Qualidade de vida no trabalho nivel de estresse dos profissionais das UBS do Distrito Leste do Município de Foz do Iguaçu-PR

Trabalho de conclusão de Residência apresentado ao Programa Multiprofissional em Saúde da Família da Universidade Federal da Integração Latino-Americana, como requisito para obtenção do título de Especialista em Saúde da Família na modalidade de Residência. Orientador: Prof. Dr Thiago Luis de Andrade Barbosa Co-orientador: Prof. Ms. Gilberto Garcia da RochaObjetivo: Avaliar a qualidade de vida no trabalho (QVT) e nível de estresse dos trabalhadores da Atenção Primária à Saúde (APS) do distrito leste de Foz do Iguaçu-PR. Metodologia: Trata-se de estudo transversal no qual participaram 120 profissionais da APS pertencentes a 06 UBS do referido distrito. Os profissionais responderam questionários sociodemográfico, de QVT abreviado (QWLQ-Bref) e Escala do Estresse Percebido (PSS 13). Verificou-se associação da QVT e dos níveis de estresse com variáveis sociodemográficas com uso do teste t de Student, ANOVA, Mann-Whitney e Kruskal-Wallis. Nível de significância adotado foi de 5%. Resultados: A avaliação da QVT dos profissionais foi 62,8±10,0 e associou-se com sexo (p=0,016), idade (p=0,042), presença de dor (p=0,029) e satisfação com trabalho (p=0,002). Em relação aos domínios avaliados da QVT, houve associação com presença de dor e satisfação laboral. O escore total médio de estresse percebido dos participantes foi 24,5±6,0. Notou-se relação com as variáveis percepção quanto à alimentação saudável (p=0,013), presença de dor (p=0,002), dor relacionada ao trabalho (p=0,004) e satisfação com trabalho (p=0,001). Conclusão: Constatou-se satisfatória QVT e níveis médios de estresse dos profissionais o que demanda ações preventivas que melhorem esse panorama na APS do municípioObjective: To evaluate the quality of working life (QWL) and stress level of workers at Primary Health Care (PHC) in the east district of Foz do Iguaçu-PR. Methodology: Cross- sectional study with 120 PHC professionals from 06 basic health units of this district. Professionals answered sociodemographic questionnaire, abbreviated QLW (QWLQ-Bref) and Perceived Stress Scale (PSS 13). We verified the association of QWL and stress levels with sociodemographic variables using Student's t-test, ANOVA, Mann-Whitney and Kruskal-Wallis. Level of significance was 5%. Results: The professional QWL assessment was 62.8 ± 10.0 and was associated with gender (p=0.016), age (p=0.042), presence of pain (p = 0.029) and work satisfaction (p=0.002). In relation to the evaluated domains of QWL, there was an association with presence of pain and job satisfaction. The mean total perceived stress score of the participants was 24.5 ± 6.0. There was a relationship with the variables of perception regarding healthy eating (p=0.013), presence of pain (p=0.002), pain related to work (p=0.004) and satisfaction with work (p=0.001). Conclusion: We observed satisfactory QWL and mean levels of stress of PHC workers that demand improvement of this situationObjetivo: Evaluar la calidad de vida en el trabajo (QVT) y nivel de estrés de los trabajadores de la Atención Primaria a la Salud (APS) del distrito este de Foz do Iguaçu-PR. Metodología: Se trata de un estudio transversal en el que participaron 120 profesionales de la APS pertenecientes a 06 UBS de dicho distrito. Los profesionales respondieron cuestionarios sociodemográficos, de QVT abreviado (QWLQ-Bref) y Escala del estrés percibido (PSS 13). Se verificó asociación de QVT y de los niveles de estrés con variables sociodemográficas con el uso del test t de Student, ANOVA, Mann-Whitney y Kruskal-Wallis. El nivel de significancia adoptado fue del 5%. Resultados: La evaluación de la QVT de los profesionales fue de 62,8 ± 10,0 y se asoció con sexo (p=0,016), edad (p=0,042), presencia de dolor (p=0,029) y satisfacción con el trabajo (p=0,029) 0,002). En relación a los ámbitos evaluados de la QVT, hubo asociación con presencia de dolor y satisfacción laboral. La puntuación total promedio de estrés percibido de los participantes fue de 24,5 ± 6,0. Se observó relación con las variables percepción en cuanto a la alimentación sana (p=0,013), presencia de dolor (p=0,002), dolor relacionado al trabajo (p=0,004) y satisfacción con el trabajo (p=0,001). Conclusión: Se constató satisfactoria QVT y niveles medios de estrés de los profesionales lo que demanda acciones preventivas que mejoren ese panorama en la APS del municipi

Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury

Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine (8-OHdG), 4-hydroxy-2-nonenal (4-HNE) and 3-nitrotyrosin (3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and NeuN. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHdG, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and NeuN in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death