Sintesi di nuovi composti da saggiare in vitro per l'attivita antitumorale su cellule umane di cancro pancreatico

Progettazione e sintesi di nuovi composti per valutarne l’effetto antiproliferativo e proapoptotico sulla linea cellulare di adenocarcinoma pancreatico indifferenziato MiaPaCa-2. I nuovi composti sintetizzati presentano i requisiti strutturali ritenuti importanti per l’effetto citotossico verso questa linea cellulare, infatti in essi sono presenti: una porzione ciclica planare, un raggruppamento metilenossiminico (in grado di instaurare dei legami elettrostatici con il bersaglio farmacologico), una catena laterale idrofobica (in grado di formare legami a idrogeno con il sito enzimatico grazie ai suoi sostituenti). I nuovi composti sono stati sintetizzati con lo scopo di approfondire, in particolare, lo studio della porzione ciclica planare effettuandovi modifiche tese a valutare l’importanza delle sue interazioni polari con il sito di legame: talvolta vi è stato inserito un gruppo carbonilico, possibile accettore di legame a idrogeno, talvolta non è stato inserito alcun eteroatomo in grado di instaurare legami a idrogeno ma la porzione ciclica planare è stata sostituita con un nucleo diidronaftalenico per aumentarne la lipofilicità. Synthesis of new compounds to test in vitro on human undifferentiated pancreatic cancer cells MIA PaCa-2, which express Fibroblast Growth Factor Receptor (FGFR),for their potential antitumoral activity

ICS Materials. Towards a re-Interpretation of material qualities through interactive, connected, and smart materials.

The domain of materials for design is changing under the influence of an increased technological advancement, miniaturization and democratization. Materials are becoming connected, augmented, computational, interactive, active, responsive, and dynamic. These are ICS Materials, an acronym that stands for Interactive, Connected and Smart. While labs around the world are experimenting with these new materials, there is the need to reflect on their potentials and impact on design. This paper is a first step in this direction: to interpret and describe the qualities of ICS materials, considering their experiential pattern, their expressive sensorial dimension, and their aesthetic of interaction. Through case studies, we analyse and classify these emerging ICS Materials and identified common characteristics, and challenges, e.g. the ability to change over time or their programmability by the designers and users. On that basis, we argue there is the need to reframe and redesign existing models to describe ICS materials, making their qualities emerge

Anderson transition on the Bethe lattice: an approach with real energies

We study the Anderson model on the Bethe lattice by working directly with propagators at real energies $E$. We introduce a novel criterion for the localization-delocalization transition based on the stability of the population of the propagators, and show that it is consistent with the one obtained through the study of the imaginary part of the self-energy. We present an accurate numerical estimate of the transition point, as well as a concise proof of the asymptotic formula for the critical disorder on lattices of large connectivity, as given in [P.W. Anderson 1958]. We discuss how the forward approximation used in analytic treatments of localization problems fits into this scenario and how one can interpolate between it and the correct asymptotic analysis.Comment: Close to published versio

Examination of Indoor Mobile Mapping Systems in a Diversified Internal/External Test Field

Nowadays, simultaneous localization and mapping (SLAM) algorithms support several commercial sensors which have recently been introduced to the market, and, like the more common mobile mapping systems (MMSs), are designed to acquire three-dimensional and high-resolution point clouds. The new systems are said to work both in external and internal environments, and completely avoid the use of targets and control points. The possibility of increasing productivity in three-dimensional digitization projects is fascinating, but data quality needs to be carefully evaluated to define appropriate fields of application. The paper presents the analytical measurement principle of these indoor mobile mapping systems (IMMSs) and the results of some tests performed on three commercial systems. A common test field was defined in order to acquire comparable data. By taking the already available terrestrial laser scan survey as the ground truth, the datasets under examination were compared with the reference and some assessments are presented which consider both quantitative and qualitative aspects. Geometric deformation in the final models was computed using the so-called Multiscale Model to Model Cloud Comparison (M3C2) algorithm. Cross sections and cloud to mesh (C2M) distances were also employed for a more detailed analysis. The real usability assessment is based on the features of recognizability, double surface evidence, and visualization effectiveness. For these evaluations, comparative images and tables are presented. © 2018 by the authors

A cohort study on acute ocular motility disorders in pediatric emergency department

