OVERDRIVE DISTORTION AND FUZZ

Distortion effects implemented using digital signal processing software MATLAB, problem description about past and current issues we deliver final product specification, implementation, evaluation of final product development

Characterization of Saccharomyces cerevisiae protein Ser/Thr phosphatase T1 and comparison to its mammalian homolog PP5

BACKGROUND: Protein Ser/Thr phosphatase 5 (PP5) and its Saccharomyces cerevisiae homolog protein phosphatase T1 (Ppt1p) each contain an N-terminal domain consisting of several tetratricopeptide repeats (TPRs) and a C-terminal catalytic domain that is related to the catalytic subunits of protein phosphatases 1 and 2A, and calcineurin. Analysis of yeast Ppt1p could provide important clues to the function of PP5 and its homologs, however it has not yet been characterized at the biochemical or cellular level. RESULTS: The specific activity of recombinant Ppt1p toward the artificial substrates (32)P-myelin basic protein (MBP) and (32)P-casein was similar to that of PP5. Dephosphorylation of (32)P-MBP, but not (32)P-casein, was stimulated by unsaturated fatty acids and by arachidoyl coenzyme A. Limited proteolysis of Ppt1p removed the TPR domain and abrogated lipid stimulation. The remaining catalytic fragment exhibited a two-fold increase in activity toward (32)P-MBP, but not (32)P-casein. Removal of the C terminus increased Ppt1p activity toward both substrates two fold, but did not prevent further stimulation of activity toward (32)P-MBP by lipid treatment. Ppt1p was localized throughout the cell including the nucleus. Levels of PPT1 mRNA and protein peaked in early log phase growth. CONCLUSIONS: Many characteristics of Ppt1p are similar to those of PP5, including stimulation of phosphatase activity with some substrates by lipids, and peak expression during periods of rapid cell growth. Unlike PP5, however, proteolytic removal of the TPR domain or C-terminal truncation only modestly increased its activity. In addition, C-terminal truncation did not prevent further activation by lipid. This suggests that these regions play only a minor role in controlling its activity compared to PP5. Ppt1p is present in both the nucleus and cytoplasm, indicating that it may function in multiple compartments. The observation that Ppt1p is most highly expressed during early log phase growth suggests that this enzyme is involved in cell growth or its expression is controlled by metabolic or nutritional signals

CHARACTERIZATION OF THERMOMECHANICAL PULP MADE FROM PINE TREES INFECTED WITH NEMATODES

Pine wilt is a lethal disease caused by the nematode Bursaphelenchus xylophilus. It causes tree death by blocking water and nutrient uptake in pine trees. Pine trees infected by these nematodes are used as fertilizer or fuel for thermal power plants, but their utilization is still only about 37%. To increase the utilization of the infected trees, this study investigated whether the shredded wood chips prepared from them could be used as raw materials for manufacturing thermomechanical pulp (TMP) and chemithermomechanical pulp (CTMP). TMP and CTMP prepared from the infected pine chips showed fewer pitch contents and better strength properties than those made from domestic pine. In conclusion, if the nematode-infected pine was used as raw materials for manufacturing TMP and CTMP, it is expected that it would contribute to the diversification of raw materials along with a high-temperature insecticidal effect. Keywords: Pine wilt, nematode disease, thermomechanical pulp, chemithermomechanical pulp, pulp strength, insecticidal effect &nbsp

Epigallocatechin-3-gallate protects toluene diisocyanate-induced airway inflammation in a murine model of asthma

AbstractEpigallocatechin-3-gallate (EGCG), a major form of tea catechin, has anti-allergic properties. To elucidate the anti-allergic mechanisms of EGCG, we investigated its regulation of matrix metalloproteinase (MMP-9) expression in toluene diisocyanate (TDI)-inhalation lung tissues as well as TNF-α and Th2 cytokine (IL-5) production in BAL fluid. Compared with untreated asthmatic mice those administrated with EGCG had significantly reduced asthmatic reaction. Also, increased reactive oxygen species (ROS) generation by TDI inhalation was diminished by administration of EGCG in BAL fluid. These results suggest that EGCG regulates inflammatory cell migration possibly by suppressing MMP-9 production and ROS generation, and indicate that EGCG may be useful as an adjuvant therapy for bronchial asthma

Effect of variation in length of the conventional heat pipe on the thermal performance

One-dimensional numerical simulations were conducted for the performance of the conventional heat pipe(CHP). In the current investigation, the operating limitations of an CHP were theoretically studied in order to determine its maximum heat transport capability. The thermal network approach was used to calculate the performance of the CHP, based on the methodology to produce results of reasonable accuracy. In the present study, the effect of variation in length of the CHP on the performance was analyzed using the corresponding thermal networks.Papers presented at the 13th International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics, Portoroz, Slovenia on 17-19 July 2017 .International centre for heat and mass transfer.American society of thermal and fluids engineers

Tribological characteristics of high strength low alloy steel under various environmental conditions

High strength low alloy steel has excellent heat resistance and high strength. As it is commonly used as gun barrel material, a long service life and superior wear resistance are necessary for steel components. Here we investigated the wear characteristics of high strength low alloy steel surfaces under various environmental conditions, using a pin-on-disk wear test. Oxidation and wear debris effects on the coefficient of friction (COF) of the alloy steel were examined under air and argon (Ar) gas flow at atmospheric conditions