Novel Cold-Adapted Lipase from Marine Plankton, Salpa thompsoni

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Green and Non-Green Extractions of Eugenol from Betel Leaves Using Steam Distillation

Abstract: Eugenol is one of the most widely used natural anesthetics in the field of dentistry. It can be extracted from betel leaves with a process called steam distillation. After the steam distillation, the eugenol has to be isolated from the other oils found in the leaf, so a set of green and non-green solvents are used. The green solvent is MTBE, and the non-green solvent is dichloromethane. Both of these solvents were used in the same role which was to distill the eugenol from the remaining oils in the distillate. The research done has shown both the solvents are highly effective in the isolation of eugenol. However, the non-green solvent leads to a higher percentage of eugenol isolated from the distillation. Aside from eugenol, many other oils still remained in the final distillate as their melting points were higher than that at which the distillations occurred. Further steps can be carried out in future research to completely isolate the eugenol from the remaining oils after this entire procedure

Mrub_3018 is Orthologous to \u3cem\u3eE. coli\u3c/em\u3e B2759 (casB)

This project is part of the Meiothermus ruber genome analysis project, which uses a collection of online bioinformatics tools to predict gene function. We studied the biological activity of the Mrub_3018 gene, which we hypothesize is orthologous to E. coli gene B2759. We predicted that Mrub_3018(DNA coordinates 3057916… 3058524) encodes the protein CasB. CasB is a protein in the CRISPR CASCADE that will function as a structural protein. When the rest of the proteins form an “S” formation CasB will connect the front and back of the “S” creating a back bone for the structure. It will help bind DNA while other proteins like Cas3 degrade the protein. The hypothesis was proven by identical hits in KEGG, COG, Pfam, TIGRfam, and PDB, as well as similar location(Cytoplasm) found using PSORT-B, PRED and TMHMM. The genes were also found to be part of an operon as they were flanked by genes that are in the CRISPR system

Structure and superconductivity of LiFeAs

The lithium ions in Lithium iron arsenide phases with compositions close to LiFeAs have been located using powder neutron diffraction. These phases exhibit superconductivity at temperatures at least as high as 16 K demonstrating that superconductivity in compounds with [FeAs]- anti-PbO-type anionic layers occurs in compounds with at least three different structure types and occurs for a wide range of As-Fe-As bond angles.Comment: 3 pages, 3 figures, 3 table

Measurement of ocular compliance using iPerfusion

The pressure-volume relationship of the eye is determined by the biomechanical properties of the corneoscleral shell and is classically characterised by Friedenwald's coefficient of ocular rigidity or, alternatively, by the ocular compliance (OC), defined as dV/dP. OC is important in any situation where the volume (V) or pressure (P) of the eye is perturbed, as occurs during several physiological and pathological processes. However, accurately measuring OC is challenging, particularly in rodents. We measured OC in 24 untreated enucleated eyes from 12 C57BL/6 mice using the iPerfusion system to apply controlled pressure steps, whilst measuring the time-varying flow rate into the eye. Pressure and flow data were analysed by a “Discrete Volume” (integrating the flow trace) and “Step Response” method (fitting an analytical solution to the pressure trace). OC evaluated at 13 mmHg was similar between the two methods (Step Response, 41 [37, 46] vs. Discrete Volume, 42 [37, 48] nl/mmHg; mean [95% CI]), although the Step Response Method yielded tighter confidence bounds on individual eyes. OC was tightly correlated between contralateral eyes (R2 = 0.75, p = 0.0003). Following treatment with the cross-linking agent genipin, OC decreased by 40 [33, 47]% (p = 0.0001; N = 6, Step Response Method). Measuring OC provides a powerful tool to assess corneoscleral biomechanics in mice and other species

Salinity variance mixing in the Salish Sea is controlled by river flow

A salinity variance framework is used to study mixing in the Salish Sea, a large fjordal estuary. Output from a realistic numerical model is used to create salinity variance budgets for individual basins within the Salish Sea for 2017-2019. The salinity variance budgets quantify the mixing in each basin and diagnose the numerical mixing, which is found to contribute about one-third of the total mixing in the model. Whidbey Basin has the most intense mixing, due to its shallow depth and large river flow. Unlike in most other estuarine systems previously studied using the salinity variance method, mixing in the Salish Sea is controlled by the river flow and does not exhibit a pronounced spring-neap cycle. A mixedness\u27\u27 analysis is used to determine when mixed water is expelled from the estuary. The river flow is correlated with mixed water removal, but the coupling is not as tight as with the mixing. Because the mixing is so highly correlated with the river flow, a steady-state approximation can be used to predict the mixing in the Salish Sea and Puget Sound with good accuracy, even without any temporal averaging. Over a long time average, the mixing in Puget Sound is directly related to the exchange flow salt transport

A lightweight Web interface to Grid scheduling systems

Grid computing is often out of reach for the very scientists who need these resources because of the complexity of popular middleware suites. Some effort has gone into abstracting away these complexities using graphical user interfaces, some of which have been Web-based. This paper presents a lightweight and portable interface for Grid management, that is made possible using recent advances in dynamic technologies for Web applications. Case studies are presented to demonstrate that this interface is both usable and useful. An analysis of usage then highlights some positive and negative aspects of this approach