Neurobrucellosis presenting as an intra-medullary spinal cord abscess

BACKGROUND: Of the diverse presentation of neurobrucellosis, intra-medullary spinal cord abscess is extremely rare. Only four other cases have been reported so far. We present a case of spinal cord intra-medullary abscess due to Brucella melitensis. CASE PRESENTATION: A forty-year-old female presented with progressive weakness of both lower limb with urinary incontinence of 6 months duration. She was febrile. Neurological examination revealed flaccid areflexic paraplegia with T(10 )below sensory impairment including perianal region. An intramedullary mass was diagnosed on Magnetic Resonance Image (MRI) scan extending from T(12 )to L(2). At surgery, a large abscess was encountered at the conus medullaris, from which Brucella melitensis was grown on culture. She was started on streptomycin and doxycycline for 1 month, followed by rifampicin and doxycycline for 1 month. At 2-year follow-up, she had recovered only partially and continued to have impaired bladder function. CONCLUSION: Neurobrucellosis, if not treated early, can result in severe neurological morbidity and sequale, which may be irreversible. Hence it is important to consider the possibility of neurobrucellosis in endemic region and treat aggressively

Radiological outcome of hamstring graft after anterior cruciate ligament reconstruction with augmentation of bone marrow aspirate concentrate

Background: Anterior cruciate ligament (ACL) tears are common injuries in the athletic population. Surgical intervention is required for ACL tears as it restores the knee function and it prevents early osteoarthritis. In the recent research there is increasing use of bone marrow aspirate concentrate in orthopaedics. Bone marrow aspirate concentrate (BMAC) provides mesenchymal stem cells progenitor cells and growth factors which has potential benefits in hamstring graft healing after ACL reconstruction.Methods: This was a prospective randomized case control study carried out between May 2017 and June 2020. 30 patients between age group of 18 to 40 years with complete ACL tear were divided into two groups which consisted of the control group in which only ACL reconstruction was done and second group in which ACL reconstruction was done augmenting it with bone marrow aspirate concentrate. For the BMAC group, 3 ml of BMAC was obtained in the operation theatre itself and was injected into femoral tunnel and the tibia tunnel just before portal suturing. MRI was obtained post operatively at 6 months and at 1 year. Graft healing was seen as the time taken for the graft to reach ligamentization phase when the graft became hyper intense or was visualized similar to PCL or the remnant which was preserved during reconstruction in all cases.Results: Most of the patients in which augmentation (86%) was done healed at 6 month as compared to those who were not augmented (13%).Conclusions: BMAC showed faster and better healing of the graft in ACL reconstruction

Anomaly Extraction Using Histogram-Based Detector

Now a day’s network traffic monitoring and performance of the network are more important aspect in the computer science. Anomaly Extraction is a method of detecting in large set of flow observed during an anomalous time interval, the flows associated with the one or more anomalous event. Anomaly extraction is important problem that essential for application ranging from root cause analysis and attack mitigation and anomaly extraction is also important problem for several application of testing anomaly detector. In this paper, use a meta-data provided by histogram detector for detect and identify the suspicious flow after successfully detection suspicious flow then applying the association rule mining for finding the anomalous flow. By using the rich traffic data from the meta-data of the histogram-based detector we can reduce the classification cost. In this paper, Anomaly extraction method reduce the working time which is required for analyzing alarm, its make system more practically. DOI: 10.17762/ijritcc2321-8169.15011

Implementation of Minutiae Based Fingerprint Identification System using Crossing Number Concept

Abstract: Biometric system is essentially a pattern recognition system which recognizes a person by determining the authenticity of a specific physiological (e.g., fingerprints, face, retina, iris) or behavioral (e.g., gait, signature

Percutaneous tricuspid valvotomy for pacemaker lead-induced tricuspid stenosis

AbstractPermanent pacemaker lead-induced tricuspid regurgitation is extremely uncommon. We report a patient with severe tricuspid stenosis detected 10 years after permanent single chamber pacemaker implantation in surgically corrected congenital heart disease. The loop at the level of the tricuspid valve may have caused endothelial injury and eventually led to stenosis. Percutaneous balloon valvotomy for such stenosis has not been reported from India

A randomised controlled trial to compare the efficacy of preinduction with mifepristone 12 hours versus 24 hours prior for second trimister pregnancy termination

Background: Since the second trimester termination of pregnancy is on rise due to the detection of anomalies, this study aims to provide a safe regimen with respect to efficacy, side effects and acceptability for second trimester pregnancy termination.Methods: It is a randomized controlled trial, conducted on 48 cases at BLDE Medical college, Vijayapur, Karnataka. They were divided into two groups; all patients were given mifepristone 200mg orally followed by misoprostol 400mcg vaginally after 12 hours in group I and 24 hours in group II respectively. Subsequent doses were decided depending on the Bishops score. Results were analyzed in terms of induction-abortion interval and dosage of misoprostol.Results: The mean induction abortion interval was 563.9 minutes (9.3hrs) in group I and 714.6 minutes (11.9hrs) in group II; but was statistically not significant (p value 0.611) The total dose of misoprostol used was 783.3mcg in group I compared to 550mcg in group II, but was statistically not significant. The success rate was 100% in both the groups as none of them had incomplete abortion. There were no cases of uterine rupture, infection, need for check curettage and laparotomy.Conclusions: Our study proves that the interval between mifepristone and misoprostol can be safely reduced to 12 hours without affecting the efficacy. But in gestational age <16 weeks and primigravida 24 hours interval may be of benefit. Both the regimens were 100% successful

