9 research outputs found
Gibberellin signaling requires chromatin remodeler PICKLE to promote vegetative growth and phase transitions
© 2017 American Society of Plant Biologists. All rights reserved. PICKLE (PKL) is an ATP-dependent chromodomain-helicase-DNA-binding domain (CHD3) chromatin remodeling enzyme in Arabidopsis (Arabidopsis thaliana). Previous studies showed that PKL promotes embryonic-to-vegetative transition by inhibiting expression of seed-specific genes during seed germination. The pkl mutants display a low penetrance of the “pickle root” phenotype, with a thick and green primary root that retains embryonic characteristics. The penetrance of this pickle root phenotype in pkl is dramatically increased in gibberellin (GA)-deficient conditions. At adult stages, the pkl mutants are semidwarfs with delayed flowering time, which resemble reduced GA-signaling mutants. These findings suggest that PKL may play a positive role in regulating GA signaling. A recent biochemical analysis further showed that PKL and GA signaling repressors DELLAs antagonistically regulate hypocotyl cell elongation genes by direct protein-protein interaction. To elucidate further the role of PKL in GA signaling and plant development, we studied the genetic interaction between PKL and DELLAs using the hextuple mutant containing pkl and della pentuple (dP) mutations. Here, we show that PKL is required for most of GA-promoted developmental processes, including vegetative growth such as hypocotyl, leaf, and inflorescence stem elongation, and phase transitions such as juvenile-to-adult leaf and vegetative-to-reproductive phase. The removal of all DELLA functions (in the dP background) cannot rescue these phenotypes in pkl. RNA-sequencing analysis using the ga1 (a GA-deficient mutant), pkl, and the ga1 pkl double mutant further shows that expression of 80% of GA-responsive genes in seedlings is PKL dependent, including genes that function in cell elongation, cell division, and phase transitions. These results indicate that the CHD3 chromatin remodeler PKL is required for regulating gene expression during most of GA-regulated developmental processes
Effect of Structural Materials on Monopropellant Thruster Propulsion Performance in Micro Scale
This paper reports on the effect of structural materials on heat loss-associated propulsion performance degradation of monopropellant thrusters in the micro scale. In order to address the effect of fabrication materials on heat loss, propellant flow characteristics, and propulsion performance, a conjugate heat transfer numerical study has been conducted considering several practical substrate candidates for microthrusters. The results were analyzed with respect to the thermal diffusivity of the materials, which revealed different propulsion performance characteristics and inner nozzle flow characteristics due to varying amounts of heat loss, depending on the microfabrication materials used and propellant enthalpies. Regardless of propellant enthalpies, however, there was a dramatic degradation in the amount of the thrust produced with respect to thermal diffusivity, particularly in the range of low thermal diffusivity. Glass, among the material types compatible with fabrication processes in regard to microthrusters, exhibited a 4% degradation in thrust performance for the 50 mN class microthruster considered, with the least degradation, while copper, with 7% degradation, exhibited the greatest amount of degradation among the materials considered. With varying chamber pressure and Mach number at the nozzle exit depending on structural materials, the results also indicated the necessity of heat loss consideration in a microthruster design process
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The master growth regulator DELLA binding to histone H2A is essential for DELLA-mediated global transcription regulation
The DELLA genes, also known as 'Green Revolution' genes, encode conserved master growth regulators that control plant development in response to internal and environmental cues. Functioning as nuclear-localized transcription regulators, DELLAs modulate expression of target genes via direct protein-protein interaction of their carboxy-terminal GRAS domain with hundreds of transcription factors (TFs) and epigenetic regulators. However, the molecular mechanism of DELLA-mediated transcription reprogramming remains unclear. Here by characterizing new missense alleles of an Arabidopsis DELLA, repressor of ga1-3 (RGA), and co-immunoprecipitation assays, we show that RGA binds histone H2A via the PFYRE subdomain within its GRAS domain to form a TF-RGA-H2A complex at the target chromatin. Chromatin immunoprecipitation followed by sequencing analysis further shows that this activity is essential for RGA association with its target chromatin globally. Our results indicate that, although DELLAs are recruited to target promoters by binding to TFs via the LHR1 subdomain, DELLA-H2A interaction via the PFYRE subdomain is necessary to stabilize the TF-DELLA-H2A complex at the target chromatin. This study provides insights into the two distinct key modular functions in DELLA for its genome-wide transcription regulation in plants