Molecular Genetic Typing of Staphylococcus aureus from Cows, Goats, Sheep, Rabbits and Chickens

End of project reportsS. aureus can also cause a number of infections in animals such as tick-associated pyaemia in lambs, staphylococcosis in rabbits, septicaemia, abscesses and chondronecrosis in chickens and pneumonia and osteomyelitis complex in turkeys. S. aureus is the most frequent cause of bovine mastitis, a disease that is of economic importance worldwide (Beck et al., 1992). Typically staphylococcal mastitis is chronic in nature, with subclinical mastitis being the most common form

Magnetic Fields in Stellar Jets

Although several lines of evidence suggest that jets from young stars are driven magnetically from accretion disks, existing observations of field strengths in the bow shocks of these flows imply that magnetic fields play only a minor role in the dynamics at these locations. To investigate this apparent discrepancy we performed numerical simulations of expanding magnetized jets with stochastically variable input velocities with the AstroBEAR MHD code. Because the magnetic field B is proportional to the density n within compression and rarefaction regions, the magnetic signal speed drops in rarefactions and increases in the compressed areas of velocity-variable flows. In contrast, B ~ n^0.5 for a steady-state conical flow with a toroidal field, so the Alfven speed in that case is constant along the entire jet. The simulations show that the combined effects of shocks, rarefactions, and divergent flow cause magnetic fields to scale with density as an intermediate power 1 > p > 0.5. Because p > 0.5, the Alfven speed in rarefactions decreases on average as the jet propagates away from the star. This behavior is extremely important to the flow dynamics because it means that a typical Alfven velocity in the jet close to the star is significantly larger than it is in the rarefactions ahead of bow shocks at larger distances, the one place where the field is a measurable quantity. We find that the observed values of weak fields at large distances are consistent with strong fields required to drive the observed mass loss close to the star. For a typical stellar jet the crossover point inside which velocity perturbations of 30 - 40 km/s no longer produce shocks is ~ 300 AU from the source

A New Look at T Tauri Star Forbidden Lines: MHD Driven Winds from the Inner Disk

Magnetohydrodynamic (MHD) and photoevaporative winds are thought to play an important role in the evolution and dispersal of planet-forming disks. We report the first high-resolution ($\Delta v\sim$6\kms) analysis of [S II] $\lambda$4068, [O I] $\lambda$5577, and [O I] $\lambda$6300 lines from a sample of 48 T Tauri stars. Following Simon et al. (2016), we decompose them into three kinematic components: a high-velocity component (HVC) associated with jets, and a low-velocity narrow (LVC-NC) and broad (LVC-BC) components. We confirm previous findings that many LVCs are blueshifted by more than 1.5 kms$^{-1}$ thus most likely trace a slow disk wind. We further show that the profiles of individual components are similar in the three lines. We find that most LVC-BC and NC line ratios are explained by thermally excited gas with temperatures between 5,000$-$10,000 K and electron densities $\sim10^{7}-10^{8}$ cm$^{-3}$. The HVC ratios are better reproduced by shock models with a pre-shock H number density of $\sim10^{6}-10^{7}$ cm$^{-3}$. Using these physical properties, we estimate $\dot{M}_{\rm wind}/\dot{M}_{\rm acc}$ for the LVC and $\dot{M}_{\rm jet}/\dot{M}_{\rm acc}$ for the HVC. In agreement with previous work, the mass carried out in jets is modest compared to the accretion rate. With the likely assumption that the NC wind height is larger than the BC, the LVC-BC $\dot{M}_{\rm wind}/\dot{M}_{\rm acc}$ is found to be higher than the LVC-NC. These results suggest that most of the mass loss occurs close to the central star, within a few au, through an MHD driven wind. Depending on the wind height, MHD winds might play a major role in the evolution of the disk mass.Comment: 45 pages, 23 figures, and 7 tables, accepted by Ap

Population and Virulence Factor Analysis of Staphylococcus aureus from Bovine Mastitis.

