The telomerase essential N-terminal domain promotes DNA synthesis by stabilizing short RNA-DNA hybrids.

Telomerase is an enzyme that adds repetitive DNA sequences to the ends of chromosomes and consists of two main subunits: the telomerase reverse transcriptase (TERT) protein and an associated telomerase RNA (TER). The telomerase essential N-terminal (TEN) domain is a conserved region of TERT proposed to mediate DNA substrate interactions. Here, we have employed single molecule telomerase binding assays to investigate the function of the TEN domain. Our results reveal telomeric DNA substrates bound to telomerase exhibit a dynamic equilibrium between two states: a docked conformation and an alternative conformation. The relative stabilities of the docked and alternative states correlate with the number of basepairs that can be formed between the DNA substrate and the RNA template, with more basepairing favoring the docked state. The docked state is further buttressed by the TEN domain and mutations within the TEN domain substantially alter the DNA substrate structural equilibrium. We propose a model in which the TEN domain stabilizes short RNA-DNA duplexes in the active site of the enzyme, promoting the docked state to augment telomerase processivity

The Effects of the Food Stamp Program on Energy Balance and Obesity

The Effects of the Food Stamp Program on Energy Balance and ObesityFood Stamp Program (FSP), Supplemental Nutrition Assistance Program (SNAP), obesity, body mass index (BMI), nutrition assistance, Food Consumption/Nutrition/Food Safety, Q18, H53, I12, I18, I38,

Polar orbit electrostatic charging of objects in shuttle wake

A survey of DMSP data has uncovered several cases where precipitating auroral electron fluxes are both sufficiently intense and energetic to charge spacecraft materials such as teflon to very large potentials in the absence of ambient ion currents. Analytical bounds are provided which show that these measured environments can cause surface potentials in excess of several hundred volts to develop on objects in the orbiter wake for particular vehicle orientations

The Effects of the Food Stamp Program on Energy Balance and Obesity

The Food Stamp Program (FSP) administered by the U.S. Department of Agriculture (USDA) is the cornerstone of the U.S. federal income and food safety net policy. The FSP has subsidized the food budget for millions of American households for over forty years, spending more than $60 billion per year in recent times. Prior research has demonstrated that women who participate in the FSP are more likely to be overweight or obese than eligible non-participants. This finding raises the concern that the additional income provided by FSP benefits induces participants to eat significantly more calories and gain weight, contributing to the U.S. obesity epidemic. Previous studies of the FSP have yielded mixed results. In this study we develop new conceptual and empirical models linking FSP participation, calorie consumption, physical activity, and weight gain, while controlling for genetic variation, weight history, and other physiological characteristics of individuals. The models enable us to test whether participants gained more weight, ate more calories, or engaged less in physical activity; or if previously omitted variables and individual health characteristics explain the higher prevalence of obesity among female FSP participants. We find a positive relationship between FSP participation and weight gain for a small subset of women. We do not find convincing evidence for the hypothesis that FSP participation causes obesity by increasing caloric consumption, decreasing physical activity, or some combination of the two. Our findings suggest that a positive association between FSP and weight exists, but we find no evidence of a direct causal link from one to the other. The association between weight and FSP likely results from confounding factors that make individuals more likely both to gain weight and to participate in the FSP.Food Stamp Program (FSP), Supplemental Nutrition Assistance Program (SNAP), obesity, body mass index (BMI), nutrition assistance, Agricultural and Food Policy, Food Consumption/Nutrition/Food Safety, Food Security and Poverty, Health Economics and Policy, Q18, H53, I12, I18, I38,

Quantifying Obesity in Economic Research: How Misleading is the Body Mass Index?

Replaced with revised version of paper 07/19/10.Obesity, percent body fat (PBF), body mass index (BMI), economic costs, measurement error, Health Economics and Policy, Research and Development/Tech Change/Emerging Technologies, C52, I10,

Angular dependence of the magnetization of isotropic superconductors: which is the vortex direction?

We present studies of the dc magnetization of thin platelike samples of the isotropic type II superconductor PbTl(10%), as a function of the angle between the normal to the sample and the applied magnetic field ${\bf H}$. We determine the magnetization vector ${\bf M}$ by measuring the components both parallel and normal to ${\bf H}$ in a SQUID magnetometer, and we further decompose it in its reversible and irreversible contributions. The behavior of the reversible magnetization is well understood in terms of minimization of the free energy taking into account geometrical effects. In the mixed state at low fields, the dominant effect is the line energy gained by shortening the vortices, thus the flux lines are almost normal to the sample surface. Due to the geometrical constrain, the irreversible magnetization ${\bf M}_{irr}$ remains locked to the sample normal over a wide range of fields and orientations, as already known. We show that in order to undestand the angle and field dependence of the modulus of ${\bf M}_{irr}$, which is a measure of the vortex pinning, and to correctly extract the field dependent critical current density, the knowledge of the modulus and orientation of the induction field ${\bf B}$ is required.Comment: 11 pages, 6 figure

On numerically accurate finite element

A general criterion for testing a mesh with topologically similar repeat units is given, and the analysis shows that only a few conventional element types and arrangements are, or can be made suitable for computations in the fully plastic range. Further, a new variational principle, which can easily and simply be incorporated into an existing finite element program, is presented. This allows accurate computations to be made even for element designs that would not normally be suitable. Numerical results are given for three plane strain problems, namely pure bending of a beam, a thick-walled tube under pressure, and a deep double edge cracked tensile specimen. The effects of various element designs and of the new variational procedure are illustrated. Elastic-plastic computation at finite strain are discussed

Urban wind power and the private sector : community benefits, social acceptance and public engagement

Given the ambitious government targets for renewable energy generation in the UK, there has been a push by government and industry towards various types and scales of Renewable Energy Technologies (RETs). This paper explores the implications of commercial urban wind projects for local communities, drawing on a case study of proposals by ASDA to construct wind turbines in two semi-urban locations in the UK. The paper argues that community responses to the proposals were complex and varied and could not adequately be encapsulated by 'nimby' (not in my back yard) assignations. It concludes that while ASDA followed a process of consulting local people, this process highlighted the problems of the 'business as usual' approach to public engagement employed by ASDA, and assumptions made about public acceptance of RETs

Validity of the Gor'kov expansion near the upper critical field in type II superconductors

We have examined the validity of the Gor'kov expansion in the strength of the order parameter of type II superconductors near the upper critical field. Although the degeneracy of the electron levels in a magnetic field gives non- perturbative terms in the solution to the Bogoliubov-de Gennes equations we find, contrary to recent claims, that these non-perturbative terms cancel in the expression for the thermodynamic potential, and that the traditional Gor'kov theory is correct sufficiently close to Hc2 at finite temperature. We have derived conditions for the validity of the Gor'kov theory which essentially state, that the change in the quasiparticle energies as compared to the normal state energies cannot be too large compared to the temperature.Comment: 5 pages, 3 figures. One reference adde

Vortex lattice for a holographic superconductor

We investigate the vortex lattice solution in a (2+1)-dimensional holographic model of superconductors constructed from a charged scalar condensate. The solution is obtained perturbatively near the second-order phase transition and is a holographic realization of the Abrikosov lattice. Below a critical value of magnetic field, the solution has a lower free energy than the normal state. Both the free energy density and the superconducting current are expressed by nonlocal functions, but they reduce to the expressions in the Ginzburg-Landau (GL) theory at long wavelength. As a result, a triangular lattice becomes the most favorable solution thermodynamically as in the GL theory of type II superconductors.Comment: v2: minor changes, references added; 11 pages, 2 figures: version to appear in PR