Evening Methane Emission Pulses from a Boreal Wetland Correspond to Convective Mixing in Hollows

Spatial and temporal heterogeneity of methane flux from boreal wetlands makes prediction and up-scaling challenging, both within and among wetland systems. Drivers of methane production and emissions are also highly variable, making empirical model development difficult and leading to uncertainty in methane emissions estimates from wetlands. Previous studies have examined this problem using point-scale (static chamber method) and ecosystem-scale (flux tower methods) measurements, but few studies have investigated whether different processes are observed at these scales. We analyzed methane emissions from a boreal fen, measured by both techniques, using data from the Boreal Ecosystem-Atmosphere Study. We sought to identify driving processes associated with methane emissions at two scales and explain diurnal patterns in emissions measured by the tower. The mean methane emission rates from flux chambers were greater than the daytime, daily mean rates measured by the tower, but the nighttime, daily mean emissions from the tower were often an order of magnitude greater than emissions recorded during the daytime. Thus, daytime measurements from either the tower or chambers would lead to a biased estimate of total methane emissions from the wetland. We found that the timing of nighttime emission events was coincident with the cooling and convective mixing within hollows, which occurred regularly during the growing season. We propose that diurnal thermal stratification in shallow pools traps methane by limiting turbulent transport. This methane stored during daytime heating is later released during evening cooling due to convective turbulent mixing

Triclosan: An Instructive Tale

The Food and Drug Administration (FDA) recently released a final rule to ban triclosan and 18 other antimicrobial chemicals from soaps. We applaud this rule specifically because of the associated risks that triclosan poses to the spread of antibiotic resistance throughout the environment. This persistent chemical constantly stresses bacteria to adapt, and behavior that promotes antibiotic resistance needs to be stopped immediately when the benefits are null

The Impact of Triclosan on the Spread of Antibiotic Resistance in the Environment

Triclosan (TCS) is a commonly used antimicrobial agent that enters wastewater treatment plants (WWTPs) and the environment. An estimated 1.1 × 105 to 4.2 × 105 kg of TCS are discharged from these WWTPs per year in the United States. The abundance of TCS along with its antimicrobial properties have given rise to concern regarding its impact on antibiotic resistance in the environment. The objective of this review is to assess the state of knowledge regarding the impact of TCS on multidrug resistance in environmental settings, including engineered environments such as anaerobic digesters. Pure culture studies are reviewed in this paper to gain insight into the substantially smaller body of research surrounding the impacts of TCS on environmental microbial communities. Pure culture studies, mainly on pathogenic strains of bacteria, demonstrate that TCS is often associated with multidrug resistance. Research is lacking to quantify the current impacts of TCS discharge to the environment, but it is known that resistance to TCS and multidrug resistance can increase in environmental microbial communities exposed to TCS. Research plans are proposed to quantitatively define the conditions under which TCS selects for multidrug resistance in the environment

Altered Antibiotic Tolerance in Anaerobic Digesters Acclimated to Triclosan Or Triclocarban

Bench-scale anaerobic digesters were amended to elevated steady-state concentrations of triclosan (850 mg/kg) and triclocarban (150 mg/kg) using a synthetic feed. After more than 9 solids retention time (SRT) values of acclimatization, biomass from each digester (and a control digester that received no antimicrobials) was used to assess the toxicity of three antibiotics. Methane production rate was measured as a surrogate for activity in microcosms that received doses of antibiotics ranging from no-antibiotic to inhibitory concentrations. Biomass amended with triclocarban was more sensitive to tetracycline compared to the control indicating synergistic inhibitory effects between this antibiotic and triclocarban. In contrast, biomass amended with triclosan was able to tolerate statistically higher levels of ciprofloxacin indicating that triclosan can induce functional resistance to ciprofloxacin in an anaerobic digester community

Autocatalytic Pyrolysis of Wastewater Biosolids for Product Upgrading

The main goals for sustainable water resource recovery include maximizing energy generation, minimizing adverse environmental impacts, and recovering beneficial resources. Wastewater biosolids pyrolysis is a promising technology that could help facilities reach these goals because it produces biochar that is a valuable soil amendment as well as bio-oil and pyrolysis gas (py-gas) that can be used for energy. The raw bio-oil, however, is corrosive; therefore, employing it as fuel is challenging using standard equipment. A novel pyrolysis process using wastewater biosolids-derived biochar (WB-biochar) as a catalyst was investigated to decrease bio-oil and increase py-gas yield for easier energy recovery. WB-biochar catalyst increased the py-gas yield nearly 2-fold, while decreasing bio-oil production. The catalyzed bio-oil also contained fewer constituents based on GC-MS and GC-FID analyses. The energy shifted from bio-oil to py-gas, indicating the potential for easier on-site energy recovery using the relatively clean py-gas. The metals contained in wastewater biosolids played an important role in upgrading pyrolysis products. The Ca and Fe in WB-biochar reduced bio-oil yield and increased py-gas yield. The py-gas energy increase may be especially useful at water resource recovery facilities that already combust anaerobic digester biogas for energy since it may be possible to blend biogas and py-gas for combined use

On the relevance of numerical simulations to booming sand

We have performed a simulation study of 3D cohesionless granular flows down an inclined chute. We find that the oscillations observed in [L.E. Silbert, Phys. Rev. Lett., 94, 098002 (2005)] near the angle of repose are harmonic vibrations of the lowest normal mode. Their frequencies depend on the contact stiffness as well as on the depth of the flow. Could these oscillations account for the phenomena of "booming sand"? We estimate an effective contact stiffness from the Hertz law, but this leads to frequencies several times higher than observed. However, the Hertz law also predicts interpenetrations of a few nanometers, indicating that the oscillations frequencies are governed by the surface stiffness, which can be much lower than the bulk one. This is in agreement with previous studies ascribing the ability to sing to the presence of a soft coating on the grain surface.Comment: accepted for publication in Physical Review E http://pre.aps.org; Physical Review E (2012) to be publishe

