Methane flux from the Central Amazonian Floodplain

A total of 186 methane measurements from the three primary Amazon floodplain environments of open water lakes, flood forests, and floating grass mats were made over the period 18 July through 2 September 1985. These data indicate that emissions were lowest over open water lakes. Flux from flooded forests and grass mats was significantly higher. At least three transport processes contribute to tropospheric emissions: ebullition from sediments, diffusion along the concentration gradient from sediment to overlaying water to air, and transport through the roots and stems of aquatic plants. Measurements indicate that the first two of these processes are most significant. It was estimated that on the average bubbling makes up 49% of the flux from open water, 54% of that from flooded forests, and 64% of that from floating mats. If the measurements were applied to the entire Amazonian floodplain, it is calculated that the region could supply up to 12% of the estimated global natural sources of methane

Methane exchange in a boreal forest estimated by gradient method

Forests are generally considered to be net sinks of atmospheric methane (CH4) because of oxidation by methanotrophic bacteria in well-aerated forests soils. However, emissions from wet forest soils, and sometimes canopy fluxes, are often neglected when quantifying the CH4 budget of a forest. We used a modified Bowen ratio method and combined eddy covariance and gradient methods to estimate net CH4 exchange at a boreal forest site in central Sweden. Results indicate that the site is a net source of CH4. This is in contrast to soil, branch and leaf chamber measurements of uptake of CH4. Wetter soils within the footprint of the canopy are thought to be responsible for the discrepancy. We found no evidence for canopy emissions per se. However, the diel pattern of the CH4 exchange with minimum emissions at daytime correlated well with gross primary production, which supports an uptake in the canopy. More distant source areas could also contribute to the diel pattern; their contribution might be greater at night during stable boundary layer conditions

Multiple novel prostate cancer susceptibility signals identified by fine-mapping of known risk loci among Europeans

Genome-wide association studies (GWAS) have identified numerous common prostate cancer (PrCa) susceptibility loci. We have fine-mapped 64 GWAS regions known at the conclusion of the iCOGS study using large-scale genotyping and imputation in 25 723 PrCa cases and 26 274 controls of European ancestry. We detected evidence for multiple independent signals at 16 regions, 12 of which contained additional newly identified significant associations. A single signal comprising a spectrum of correlated variation was observed at 39 regions; 35 of which are now described by a novel more significantly associated lead SNP, while the originally reported variant remained as the lead SNP only in 4 regions. We also confirmed two association signals in Europeans that had been previously reported only in East-Asian GWAS. Based on statistical evidence and linkage disequilibrium (LD) structure, we have curated and narrowed down the list of the most likely candidate causal variants for each region. Functional annotation using data from ENCODE filtered for PrCa cell lines and eQTL analysis demonstrated significant enrichment for overlap with bio-features within this set. By incorporating the novel risk variants identified here alongside the refined data for existing association signals, we estimate that these loci now explain ∼38.9% of the familial relative risk of PrCa, an 8.9% improvement over the previously reported GWAS tag SNPs. This suggests that a significant fraction of the heritability of PrCa may have been hidden during the discovery phase of GWAS, in particular due to the presence of multiple independent signals within the same regio

Tropospheric methane from an Amazonian floodplain lake

During July and August 1985, the sources of methane and its flux to the troposphere were measured from a houseboat laboratory anchored in Lago Calado, a stratified, dendritic lake of about 6 km2&nbsp;area, located in the central Amazon basin. Methane concentrations in the mixed layer of the lake were varied (0.0001&ndash;0.0055 mM) and usually less than 0.004 mM CH4, with no consistant temporal trend. Methane concentrations increased with depth across the thermocline as oxygen dropped to less than 0.1 mg O2&nbsp;L&minus;1. Over 6 weeks, methane increased from less than 0.08 to greater than 0.21 mM in the anoxic hypolimnion below 6 m. Methane in the pore water approached saturation, with a pure methane atmosphere within 5 cm of the sediment/water interface. The gradient-supported flux from the sediments to the overlying water could account for the methane increase in the bottom waters plus the surface flux. The measured methane flux from the surface of the open lake to the atmosphere averaged 27 mg CH4&nbsp;m&minus;2&nbsp;d&minus;1. This was consistent with the buildup in ambient methane in the nocturnal surface mixed layer of the troposphere. Ebullition contributed 70% to the average total flux. The diffusive flux measured with a static chamber ranged from 0 to 34 mg CH4&nbsp;m&minus;2&nbsp;d&minus;1, with an average of 8.3 mg m&minus;2&nbsp;d&minus;1. From July 20 to September 2, 1985, average ambient air concentration was 1.89 (&plusmn;0.16) ppm CH4, with a diurnal variation of 0.27 ppm.</span