Improving the biopharmaceutical attributes of mangiferin using vitamin E-TPGS co-loaded self-assembled phosholipidic nano-mixed micellar systems

The current research work encompasses the development, characterization, and evaluation of self-assembled phospholipidic nano-mixed miceller system (SPNMS) of a poorly soluble BCS Class IV xanthone bioactive, mangiferin (Mgf) functionalized with co-delivery of vitamin E TPGS. Systematic optimization using I-optimal design yielded self-assembled phospholipidic nano-micelles with a particle size of  80% of drug release in 15 min. The cytotoxicity and cellular uptake studies performed using MCF-7 and MDA-MB-231 cell lines demonstrated greater kill and faster cellular uptake. The ex vivo intestinal permeability revealed higher lymphatic uptake, while in situ perfusion and in vivo pharmacokinetic studies indicated nearly 6.6- and 3.0-folds augmentation in permeability and bioavailability of Mgf. In a nutshell, vitamin E functionalized SPNMS of Mgf improved the biopharmaceutical performance of Mgf in rats for enhanced anticancer potency

NATURAL POLYMER VIS A VIS SYNTHETIC POLYMER FOR SOLUBILITY ENHANCEMENT OF SIMVASTATIN

The enhancement of the oral bioavailability is currently one of the greatest challenges in the development of poorly water soluble drugs. The main objective of work to enhance solubility of Simvastatin (SIM) by use of natural polymer, chitosan (CHI) and Hydroxy propyl methyl cellulose E3LV (HPMCE3LV), synthetic polymer to produce cost effective formulation. Physical mixture, co-grinding method, spray drying method are compared .Co-grinding method applied for preparation of drug polymer complex and compared with the solubility and dissolution of marketed preparation

Marginal donor kidney in a marginal recipient: Five year follow-up

The widening gap between demand and supply of organs became apparent as organ shortage became more severe. Organs previously considered unsuitable for transplantation are currently being used. Autosomal dominant polycystic kidney disease (ADPKD) is a hereditary disease characterized by slow progressive cystic changes and deterioration of renal function. We provide our experience with an ADPKD patient who received a kidney from 38-year-old deceased donor ADPKD-affected kidney for renal transplantation

The role of Piezo1 and Piezo2 proteins in tissue engineering: A Comprehensive review

Almost every life form, from the tiniest bacterium to humans, is mechanosensitive, implying it can use mechanical stresses to trigger certain physiological responses in the form of electric signals. Mechanotransduction largely relies on ion channels that respond to mechanical forces, such as the epithelial sodium channels/degenerins, transient receptor potential channel, and the two-pore domain potassium channel. Piezo1 and Piezo2 proteins were discovered to be the biggest non-selective mechanosensitive cation channels in the cell membrane. A substantial amount of research has previously been published on the Piezo channel's function in touch sensation, balance, and cardiovascular regression. However, the mechanistic perspective must be refined to fully understand the role of Piezo proteins in tissue engineering. This review centers on the latest insights into the structure of Piezo channels, activation mechanisms, and its interactions with cytoskeletal components, by emphasizing the physiological activities of Piezo channels in different tissues. The study also places focus on the possibilities of targeting this cation channel family as a tissue regeneration aid