HISTOCHEMICAL DISTRIBUTION OF LEUCINE AMINOPEPTIDASE (LAP-ase)IN THE YOUNG RABBIT INTESTINE

[EN] The distribution of leucine aminopeptidase in normal duodenal, jejunal, ileal, crecal and colonic mucosa of the rabbit has been studied using a histochemical method. Thirty New Zealand White rabbits were used ranging from 26-day old foetuses to 43-day old young (3 rabbits per age). Small intestine siles performed a leucine aminopeptidase reaction starting from the 301 h day of foetal life. After birth the reaction was variable in intensity up to 19 days of age, while in older animals it became very strong. Similarly, the large intestine followed the same pattern with the exception that from the 241 h day of age onwards it performed no reaction.[FR] La méthode histochimique a été utilisée pour étudier la répartition de la leucine aminopeptidase dans les muqueuses du duodénum, du jéjunum, de l'iléon, du caecum et du colon de jeunes lapins. Trente lapins néo-zélandais blancs allant de foetus ilgés de 26 jours jusqu'a de jeunes lapereaux ilgés de 43 jours ont été utilisés (3 par stade d'observation). La leucine aminopeptidase a été détectée dans l'intestin gréle a partir du 30éme jour de vie foetale. Aprés la naissance l'intensité de la réponse est variable jusqu'a 19 jours d'ilge, tandis qu'elle devient trés forte chez les animaux plus ilgés. Le gros intestin suit le méme modéle avec une exception notable : a partir de l'ilge de 24 jours il n'y a plus d'activité détectable.Sabatakou, O.; Xylouri-Frangiadaki, E.; Paraskevakou, E. (2000). HISTOCHEMICAL DISTRIBUTION OF LEUCINE AMINOPEPTIDASE (LAP-ase)IN THE YOUNG RABBIT INTESTINE. World Rabbit Science. doi:10.4995/wrs.2000.434SWORD08

HISTOCHEMICAL STUDY OF NON-SPECIFIC ESTERASE ACTIVITY IN SMALL AND LARGE INTESTINE OF YOUNG RABBIT

[EN] The distribution of non-specific esterase in normal duodenal, jejunal, ileal and large intestinal mucosa of the rabbit has been studied using histochemical methods. Thirty three New Zealand White rabbits were used ranging from 26-day old fetuses to 43-day old young (3 rabbits I age). Distribution and intensity (strong to very strong) varied little throughout the small and large intestine from the 301 h day of foetal life and as after birth up to 43 days. At the 26 or 281 h days of foetal life only mild to positive reactions were seen. 1 In the caecum and in the upper colon, a low non-specific esterase activity was observed in the 7- and 15 day-old rabbits, although this activity was very strong in younger one-day-old rabbits, and in the 19-day-old and older rabbits.[FR] La répartition de l'estérase non spécifique dans la muqueuse du duodénum, du jéjunum, de l'ileum et du gros intestin du lapin a été étudiée en utilisant une méthode histochimique. Trente trois lapins néozélandais blancs allant de fcetus é'.!gés de 26 jours jusqu'a de jeunes lapereaux é'.!gés de 43 jours ont été utilisés (3 lapins a chaque é'.!ge). La répartition et l'intensité de l'activité (forte a tres forte) varient peu dans le gros intestin et le gréle a partir du 3o•m• jour de vie fcetale et jusqu'au 43éme jour. Par contre, au 26-2a•m• jour de vie fcetale il y a une réaction seulement positive ou moyenne. D'autre part, il faut souligner que dans le caecum et le colon (partie haute) un faible activité de l'estérase non spécifique est observée chez les lapereaux é'.!gés de 7 et 15 jours alors que l'activité est tres forte chez les lapereaux d'1 jour et chez ceux de 19 jours et plus.Sabatakou, O.; Xylouri-Frangiadaki, E.; Paraskevakou, E. (2000). HISTOCHEMICAL STUDY OF NON-SPECIFIC ESTERASE ACTIVITY IN SMALL AND LARGE INTESTINE OF YOUNG RABBIT. World Rabbit Science. doi:10.4995/wrs.2000.435SWORD08

Hemolytic-uremic syndrome, malignant hypertension and IgA nephropathy: Successful treatment with plasma exchange therapy