Curriculum Innovation: Incorporating the Kern Engineering Entrepreneurial Network (KEEN) Framework into Online Discussions

The purpose of this study was to respond to the following research question: How does the Kern Engineering Entrepreneurial Network (KEEN) framework build interest in technical topic areas, impact student learning outcomes, and develop the entrepreneurial mindset when applied to the engineering classroom? The KEEN framework was developed to combine the entrepreneurial mindset with engineering education to produce a more valuable, strategically prepared engineer, rather than simply an “obedient engineer”. The framework proposes that the entrepreneurial mindset of students is increased by promoting curiosity, encouraging connections, and creating value. The results from this work provide insight into the impact and implications resulting from applying the KEEN framework to the engineering classroom via online discussions

Triclosan Adsorption Using Wastewater Biosolids-derived Biochar

Organic micropollutants are ubiquitous in the environment and stem from municipal wastewater treatment plant discharges. Adsorption can be used as a tertiary treatment to complement the conventional activated sludge process to remove micropollutants prior to discharge. This research evaluated the performance of wastewater biosolids-derived biochar as an adsorbent to remove triclosan from water. Pre-conditioning of the biochar using hydrochloric acid (HCl) was an essential step for triclosan adsorption. Using acid-conditioned biochar, maximum adsorption of 872 μg triclosan per g biochar was achieved with biochar produced at 800 °C. Biochar produced at higher pyrolysis temperatures tended to have higher triclosan sorption capacity using initial triclosan concentrations of 200 μg L−1 levels. However, pyrolysis temperature had less impact on triclosan sorption at lower, environmentally relevant concentrations. Low solution pH (3) enhanced adsorption and high pH (11) inhibited adsorption. Effective triclosan sorption was observed between pH 5 and 9, with little variation, which is positive for practical applications operated at near-neutral solution pH. In wastewater, acid-treated biochar also effectively sorbed triclosan, albeit at a decreased adsorption capacity and removal rate due to competition from other organic constituents. This study indicated that adsorption may occur mainly due to high surface area, hydrophobicity, and potential interaction between biochar and triclosan functional groups including hydrogen bonding and π-stacking. This work demonstrated that acid-conditioned biosolids-derived biochar could be a suitable sorbent to remove triclosan from wastewater as a final polishing treatment step

Effect of Pyrolysis on the Removal of Antibiotic Resistance Genes and Class I Integrons from Municipal Wastewater Biosolids

Wastewater biosolids represent a significant reservoir of antibiotic resistance genes (ARGs). While current biosolids treatment technologies can reduce ARG levels in residual wastewater biosolids, observed removal rates vary substantially. Pyrolysis is an anoxic thermal degradation process that can be used to convert biosolids into energy rich products including py-gas and py-oil, and a beneficial soil amendment, biochar. Batch pyrolysis experiments conducted on municipal biosolids revealed that the 16S rRNA gene, the ARGs erm(B), sul1, tet(L), tet(O), and the integrase gene of class 1 integrons (intI1) were significantly reduced at pyrolysis temperatures ranging from 300–700 °C, as determined by quantitative polymerase chain reaction (qPCR). Pyrolysis of biosolids at 500 °C and higher resulted in approximately 6-log removal of the bacterial 16S rRNA gene. ARGs with the highest observed removals were sul1 and tet(O), which had observed reductions of 4.62 and 4.04-log, respectively. Pyrolysis reaction time had a significant impact on 16S rRNA, ARG and intI1 levels. A pyrolysis residence time of 5 minutes at 500 °C reduced all genes to below detection limits. These results demonstrate that pyrolysis could be implemented as a biosolids polishing treatment technology to substantially decrease the abundance of total bacteria (i.e., 16S rRNA), ARGs and intI1 prior to land application of municipal biosolids

Pyrolysis of Wastewater Biosolids Significantly Reduces Estrogenicity

Most wastewater treatment processes are not specifically designed to remove micropollutants. Many micropollutants are hydrophobic so they remain in the biosolids and are discharged to the environment through land-application of biosolids. Micropollutants encompass a broad range of organic chemicals, including estrogenic compounds (natural and synthetic) that reside in the environment, a.k.a. environmental estrogens. Public concern over land application of biosolids stemming from the occurrence of micropollutants hampers the value of biosolids which are important to wastewater treatment plants as a valuable by-product. This research evaluated pyrolysis, the partial decomposition of organic material in an oxygen-deprived system under high temperatures, as a biosolids treatment process that could remove estrogenic compounds from solids while producing a less hormonally active biochar for soil amendment. The estrogenicity, measured in estradiol equivalents (EEQ) by the yeast estrogen screen (YES) assay, of pyrolyzed biosolids was compared to primary and anaerobically digested biosolids. The estrogenic responses from primary solids and anaerobically digested solids were not statistically significantly different, but pyrolysis of anaerobically digested solids resulted in a significant reduction in EEQ; increasing pyrolysis temperature from 100 °C to 500 °C increased the removal of EEQ with greater than 95% removal occurring at or above 400 °C. This research demonstrates that biosolids treatment with pyrolysis would substantially decrease (removal \u3e 95%) the estrogens associated with this biosolids product. Thus, pyrolysis of biosolids can be used to produce a valuable soil amendment product, biochar, that minimizes discharge of estrogens to the environment