End of Project ReportsStaphylococcus aureus is a major cause of bovine mastitis and the disease is responsible for substantial economic losses in the dairy industry world-wide. A large number of commonly accepted virulence factors are associated with S. aureus but it is yet to be elucidated which of these are important for infection of the bovine udder. A rational and effective strategy for the control of intramammary infections may need to be directed against clones of S. aureus that commonly cause disease. The objective of this study was to characterise the genetic variance of S. aureus isolate populations from infected udders in Ireland using RAPD-PCR, ribotyping and multilocus enzyme electrophoresis (MLEE). Similar S. aureus isolates collected in the USA were also typed in order to compare strain differences in staphylococcal populations in a different environment. Phenotypic diversity based on a number of presumed virulence factors together with antibiotic sensitivity was examined and correlations between phenotype and genotype were identified, if present. In addition, a pathogenicity island encoding multiple superantigens was completely sequenced and characterised. Knockout mutants of these superantigens were also constructed and in vitro functional analysis performed. Laboratory animal experiments (mice and rabbits) were used to study the relative pathogenicity of individual staphylococcal strains (mice) and also to measure the immunological responses after prolonged exposure to the predominant strains (rabbits)

A Spectroscopic Survey of Subarcsecond Binaries in the Taurus-Auriga Dark Cloud with the Hubble Space Telescope

We report the results of a spectroscopic survey of 20 close T Tauri binaries in the Taurus-Auriga dark cloud where the separations between primaries and their secondaries are less than the typical size of a circumstellar disk around a young star. Analysis of low-resolution and medium-resolution STIS spectra yields the stellar luminosities, reddenings, ages, masses, mass accretion rates, IR excesses, and emission line luminosities for each star in each pair. We examine the ability of IR color excesses, H-alpha equivalent widths, [O I] emission, and veiling to distinguish between weak emission and classical T Tauri stars. Four pairs have one cTTs and one wTTs; the cTTs is the primary in three of these systems. This frequency of mixed pairs among the close T Tauri binaries is similar to the frequency of mixed pairs in wider young binaries. Extinctions within pairs are usually similar; however, the secondary is more heavily reddened than the primary in some systems, where it may be viewed through the primary's disk. Mass accretion rates of primaries and secondaries are strongly correlated, and H-alpha luminosities, IR excesses, and ages also correlate within pairs. Primaries tend to have somewhat larger accretion rates than their secondaries do, and are typically slightly older than their secondaries according to three different sets of modern pre-main-sequence evolutionary tracks. Age differences for XZ Tau and FS Tau, systems embedded in reflection nebulae, are striking; the secondary in each pair is less massive but more luminous than the primary. The stellar masses of the UY Aur and GG Tau binaries measured from their rotating molecular disks are about 30% larger than the masses inferred from the spectra and evolutionary tracks

SpinSpotter: An Automated Algorithm for Identifying Stellar Rotation Periods With Autocorrelation Analysis

Spinspotter is a robust and automated algorithm designed to extract stellar rotation periods from large photometric datasets with minimal supervision. Our approach uses the autocorrelation function (ACF) to identify stellar rotation periods up to one-third the observational baseline of the data. Our algorithm also provides a suite of diagnostics that describe the features in the ACF, which allows the user to fine-tune the tolerance with which to accept a period detection. We apply it to approximately 130,000 main-sequence stars observed by the Transiting Exoplanet Survey Satellite (TESS) at 2-minute cadence during Sectors 1-26, and identify rotation periods for 13,504 stars ranging from 0.4 to 14 days. We demonstrate good agreement between our sample and known values from the literature and note key differences between our population of rotators and those previously identified in the Kepler field, most notably a large population of fast-rotating M dwarfs. Our sample of rotating stars provides a data set with coverage of nearly the entire sky that can be used as a basis for future gyrochronological studies, and, when combined with proper motions and distances from Gaia, to search for regions with high densities of young stars, thus identifying areas of recent star formation and undiscovered moving group members. Our algorithm is publicly available for download and use on GitHub.Comment: 14 pages, 5 figures, Accepted for publication in The Astrophysical Journa