6 research outputs found
AntÃgenos relevantes de Plasmodium vivax y Plasmodium falciparum detectados mediante inmunoblot : Iquitos 2004
El objetivo del presente trabajo fue identificar antÃgenos relevantes de valor diagnóstico de aislados de P. vivax y P. falciparum provenientes del departamento de Loreto, mediante la técnica de inmunoblot. Se seleccionaron pacientes entre 3 y 64 años con diagnóstico de malaria, gota gruesa positiva, procedentes de centros de salud en el departamento de Loreto. Fueron analizadas 4 mezclas de antÃgenos, una de P. falciparum (PF1) y tres de P. vivax (PV1, PV4 y PV5), preparadas a partir de 36 muestras de pacientes con alta parasitemia por P. vivax (2 700 – 69 000 parásitos/μL) y P. falciparum (2 750 – 10 000 parásitos/μL). Las mezclas de antÃgenos fueron enfrentadas a 39 sueros (12 de P. falciparum y 27 de P. vivax) mediante ensayos de inmunoblot.Tesi
A cross-sectional study of seroprevalence of strongyloidiasis in pregnant women (peruvian Amazon basin)
Strongyloidiasis is a soil-transmitted helminthiasis with a high global prevalence. Objectives:
We aimed to evaluate the prevalence of Strongyloides stercoralis infection and assess strongyloidiasis
serology as a screening technique in the Peruvian Amazon. Material and Methods: We performed a cross-sectional study of strongyloidiasis in 300 pregnant women in Iquitos (Peru) from 1 May 2019 to
15 June 2019. Women were tested using serology (Strongyloides IgG IVD-ELISA kit) as an index test
and the modified Baermann technique and/or charcoal fecal culture as the parasitological reference
standard. Results: The reference tests showed S. stercoralis in the stool of 30 women (prevalence:
10%; 95% confidence interval [CI] 7.1% to 13.9%), while 101 women tested positive on the blood
test (prevalence: 33.7%; 95% CI 28.6% to 39.4%). Fourteen of the 15 women (93.3%) with positive
results according to the modified Baermann technique, and 14 of the 23 women (56.5%) with positive
charcoal cultures also had positive serological results. Serology showed a sensitivity of 63.3% and a
negative predictive value of 94.4%. Conclusion: In Iquitos, pregnant women have a high prevalence
of S stercoralis. S. stercoralis ELISA could be an excellent tool for population-based screening, as it has
a high negative predictive value that can help to rule out the presence of active infectionThis research was co-funded by the University Development Cooperation Program, Miguel Hernández University of Elche and Generalitat Valenciana. Grant number [SOLCIF/2017/0005