Background: Acute ocular motility disorders (OMDs) in children admitted to Emergency Department (ED) represents a not so rare condition with a wide spectrum of different etiologies. The emergency physician must be skilled in rapidly identifying patients with potentially life threatening (LT) forms, requiring further diagnostic procedures. The aim of the study was to assess characteristics of children with acute Ocular Motility Disorders (OMDs), and to identify "red flags" for recognition of underlying life-threatening (LT) conditions. Methods: A retrospective cohort study evaluated children (2 months-17 years) admitted to a tertiary Emergency Department in 2009-2014. A subgroup analysis was performed comparing children with and without LT conditions. Results: Of 192 visits for OMDs, the isolated strabismus occurred most frequently (55.6%), followed by pupil disorders (31.8%), ptosis (5.2%) and combined OMDs (11.5%). The majority of acute OMDs involved no underlying LT conditions (n = 136) and most of them were infants or toddlers (50%). In a multivariable analysis, LT conditions included especially children over 6 years of age, increasing the odds ratio by 2% for each months of age (p = 0.009). LT etiologies were 16 times more likely in combined OMDs (p = 0.018), were over 13 times more likely to report associated extra-ocular signs/symptoms (p = 0.017) and over 50 times more likely to report co-morbidity (p = 0.017). Conclusion: OMDs are not an uncommon presentation at ED. Although most of them involve non-LT conditions, the ED physician should consider potential "red flags" for appropriate management of children such as age > 6 years, combined OMDs, extra-ocular symptoms and co-morbidity

Chapter Metodi innovativi per l’insegnamento della Geomatica: il video a 360° come strumenti per le esercitazioni sui sistemi a scansione 3D

The chapter illustrates the results of the educational use of 360° video in the field of Geomatics. Geomatics is the discipline that deals with the collection, analysis, and interpretation of spatial data with the contribution of information technologies. The GeCo Lab has been experimenting in the last few years innovative educational approaches supported by multimedia and interactive tools. The goal within the SEPA360 project has been to use the potentialities of 360° videos to simulate surveying activities, by using topography and laser scanning systems, for the documentation of built heritage, to provide teaching supporting materials for different Geomatic courses active at the University of Florence. The final outputs consist of two videos: one non-interactive with an “educational” approach, to introduce some theoretical concepts, and the other one with an “self-evaluation” purpose, with interactions, added in Vivista

Chemical Profiling of Astragalus membranaceus Roots (Fish.) Bunge Herbal Preparation and Evaluation of Its Bioactivity

Astragalus membranaceus (Fish.) Bunge is a perennial herb distributed in the northern part of China, and its roots, namely, Hang qi, are included as a natural ingredient in dietary supplement formulations commonly used to treat different disorders such as respiratory infections, diabetes, and heart failure. The availability of a simple method for the determination of the quality of Astragalus herbal preparations could be a challenging issue for commercial purposes. In this study, a liquid chromatography–mass spectrometry (LC–MS)/MS based approach was used to characterize specialized metabolite recovery of 3 commercial hydroalcoholic extracts of A. membranaceus (AMG1, AMG2, AMG3) in addition to a hydroalcoholic extract of A. membranaceus root (AST). The hypoglycemic effect, cholinesterase inhibition, and antioxidant activities were also evaluated. Thirty-one compounds, of which 19 polyphenols and 12 saponins, were identified. The extracts were also quantified by using a sensitive and selective Q-Trap system for their content in flavonoids and astragalosides, selecting astragaloside I and IV as chemical markers. From our results, AMG3 preparation (Axtragyl) was the most abundant in terms of both specialized classes of metabolites, showing a fingerprint similar to that of AST. Interestingly, tested enzyme inhibition ability of flavonoids, daidzein (11) and formononetin (19), reported a higher α-glucosidase inhibition in comparison with that of acarbose used as positive control. The in silico study clarified the interactions among the molecules and the importance of having a free hydroxy group. Moreover, Axtragyl was able to exert protective effects in Caco-2 cells treated with hydrogen peroxide, confirming its ability as a potential protective agent in intestinal injury

Effects of exercise training on cardiovascular adrenergic system

In heart failure (HF), exercise has been shown to modulate cardiac sympathetic hyperactivation which is one of the earliest features of neurohormonal derangement in this syndrome and correlates with adverse outcome. An important molecular alteration related to chronic sympathetic overstimulation in HF is represented by cardiac β-adrenergic receptor (β-AR) dysfunction. It has been demonstrated that exercise reverses β-AR dysfunction by restoring cardiac receptor membrane density and G-protein-dependent adenylyl cyclase activation. In particular, several evidence indicate that exercise reduces levels of cardiac G-protein coupled receptor kinase-2 (GRK2) which is known to be involved in both β1-AR and β2-AR dysregulation in HF. Similar alterations of β-AR system have been described also in the senescent heart. It has also been demonstrated that exercise training restores adrenal GRK2/α-2AR/catecholamine (CA) production axis. At vascular level, exercise shows a therapeutic effect on age-related impairment of vascular reactivity to adrenergic stimulation and restores β-AR-dependent vasodilatation by increasing vascular β-AR responsiveness and reducing endothelial GRK2 activity. Sympathetic nervous system overdrive is thought to account for >50% of all cases of hypertension and a lack of balance between parasympathetic and sympathetic modulation has been observed in hypertensive subjects. Non-pharmacological, lifestyle interventions have been associated with reductions in SNS overactivity and blood pressure in hypertension. Several evidence have highlighted the blood pressure lowering effects of aerobic endurance exercise in patients with hypertension and the significant reduction in sympathetic neural activity has been reported as one of the main mechanisms explaining the favorable effects of exercise on blood pressure control