Faculty of Veterinary Medicine University of Zagreb
Abstract
Authentic identification and differentiation of avian species is a vital step in conservative, taxonomic, forensic, legal and other ornithological interventions. The present investigation involved the application of molecular biological approach to identify and differentiate avian species i.e. two species of birds, namely black kite (Milvus migrans) and parakeet (Psittacula krameri). The DNA was isolated from blood samples of each species and a part of the mitochondrial 12S rRNA gene was amplified through polymerase chain reaction (PCR). The PCR products were sequenced and aligned using Basic Local Alignment Search Tool (BLAST) of the GenBank (NCBI). Based on the alignment and similarity/divergence, these avian species were accurately identified and differentiated.Autentična identifikacija i razlikovanje ptičjih vrsta od presudnoga su značenja u različitim konzervirajućim, taksonomskim, sudbenim, zakonskim i drugim ornitološkim aktivnostima. Ovo istraživanje bavi se molekularnobiološkim pristupom identifikaciji i razlikovanja dviju ptičjih vrsta: crvenkaste lunje (sokola) (Milvus migrans) i papige (Psittacula krameri). DNA je bila izdvojena iz uzoraka njihove krvi te je dio mitohondrijskoga 12S rRNA bio umnožen lančanom reakcijom polimerazom. Proizvodi PCR-a bili su sekvencirani i analizirani upotrebom Basic Local Alignment Search Tool (BLAST) genske banke GenBank (NCBI). Na osnovi sličnosti odnosno različitosti nalaza identificirane su te dvije pretraživane vrste
