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Cotton, Alexander

Goodall-Copestake, Dr Will

Parker, Brenda

Parker, Dr Brenda

Roberts, Dr Iwan

Ward, Professor John

English

Engineering Conferences International

Engineering Conferences International ECI Digital Archives Microbial Engineering II Proceedings 4-3-2022 Novel Cold-Adapted Lipase from Marine Plankton, Salpa thompsoni Brenda Parker Alexander Cotton Dr Iwan Roberts Professor John Ward Dr Will Goodall-Copestake See next page for additional authors Follow this and additional works at: https://dc.engconfintl.org/microbial_ii Authors Brenda Parker, Alexander Cotton, Dr Iwan Roberts, Professor John Ward, Dr Will Goodall-Copestake, and Dr Brenda Parker NOVEL COLD-ADAPTED LIPASE FROM MARINE PLANKTON, SALPA THOMPSONI   Ekta Rayani, UCL, United Kingdom ekta.rayani.16@ucl.ac.uk Alexander Cotton, UCL, United Kingdom Dr Iwan Roberts, GE-Puridify, United Kingdom Professor John Ward, UCL, United Kingdom Dr Will Goodall-Copestake, CEH, United Kingdom Dr Brenda Parker, UCL, United Kingdom   Key Words: Salpa thompsoni, lipase, Expression, Cold-adapted, Psychrophile  Antarctica represents a rich treasure trove for novel enzymes adapted to function under extreme environmental conditions. In a search for cold adapted lipases, we mined the gut transcriptome from one of the most efficient filter feeders in Antarctic waters, the tunicate plankton Salpa thompsoni. A full-length lipase was successfully identified and synthesised into a pet28a (+) cloning vector. This gene was then heterologously expressed into the Arctic Express cell line at 10°C for 24hours; producing the lipase ‘PL002’ with a molecular weight of 66kDa. His tag affinity chromatography (Ni²⁺-NTA) was subsequently used to purify the enzyme. Characterisation of the temperature profile of PL002 lipase will contribute to our understanding of cold-tolerant versus cold-adapted enzymes. Lipases have several applications within green chemistry, and we aim to use the novel lipase from Antarctic S. thompsoni as a catalyst for transesterification reactions producing odour compounds.              Figure 1 – Salpa thompsoni with yellow-brown gut expressing lipase (picture C.Held)  

Novel Cold-Adapted Lipase from Marine Plankton, Salpa thompsoni

https://dc.engconfintl.org/cgi/viewcontent.cgi?article=1069&context=microbial_ii

Novel Cold-Adapted Lipase from Marine Plankton, Salpa thompsoni

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Engineering Conferences International