Leptin in non PCOS and PCOS women: a comparative study

Background: Leptin, a hormone secreted by adipose tissues, controls body weight through regulation of appetite and thermogenesis.  The present study was aimed to observe role of leptin in healthy and polycystic ovary syndrome (PCOS) women.Methods: Correlation between serum leptin and anthropometric, endocrine and metabolic profile was studied in 30 apparently healthy women (control group) and 38 PCOS women (PCOS group). Each group was stratified based on body mass Index (BMI), as normal weight (BMI&lt;23) and overweight/obese (BMI&gt;23).Results Leptin level was high in 30% control group and in 65.79% PCOS group. Mean leptin (ng/ml) in PCOS group was higher compared to control group (18±1.9 v/s 12±1.7, p&lt;0.05). Mean leptin levels were higher in overweight/obese subgroup as compared to normal weight subgroup in both Control (p&lt;0.05) and PCOS groups (p=&lt;0.05). In control group, leptin showed positive correlation with waist circumference (WC) (r=-0.49, p&lt;0.01) and negative correlation with Cholesterol: HDL ratio (p&lt;0.05). In PCOS group, leptin showed positive correlation with BMI (r=0.377,p&lt;0.05) and Triglyceride (r=0.34,&lt;0.05) and negative correlation with Fasting Blood Glucose( FBG)(r=-0.33,p&lt;0.05). In normal weight subgroup among control group (n=25), leptin showed positive correlation with LDL (r=0.49, p&lt;0.05). In control overweight/obese subgroup (n=5), leptin showed positive correlation with Follicle Stimulating Hormone (FSH) (r=+1.0, p&lt;0.05) and inverse correlation with testosterone(r=-1.0,p&lt;0.05). In normal weight subgroup among PCOS group, leptin had a positive correlation with LDL: HDL ratio (r=0.488, p&lt;0.05). Conclusions: Hyperleptinemia is common in obesity. Leptin controls glycemic status in patients with IR. Correlation of leptin with FSH and testosterone is influenced by obesity and PCOS.  Leptin regulation of lipid homeostasis is influenced by obesity or PCOS

Identifikacija ptičjih vrsta lančanom reakcijom polimerazom i analizom slijeda mitohondrijskoga gena 12S rRNA

Authentic identification and differentiation of avian species is a vital step in conservative, taxonomic, forensic, legal and other ornithological interventions. The present investigation involved the application of molecular biological approach to identify and differentiate avian species i.e. two species of birds, namely black kite (Milvus migrans) and parakeet (Psittacula krameri). The DNA was isolated from blood samples of each species and a part of the mitochondrial 12S rRNA gene was amplified through polymerase chain reaction (PCR). The PCR products were sequenced and aligned using Basic Local Alignment Search Tool (BLAST) of the GenBank (NCBI). Based on the alignment and similarity/divergence, these avian species were accurately identified and differentiated.Autentična identifikacija i razlikovanje ptičjih vrsta od presudnoga su značenja u različitim konzervirajućim, taksonomskim, sudbenim, zakonskim i drugim ornitološkim aktivnostima. Ovo istraživanje bavi se molekularnobiološkim pristupom identifikaciji i razlikovanja dviju ptičjih vrsta: crvenkaste lunje (sokola) (Milvus migrans) i papige (Psittacula krameri). DNA je bila izdvojena iz uzoraka njihove krvi te je dio mitohondrijskoga 12S rRNA bio umnožen lančanom reakcijom polimerazom. Proizvodi PCR-a bili su sekvencirani i analizirani upotrebom Basic Local Alignment Search Tool (BLAST) genske banke GenBank (NCBI). Na osnovi sličnosti odnosno različitosti nalaza identificirane su te dvije pretraživane vrste

Generation, annotation, and analysis of ESTs from midgut tissue of adult female Anopheles stephensi mosquitoes

<p>Abstract</p> <p>Background</p> <p>Malaria is a tropical disease caused by protozoan parasite, <it>Plasmodium</it>, which is transmitted to humans by various species of female anopheline mosquitoes. <it>Anopheles stephensi </it>is one such major malaria vector in urban parts of the Indian subcontinent. Unlike <it>Anopheles gambiae</it>, an African malaria vector, transcriptome of <it>A. stephensi </it>midgut tissue is less explored. We have therefore carried out generation, annotation, and analysis of expressed sequence tags from sugar-fed and <it>Plasmodium yoelii </it>infected blood-fed (post 24 h) adult female <it>A. stephensi </it>midgut tissue.</p> <p>Results</p> <p>We obtained 7061 and 8306 ESTs from the sugar-fed and <it>P. yoelii </it>infected mosquito midgut tissue libraries, respectively. ESTs from the combined dataset formed 1319 contigs and 2627 singlets, totaling to 3946 unique transcripts. Putative functions were assigned to 1615 (40.9%) transcripts using BLASTX against UniProtKB database. Amongst unannotated transcripts, we identified 1513 putative novel transcripts and 818 potential untranslated regions (UTRs). Statistical comparison of annotated and unannotated ESTs from the two libraries identified 119 differentially regulated genes. Out of 3946 unique transcripts, only 1387 transcripts were mapped on the <it>A. gambiae </it>genome. These also included 189 novel transcripts, which were mapped to the unannotated regions of the genome. The EST data is available as ESTDB at <url>http://mycompdb.bioinfo-portal.cdac.in/cgi-bin/est/index.cgi</url>.</p> <p>Conclusion</p> <p>3946 unique transcripts were successfully identified from the adult female <it>A. stephensi </it>midgut tissue. These data can be used for microarray development for better understanding of vector-parasite relationship and to study differences or similarities with other malaria vectors. Mapping of putative novel transcripts from <it>A. stephensi </it>on the <it>A. gambiae </it>genome proved fruitful in identification and annotation of several genes. Failure of some novel transcripts to map on the <it>A. gambiae </it>genome indicates existence of substantial genomic dissimilarities between these two potent malaria vectors.</p