Extra-articular synovial chondromatosis of the ankle: Unusual case with radiologic-pathologic correlation.

Extra-articular synovial chondromatosis is a rare entity in the foot and ankle. We present a case of a 49-year-old female who presented for evaluation of a palpable concern following trauma; which was found to represent synovial chondromatosis. This case demonstrates the multimodality imaging findings, including ultrasound and MRI, with histopathologic correlation

Spot Speed Study of Vehicular Traffic on Major Highways in Makurdi Town

Spot speeds of vehicular traffic along Otukpo, Gboko, Lafia and Iorchia-Ayu highways in Makurdi town where examined. Manual traffic count was carried out to ascertain traffic volume per hour per lane on each lane of the sampled road segments, and spot speeds of vehicles travelling in both directions of the highways where randomly measured using a Brushel speed gun at different spots for a period of 12 hours (6: 00 am – 6:00 pm) daily. Data analysis using statistical techniques revealed that vehicles travelled at average speed of 51 km/h, 53 km/h, 63 km/h and 50 km/h on the Otukpo, Gboko, Lafia, and Iorchia-Ayo highways respectively. Also, the range of travel pace and corresponding percentages of traffic (in brackets) on highways in Makurdi town were; 46-56 km/h (57%), (47-57km/h (50%), 55-65(32%) and 46-56(49%) for the Otukpo, Gboko, Lafia and Iorchia-Ayu roads respectively. Therefore speed limit ranging between 50-55km/h was proposed for highways in Makurdi town to guarantee safe travel. The study also revealed that highways in Makurdi town operated at a design speed below design specifications of 80–100 km/h required by the Nigeria Highway Design Manual except the Lafia road that has design speed of 80km/h. Speed calming devices, high traffic volume, geometrical layout or highway capacity and possibly pavement condition were identified as factors affecting vehicles speeds on highways in Makurdi town. Road rehabilitation for improved capacity and traffic volume to satisfy design speed, and use of speed limit warning signs to guarantee travel safety were recommended. Keywords: Highways in Makurdi town, spot speed, speed limit, design speed, travel pace

D-brane Solitons in Supersymmetric Sigma-Models

Massive D=4 N=2 supersymmetric sigma models typically admit domain wall (Q-kink) solutions and string (Q-lump) solutions, both preserving 1/2 supersymmetry. We exhibit a new static 1/4 supersymmetric `kink-lump' solution in which a string ends on a wall, and show that it has an effective realization as a BIon of the D=4 super DBI-action. It is also shown to have a time-dependent Q-kink-lump generalization which reduces to the Q-lump in a limit corresponding to infinite BI magnetic field. All these 1/4 supersymmetric sigma-model solitons are shown to be realized in M-theory as calibrated, or `Q-calibrated', M5-branes in an M-monopole background.Comment: 16 pages, 3 figures, Late

UV Laser-Induced, Time-Resolved Transcriptome Responses of Saccharomyces cerevisiae

We determined the effect on gene transcription of laser-mediated, long-wavelength UV-irradiation of Saccharomyces cerevisiae by RNAseq analysis at times T15, T30, and T60 min after recovery in growth medium. Laser-irradiated cells were viable, and the transcriptional response was transient, with over 400 genes differentially expressed at T15 or T30, returning to basal level transcription by T60. Identification of transcripts exhibiting enhanced differential expression that were unique to UV laser-irradiation were identified by imposing a stringent significance cut-off (P \u3c 0.05, log2 difference \u3e2) then filtering out genes known as environmental stress response (ESR) genes. Using these rigorous criteria, 56 genes were differentially expressed at T15; at T30 differential expression was observed for 57 genes, some of which persisted from T15. Among the highly up-regulated genes were those supporting amino acid metabolic processes sulfur amino acids, methionine, aspartate, cysteine, serine), sulfur regulation (hydrogen sulfite metabolic processes, sulfate assimilation, sulfate reduction), proteasome components, amino acid transporters, and the iron regulon. At T30, the expression profile shifted to expression of transcripts related to catabolic processes (oxidoreductase activity, peptidase activity). Transcripts common to both T15 and T30 suggested an up-regulation of catabolic events, including UV damage response genes, and protein catabolism via proteasome and peptidase activity. Specific genes encoding tRNAs were among the down-regulated genes adding to the suggestion that control of protein biosynthesis was a major response to long-wave UV laser irradiation. These transcriptional responses highlight the remarkable ability of the yeast cell to respond to a UV-induced environmental insult

Supertubes

It is shown that a IIA superstring carrying D0-brane charge can be `blown-up', in a {\it Minkowski vacuum} background, to a (1/4)-supersymmetric tubular D2-brane, supported against collapse by the angular momentum generated by crossed electric and magnetic Born-Infeld fields. This `supertube' can be viewed as a worldvolume realization of the sigma-model Q-lump.Comment: Revision includes mention of some configurations dual to the supertub

