Glued versus stapled anastomosis of the colon: An experimental study to determine comparative resistance to intraluminal pressure

This study compared the resistance of glued versus stapled anastomosis of the colon to intraluminal pressures at different times during healing. Forty seven female domestic pigs, mean weight of 30.7 kg, were used. Maximum physiological luminal pressures, i.e. the pressure resisted by a catheter inserted into the intestinal lumen via a puncture without it being released and without injury to the surrounding intestinal wall, was performed in 5 control animals. The remaining 42 animals were divided into 3 groups of 14 animals each based on time from anastomosis construction. Each group was divided into 2 subgroups with stapled or glued anastomoses. Intraluminal pressure was measured on the first, third, and fifth day post-surgery. The maximum pressures resisted by anastomoses were significantly higher than the physiological pressures in all groups. At all time points, stapled anastomoses resisted higher intraluminal pressures than glued ones. However, glued anastomoses resisted pressures significantly higher than physiological pressure. As healing advanced, glued anastomoses neared the resistance to intraluminal pressures of stapled anastomoses. Healing with absorbable synthetic glue was as good as with staples. Glued anastomoses resisted pressures that were statistically significantly higher than physiological intraluminal colon pressures but lower than stapled ones

Glued versus stapled anastomosis of the colon: An experimental study to determine comparative resistance to intraluminal pressure

Background: This study compared the resistance of glued versus stapled anastomosis of the colon to intraluminal pressures at different times during healing. Methods: Forty seven female domestic pigs, mean weight of 30.7 kg, were used. Maximum physiological luminal pressures, i.e. the pressure resisted by a catheter inserted into the intestinal lumen via a puncture without it being released and without injury to the surrounding intestinal wall, was performed in 5 control animals. The remaining 42 animals were divided into 3 groups of 14 animals each based on time from anastomosis construction. Each group was divided into 2 subgroups with stapled or glued anastomoses. Intraluminal pressure was measured on the first, third, and fifth day post-surgery. Results: The maximum pressures resisted by anastomoses were significantly higher than the physiological pressures in all groups. At all time points, stapled anastomoses resisted higher intraluminal pressures than glued ones. However, glued anastomoses resisted pressures significantly higher than physiological pressure. As healing advanced, glued anastomoses neared the resistance to intraluminal pressures of stapled anastomoses. Conclusion: Healing with absorbable synthetic glue was as good as with staples. Glued anastomoses resisted pressures that were statistically significantly higher than physiological intraluminal colon pressures but lower than stapled ones

Pharmacokinetics of intramuscularly administered thermoresponsive polymers

Aqueous solutions of some polymers exhibit a lower critical solution temperature (LCST); that is, they form phase-separated aggregates when heated above a threshold temperature. Such polymers found many promising (bio)medical applications, including in situ thermogelling with controlled drug release, polymer-supported radiotherapy (brachytherapy), immunotherapy, and wound dressing, among others. Yet, despite the extensive research on medicinal applications of thermoresponsive polymers, their biodistribution and fate after administration remained unknown. Thus, herein, they studied the pharmacokinetics of four different thermoresponsive polyacrylamides after intramuscular administration in mice. In vivo, these thermoresponsive polymers formed depots that subsequently dissolved with a two-phase kinetics (depot maturation, slow redissolution) with half-lives 2 weeks to 5 months, as depot vitrification prolonged their half-lives. Additionally, the decrease of T-CP of a polymer solution increased the density of the intramuscular depot. Moreover, they detected secondary polymer depots in the kidneys and liver; these secondary depots also followed two-phase kinetics (depot maturation and slow dissolution), with half-lives 8 to 38 days (kidneys) and 15 to 22 days (liver). Overall, these findings may be used to tailor the properties of thermoresponsive polymers to meet the demands of their medicinal applications. Their methods may become a benchmark for future studies of polymer biodistribution