compared to control.</p

In vivo virulence of sequential isolates of <i>A. fumigatus</i> in immunosuppressed mice.

<p>Survival of mice in groups of six challenged with each of the four sequential isolates (1–4) or a control isolate (NCPF2109), respectively, is shown. The survival data was combined per isolate, thus each survival curve displays the mortality of six mice challenged with the indicated isolate, two of which were challenged with a high inoculum (5x10⁴ CFU/ml), two with an intermediate inoculum (10⁴ CFU/ml) and two with a low inoculum (2×10³ CFU/ml).</p