A young patient with hemolytic-uremic syndrome and malignant hypertension with serious deterioration of renal function is described whose biopsy specimen showed additional IgA mesangial deposits. The patient responded to steroid treatment and to plasma exchange therapy without the need of hemodialysis sessions. In the following years, he achieved clinical remission and his blood pressure was in normal ranges without any further complications. IgA glomerulonephritis is rarely associated to hemolytic-uremic syndrome and malignant hypertension, with only a few previously described cases. We present an overview of potential pathophysiological connections between these diseases. © 2012 Elsevier Ltd

Epidermolysis bullosa acquisita: treatment with intravenous immunoglobulins

Epidermolysis bullosa acquisita (EBA) is a rare autoimmune bullous disorder that is often difficult to treat. Few cases have been reported and therapy consists mainly of combinations of systemic steroids, immunosuppressants and, recently, administration of intravenous human immunoglobulin (IVIg). We describe a case of EBA in which our therapeutic choices were limited due to the patient’s poor general condition, including extensive infection of the lesions and a history of pulmonary tuberculosis. The patient was treated with IVIg at a dose of 400 mg/kg per day for 5 consecutive days every 4 weeks. The treatment was wen tolerated and the results were satisfactory. It seems that IVIg, due to its possible immunomodulatory mode of action, can be an efficacious therapeutic agent in this rare autoimmune disease

Association between microvessel density and histologic grade in renal cell carcinomas

Angiogenesis seems to contribute to tumor growth and the development of metastases. There may be an association between the vascular density of individual tumors and their prognosis. In the present survey we studied 53 cases of renal cell carcinoma investigating possible relationship between histologic grade and microvessel density (MVD) measured by an image analysis system. According to our results MVD was significantly associated with the histologic grade, higher grades being accompanied with a higher MVD. Further studies are needed to investigate a possible connection of MVD with the prognostic role of grade in RCCs. © 2007 Arányi Lajos Foundation

Immunohistochemical evaluation of podocalyxin expression in glomerulopathies associated with nephrotic syndrome

It is now well established that morphological change of podocytes is closely correlated to the development of proteinuria. The aim of this study was to investigate the role of podocalyxin, a major podocyte protein, in the pathogenesis of glomerulopathies primarily associated with the nephrotic syndrome. Immunohistochemical expression of podocalyxin has been evaluated in 51 renal samples, including healthy controls, patients with podocytopathies (minimal change disease [MCD], focal segmental glomerulosclerosis [FSGS]) and membranous glomerulopathy (MG). A computerized image analysis program has been used. Statistical analysis was performed using analysis of variance and Bonferroni tests. Immunohistochemical expression of podocalyxin has been observed within the podocytes of healthy controls. In MCD, podocalyxin expression was globally reduced despite the normal appearance of the glomeruli. In FSGS, podocalyxin loss was observed in both the segmental sclerotic and the nonsclerotic areas being significantly more prominent in the former. Reduction of podocalyxin in MG was demonstrated for the first time immunohistochemically. The percentage of the stained area was statistical significantly higher in the controls than in each pathologic group. However, among pathologic groups (FSGS, MCD, MG), there was no statistically significant difference. This is one of the few studies investigating podocalyxin immunohistochemical expression in glomerulopathies associated with nephrotic syndrome. The observed reduction in podocalyxin expression suggests that it constitutes a target molecule in nephrotic syndrome pathogenesis regardless of the underlying cause. © 2011 Elsevier Inc. All rights reserved

Membranoproliferative glomerulonephritis in the setting of multicentric angiofollicular lymph node hyperplasia (Castleman's disease) complicated by Evan's syndrome

Systemic Castleman's disease is a lymphoproliferative disorder with various clinical presentations and incompletely understood aetiology. The authors report on a rare case of the plasma cell variant of Castleman's disease associated with autoimmune haemolytic anaemia and autoimmune thrombocytopenia (Evan's syndrome) and complicated by mixed nephrotic - nephritic syndrome and acute renal failure due to an underlying glomerulopathy with microscopic and immunofluorescence findings suggestive of membranoproliferative glomerulonephritis (MPGN) type I. Immunocomplexed glomerulonephritis is rare in Castleman's disease, while, to the best of our knowledge, constellation of all these autoimmune phenomena is reported for the first time suggesting that apart from the putative role of VEGF and IL-6 in the pathogenesis of the disease, a more generalised immunological disturbance occurs, probably through autoantibodies induced by active polyclonal B cells raised from Castleman's disease tumour