7 research outputs found
Prevalence of periodontal disease in dogs and owners' level of awareness - a prospective clinical trial
Differential Seroprevalence of Human Bocavirus Species 1-4 in Beijing, China
BACKGROUND: Four species of human bocaviruses (HBoV1-4) have been identified based on phylogenetic analysis since its first report in 2005. HBoV1 has been associated with respiratory disease, whereas HBoV2-4 are mainly detected in enteric infections. Although the prevalence of HBoVs in humans has been studied in some regions, it has not been well addressed globally. METHODOLOGY/PRINCIPAL FINDINGS: Cross-reactivity of anti-VP2 antibodies was detected between HBoV1, 2, 3, and 4 in mouse and human serum. The prevalence of specific anti-VP2 IgG antibodies against HBoV1-4 was determined in different age groups of healthy individuals aged 0-70 years old in Beijing, China, using a competition ELISA assay based on virus-like particles of HBoV1-4. The seroprevalence of HBoV1-4 was 50%, 36.9%, 28.7%, and 0.8%, respectively, in children aged 0-14 years (n = 244); whereas the seroprevalence of HBoV1-4 was 66.9%, 49.3%, 38.7% and 1.4%, respectively, in healthy adults (≥ 15 years old; n = 142). The seropositive rate of HBoV1 was higher than that of HBoV2, HBoV3, and HBoV4 in individuals older than 0.5 years. Furthermore, IgG seroconversion of HBoV1 (10/31, 32.3%), HBoV2 (8/31, 25.8%), and HBoV3 (2/31, 6.5%) was found in paired sera collected from children with respiratory tract infections who were positive for HBoV1 according to PCR analysis. CONCLUSIONS/SIGNIFICANCE: Our data indicate that HBoV1 is more prevalent than HBoV2, HBoV3, and HBoV4 in the population we sampled in Beijing, China, suggesting that HBoV species may play differential roles in disease
Estimativa de populações canina e felina domiciliadas em zona urbana do Estado de São Paulo Estimate of the owned canine and feline populations in urban area in Brazil
OBJETIVO: Dada a importância do conhecimento acerca da população de cĂŁes e gatos domiciliados para o adequado planejamento e avaliação das ações de controle da raiva em áreas urbanas, propõe-se a utilização de um valor preciso de estimativa dessas populações com base em indicadores populacionais humanos. MÉTODOS: Foi calculada a razĂŁo entre as populações humana e animal (canina e felina) por meio de inspeções domiciliares, no MunicĂpio de TaboĂŁo da Serra, SP. O municĂpio foi dividido em duas áreas homogĂŞneas distintas socioeconomicamente, de acordo com o algoritmo da mĂ©dia k, de modo a permitir a comparação das razões homem:animal (cĂŁo e gato) das áreas homogĂŞneas. RESULTADOS: A razĂŁo entre a população humana e a população canina foi 5,14 e a entre a humana e a felina foi 30,57. NĂŁo foi observada diferença significativa ao comparar-se as razões entre as populações humana e animal das áreas homogĂŞneas. CONCLUSĂ•ES: A adoção de uma metodologia de estimativa populacional canina e felina domiciliada, baseada em indicadores populacionais humanos, Ă© a mais indicada e facilmente exeqĂĽĂvel quando comparada ao censo canino.<br>OBJECTIVE: Given the importance of assessing owned dog and cat populations to adequate planning and evaluation of rabies control measures in urban areas, it is proposed the use of an estimate of these populations based on human population parameters. METHODS: The ratio between human population and owned animal (dogs and cats) population was calculated in the municipality of TaboĂŁo da Serra, state of SĂŁo Paulo, Brazil. This municipality was divided into two distinct social and economic homogeneous areas through k-mean algorithm, allowing for comparison between the ratios of the two homogeneous areas. RESULTS: A 5.14 ratio was calculated for human and dog populations and a 30.57ratio for human and feline populations. A significant difference was not observed when comparing the ratios for human and animal populations of the two homogeneous areas. CONCLUSIONS: An estimate of owned dog and cat populations based on the human population is the better choice for assessing the animal population instead of an animal census because it can be easily implemented
Release-Recapture Test of Dispersal and Survival of Sterile Males of Ceratitis capitata (Diptera: Tephritidae)
Trapping to monitor tephritid movement: Results, best practice, and assessment of alternatives
Movement of tephritid flies underpins their survival, reproduction, and ability to establish in new areas and is thus of importance when designing effective management strategies. Much of the knowledge currently available on tephritid movement throughout landscapes comes from the use of direct or indirect methods that rely on the trapping of individuals. Here, we review published experimental designs and methods from mark-release-recapture (MRR) studies, as well as other methods, that have been used to estimate movement of the four major tephritid pest genera (Bactrocera, Ceratitis, Anastrepha, and Rhagoletis). In doing so, we aim to illustrate the theoretical and practical considerations needed to study tephritid movement. MRR studies make use of traps to directly estimate the distance that tephritid species can move within a generation and to evaluate the ecological and physiological factors that influence dispersal patterns. MRR studies, however, require careful planning to ensure that the results obtained are not biased by the methods employed, including marking methods, trap properties, trap spacing, and spatial extent of the trapping array. Despite these obstacles, MRR remains a powerful tool for determining tephritid movement, with data particularly required for understudied species that affect developing countries. To ensure that future MRR studies are successful, we suggest that site selection be carefully considered and sufficient resources be allocated to achieve optimal spacing and placement of traps in line with the stated aims of each study. An alternative to MRR is to make use of indirect methods for determining movement, or more correctly, gene flow, which have become widely available with the development of molecular tools. Key to these methods is the trapping and sequencing of a suitable number of individuals to represent the genetic diversity of the sampled population and investigate population structuring using nuclear genomic markers or non-recombinant mitochondrial DNA markers. Microsatellites are currently the preferred marker for detecting recent population displacement and provide genetic information that may be used in assignment tests for the direct determination of contemporary movement. Neither MRR nor molecular methods, however, are able to monitor fine-scale movements of individual flies. Recent developments in the miniaturization of electronics offer the tantalising possibility to track individual movements of insects using harmonic radar. Computer vision and radio frequency identification tags may also permit the tracking of fine-scale movements by tephritid flies by automated resampling, although these methods come with the same problems as traditional traps used in MRR studies. Although all methods described in this chapter have limitations, a better understanding of tephritid movement far outweighs the drawbacks of the individual methods because of the need for this information to manage tephritid populations