Graphene-based in-plane micro-supercapacitors with high power and energy densities

Micro-supercapacitors are important on-chip micro-power sources for miniaturized electronic devices. Although the performance of micro-supercapacitors has been significantly advanced by fabricating nanostructured materials, developing thin-film manufacture technologies and device architectures, their power or energy densities remain far from those of electrolytic capacitors or lithium thin-film batteries. Here we demonstrate graphene-based in-plane interdigital micro-supercapacitors on arbitrary substrates. The resulting micro-supercapacitors deliver an area capacitance of 80.7 μF cm(−2) and a stack capacitance of 17.9 F cm(−3). Further, they show a power density of 495 W cm(−3) that is higher than electrolytic capacitors, and an energy density of 2.5 mWh cm(−3) that is comparable to lithium thin-film batteries, in association with superior cycling stability. Such microdevices allow for operations at ultrahigh rate up to 1,000 V s(−1), three orders of magnitude higher than that of conventional supercapacitors. Micro-supercapacitors with an in-plane geometry have great promise for numerous miniaturized or flexible electronic applications

Design and development of a slot-less permanent magnet linear motor using permeance analysis method for spray application

Mostly in industrial spray applications pneumatic systems are utilized for operating the automatic spray gun. Linear motor can be one of the alternatives for triggering the spray gun instead of pneumatic systems due to its accuracy in valve positioning according to the required flow rate. From this point of view a tubular linear permanent magnet motor has been designed using Permeance Analysis Method (PAM) and developed. Three permeance models have been developed for PAM analysis. Among these three models, only one model is selected as a PAM model which can be produced the required amount of thrust for triggering the spray gun. After selecting the PAM thrust model, the size of the motor has been optimized by analyzing the effect of thrust constant, electrical and mechanical time constant. Finally based on the optimized data, the motor has been fabricated and tested that shows the good argument with the analysis result

Hepatitis E virus ORF2 protein over-expressed by baculovirus in hepatoma cells, efficiently encapsidates and transmits the viral RNA to naïve cells

A recombinant baculovirus(vBacORF2) that expressed the full-length ORF2 capsid protein of a genotype 1 strain of hepatitis E virus(HEV) was constructed. Transduction of S10-3 human hepatoma cells with this baculovirus led to large amounts of ORF2 protein production in ~50% of the cells as determined by immune fluorescence microscopy. The majority of the ORF2 protein detected by Western blot was 72 kDa, the size expected for the full-length protein. To determine if the exogenously-supplied ORF2 protein could transencapsidate viral genomes, S10-3 cell cultures that had been transfected the previous day with an HEV replicon of genotype 1 that contained the gene for green fluorescent protein(GFP), in place of that for ORF2 protein, were transduced with the vBacORF2 virus. Cell lysates were prepared 5 days later and tested for the ability to deliver the GFP gene to HepG2/C3A cells, another human hepatoma cell line. FACS analysis indicated that lysates from cell cultures receiving only the GFP replicon were incapable of introducing the replicon into the HepG2/C3A cells whereas ~2% of the HepG2/C3A cells that received lysate from cultures that had received both the replicon and the baculovirus produced GFP. Therefore, the baculovirus-expressed ORF2 protein was able to trans-encapsidate the viral replicon and form a particle that could infect naïve HepG2/C3A cells. This ex vivo RNA packaging system should be useful for studying many aspects of HEV molecular biology