2,400 research outputs found

    Synthesis of Ag Nanostructures by Photochemical Reduction Using Citrate-Capped Pt Seeds

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    A simple synthesis of Ag nanostructures such as nanorods and nanowires has been demonstrated with citrate-capped Pt seeds. UV-visible spectra and photographs of the synthesized solutions at different UV exposure times showed that the citrate-capped Pt seed played a crucial role in the growth of Ag nanostructures. After UV exposure of the colloidal solution for 60 min, the average diameter, length, and aspect ratio of the Ag nanostructures were about 95 nm, 2.1 nm, and 22, respectively. The photochemical reduction is hypothesized to result from photoelectron transfer from adsorbed citrate to Pt nanoparticle seed allowing Ag ions to form Ag nanostructures. Based on X-ray diffraction spectra and transmission electron microscope images, the synthesized Ag nanostructures were a face-centered cubic single crystal with good purity. These results suggest that the photochemical reduction method can provide Ag nanostructures in the presence of citrate-capped Pt seeds at room temperature for anisotropic Ag products

    Nicotiana benthamiana protein, NbPCIP1, interacting with Potato virus X coat protein plays a role as susceptible factor for viral infection

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    AbstractThe interactions of viral coat protein (CP) and host factors play an important role in viral replication and/or host defense mechanism. In this study, we constructed Nicotiana benthamiana cDNA library to find host factors interacting with Potato virus X (PVX) CP. Using yeast two-hybrid assay, we screened 3.3Ɨ106 independent yeast transformants from N. benthamiana cDNA library and identified six positive clones. One positive clone, named PVX CP-interacting protein 1 (NbPCIP1), is a plant-specific protein with homologue in N. tabacum (GenBank accession no. AB04049). We confirmed the PVX CPā€“NbPCIP1 interaction using yeast-two hybrid assay in yeast, proteinā€“protein binding assay in vitro, and bimolecular fluorescent complementation assay in planta. Quantitative real-time RT-PCR analysis showed that the mRNA level of NbPCIP1 increased in PVX-infected N. benthamiana plants as compared to that of healthy plants. The green fluorescent protein (sGFP)-fused NbPCIP1 (NbPCIP1-sGFP) was localized in ER or ER-associated granular-like structure of cells. When we co-express NbPCIP1-sGFP and red fluorescent protein (RFP)-fused PVX CP (PVX CP-RFP), which were introduced by transiently expressing these proteins in N. benthamiana protoplasts and epidermal cells, however, we observed the co-localization of these proteins in the inclusion body-like complex in areas surrounding nucleus. Transient over-expression and transgene silencing of NbPCIP1 assay analysis indicated that NbPCIP1 plays a critical role in viral replication during PVX infection in host plant

    Fundamental Elements for Successful Performance of CT Colonography (Virtual Colonoscopy)

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    There are many factors affecting the successful performance of CT colonography (CTC). Adequate colonic cleansing and distention, the optimal CT technique and interpretation with using the newest CTC software by a trained reader will help ensure high accuracy for lesion detection. Fecal and fluid tagging may improve the diagnostic accuracy and allow for reduced bowel preparation. Automated carbon dioxide insufflation is more efficient and may be safer for colonic distention as compared to manual room air insufflation. CT scanning should use thin collimation of ā‰¤3 mm with a reconstruction interval of ā‰¤1.5 mm and a low radiation dose. There is not any one correct method for the interpretation of CTC; therefore, readers should be well-versed with both the primary 3D and 2D reviews. Polyps detected at CTC should be measured accurately and reported following the "polyp size-based" patient management system. The time-intensive nature of CTC and the limited resources for training radiologists appear to be the major barriers for implementing CTC in Korea

    Quantum Dot-Based Light Emitting Diodes (QDLEDs): New Progress

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    In recent years, the display industry has progressed rapidly. One of the most important developments is the ability to build flexible, transparent and very thin displays by organic light emitting diode (OLED). Researchers working on this field try to improve this area more and more. It is shown that quantum dot (QD) can be helpful in this approach. In this chapter, writers try to consider all the studies performed in recent years about quantum dot-based light emitting diodes (QDLEDs) and conclude how this nanoparticle can improve performance of QDLEDs. In fact, the existence of quantum dots in QDLEDs can cause an excellent improvement in their efficiency and lifetime resulted from using improved active layer by colloidal nanocrystals. Finally, the recent progresses on the quantum dot-based light emitting diodes are reviewed in this chapter, and an important outlook into challenges ahead is prepared

    Design, manufacturing, and characterization of high-performance lightweight bipolar plates based on carbon nanotube-exfoliated graphite nanoplatelet hybrid nanocomposites

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    We report a study on manufacturing and characterization of a platform material for high-performance lightweight bipolar plates for fuel cells based on nanocomposites consisting of carbon nanotubes (CNTs) and exfoliated graphite nanoplatelets (xGnPs). The experiments were designed and performed in three steps. In the preexperimental stage, xGnP-epoxy composite samples were prepared at various xGnP weight percentages to determine the maximum processable nanofiller concentration. The main part of the experiment employed the statistics-based design of experiments (DOE) methodology to identify improved processing conditions and CNT: xGnP ratio for minimized electrical resistivity. In the postexperimental stage, optimized combinations of material and processing parameters were investigated. With the aid of a reactive diluent, 20 wt.% was determined to the be maximum processable carbon nanomaterial content in the epoxy. The DOE analyses revealed that the CNT: xGnP ratio is the most dominant factor that governs the electrical properties, and its implications in relation to CNT-xGnP interactions and microstructure are elucidated. In addition, samples fabricated near the optimized condition revealed that there exists an optimal CNT: xGnP ratio at which the electrical performance can be maximized. The electrical and mechanical properties of optimal samples suggest that CNT-xGnP hybrid nanocomposites can serve as an alternative material platform for affordable, lightweight bipolar plates.open0

    Phytosynthesis of Silver and Gold Nanoparticles Using the Hot Water Extract of Mixed Woodchip Powder and Their Antibacterial Efficacy

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    This study investigates the phytosynthesis, characterization, and antibacterial efficacy of silver and gold nanoparticles (NPs) produced using the hot water extract of mixed woodchip powder. The woodchip extract (WCE) was successfully used as both a reducing and stabilizing agent for the phytosynthesis of both crystalline metal NPs. The effects of different physicochemical factors affecting the formation of the metal NPs including reaction pH, concentration of the precursor metal salts, amount of WCE, and external energy input were evaluated. The characterization of the metal NPs was performed by transmission electron microscopy, selected area electron diffraction (SAED), energy dispersive X-ray (EDX) spectroscopy, and X-ray diffraction (XRD) pattern analysis. In addition, the antibacterial efficacy of the phytosynthesized NPs was measured. The AgNPs showed clear antibacterial activity against four representative bacterial strains. However, the AuNPs did not exhibit bactericidal activity, probably due to their surface modifications and relatively large size. These results suggest that the phytosynthesis of the metal NPs using WCE is highly efficient, and its convenience makes it suitable for use in large-scale production
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