An Infinite Swapping Approach to the Rare-Event Sampling Problem

We describe a new approach to the rare-event Monte Carlo sampling problem. This technique utilizes a symmetrization strategy to create probability distributions that are more highly connected and thus more easily sampled than their original, potentially sparse counterparts. After discussing the formal outline of the approach and devising techniques for its practical implementation, we illustrate the utility of the technique with a series of numerical applications to Lennard-Jones clusters of varying complexity and rare-event character.Comment: 24 pages, 16 figure

Supersymmetric Intersecting Domain Walls in Massive Hyper-Kahler Sigma Models

The general scalar potential of D-dimensional massive sigma-models with eight supersymmetries is found for $D=3,4$. These sigma models typically admit 1/2 supersymmetric domain wall solutions and we find, for a particular hyper-K\"ahler target, exact 1/4 supersymmetric static solutions representing a non-trivial intersection of two domain walls. We also show that the intersecting domain walls can carry Noether charge while preserving 1/4 supersymmetry. We briefly discuss an application to the D1-D5 brane system.Comment: 14 pages, Latex. Various changes including the inclusion of an exact intersecting domain wall solutio

Saliva samples are a viable alternative to blood samples as a source of DNA for high throughput genotyping.

BACKGROUND: The increasing trend for incorporation of biological sample collection within clinical trials requires sample collection procedures which are convenient and acceptable for both patients and clinicians. This study investigated the feasibility of using saliva-extracted DNA in comparison to blood-derived DNA, across two genotyping platforms: Applied Biosystems Taqman™ and Illumina Beadchip™ genome-wide arrays. METHOD: Patients were recruited from the Pharmacogenetics of Breast Cancer Chemotherapy (PGSNPS) study. Paired blood and saliva samples were collected from 79 study participants. The Oragene DNA Self-Collection kit (DNAgenotek®) was used to collect and extract DNA from saliva. DNA from EDTA blood samples (median volume 8 ml) was extracted by Gen-Probe, Livingstone, UK. DNA yields, standard measures of DNA quality, genotype call rates and genotype concordance between paired, duplicated samples were assessed. RESULTS: Total DNA yields were lower from saliva (mean 24 μg, range 0.2-52 μg) than from blood (mean 210 μg, range 58-577 μg) and a 2-fold difference remained after adjusting for the volume of biological material collected. Protein contamination and DNA fragmentation measures were greater in saliva DNA. 78/79 saliva samples yielded sufficient DNA for use on Illumina Beadchip arrays and using Taqman assays. Four samples were randomly selected for genotyping in duplicate on the Illumina Beadchip arrays. All samples were genotyped using Taqman assays. DNA quality, as assessed by genotype call rates and genotype concordance between matched pairs of DNA was high (>97%) for each measure in both blood and saliva-derived DNA. CONCLUSION: We conclude that DNA from saliva and blood samples is comparable when genotyping using either Taqman assays or genome-wide chip arrays. Saliva sampling has the potential to increase participant recruitment within clinical trials, as well as reducing the resources and organisation required for multicentre sample collection.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

Fbxl17 is rearranged in breast cancer and loss of its activity leads to increased global O -GlcNAcylation

Funder: Wildy Fellowship Department of PathologyFunder: Addenbrooke's Charitable Trust, Cambridge University Hospitals; doi: http://dx.doi.org/10.13039/501100002927Funder: The Mark FoundationAbstract: In cancer, many genes are mutated by genome rearrangement, but our understanding of the functional consequences of this remains rudimentary. Here we report the F-box protein encoded by FBXL17 is disrupted in the region of the gene that encodes its substrate-binding leucine rich repeat (LRR) domain. Truncating Fbxl17 LRRs impaired its association with the other SCF holoenzyme subunits Skp1, Cul1 and Rbx1, and decreased ubiquitination activity. Loss of the LRRs also differentially affected Fbxl17 binding to its targets. Thus, genomic rearrangements in FBXL17 are likely to disrupt SCFFbxl17-regulated networks in cancer cells. To investigate the functional effect of these rearrangements, we performed a yeast two-hybrid screen to identify Fbxl17-interacting proteins. Among the 37 binding partners Uap1, an enzyme involved in O-GlcNAcylation of proteins was identified most frequently. We demonstrate that Fbxl17 binds to UAP1 directly and inhibits its phosphorylation, which we propose regulates UAP1 activity. Knockdown of Fbxl17 expression elevated O-GlcNAcylation in breast cancer cells, arguing for a functional role for Fbxl17 in this metabolic pathway