19 research outputs found
Circulating small RNA signatures differentiate accurately the subtypes of muscular dystrophies: small-RNA next-generation sequencing analytics and functional insights
Muscular dystrophies are a group of rare and severe inherited disorders mainly affecting the muscle tissue. Duchene Muscular Dystrophy, Myotonic Dystrophy types 1 and 2, Limb Girdle Muscular Dystrophy and Facioscapulohumeral Muscular Dystrophy are some of the members of this family of disorders. In addition to the current diagnostic tools, there is an increasing interest for the development of novel non-invasive biomarkers for the diagnosis and monitoring of these diseases. miRNAs are small RNA molecules characterized by high stability in blood thus making them ideal biomarker candidates for various diseases. In this study, we present the first genome-wide next-generation small RNA sequencing in serum samples of five different types of muscular dystrophy patients and healthy individuals. We identified many small RNAs including miRNAs, lncRNAs, tRNAs, snoRNAs and snRNAs, that differentially discriminate the muscular dystrophy patients from the healthy individuals. Further analysis of the identified miRNAs showed that some miRNAs can distinguish the muscular dystrophy patients from controls and other miRNAs are specific to the type of muscular dystrophy. Bioinformatics analysis of the target genes for the most significant miRNAs and the biological role of these genes revealed different pathways that the dysregulated miRNAs are involved in each type of muscular dystrophy investigated. In conclusion, this study shows unique signatures of small RNAs circulating in five types of muscular dystrophy patients and provides a useful resource for future studies for the development of miRNA biomarkers in muscular dystrophies and for their involvement in the pathogenesis of the disorders
A score for predicting acute kidney injury following coronary artery bypass graft surgery in an Asian population
10.1053/j.jvca.2016.03.135Journal of Cardiothoracic and Vascular Anesthesia3051296-130
The hepatoprotective effect of hepatic stimulator substance (HSS) against liver regeneration arrest induced by acute ethanol intoxication
Male Wistar rats were randomized to receive ethanol (2.5 ml/kg by
gastric intubation every 8 hr; group I), equal volumes of isocaloric to
ethanol sucrose solution (group II), or ethanol and HSS (100 mg/kg
intraperitoneally 10 and 16 hr after partial hepatectomy; groups III and
IV, respectively) for up to 96 hr after partial hepatectomy, with
ethanol administration starting 1 hr prior to partial hepatectomy.
Animals were killed at 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, 48, 60,
and 96 hr after partial hepatectomy. The rate of liver regeneration was
evaluated by the mitotic index in H&E-stained sections, immunochemical
detection of Ki67 nuclear antigen, rate of [H-3]thymidine
incorporation into hepatic DNA, and liver thymidine kinase enzymatic
activity. The biological activity of HSS in groups I and II rats was
evaluated using a bioassay. Ethanol administration arrested liver
regeneration during the first 32 hr after partial hepatectomy and
suppressed HSS activity throughout the period examined. Liver
regeneration progressed after 32 hr despite the low levels of HSS
activity. HSS administration at 10 and 16 hr reversed liver regeneration
arrest induced by ethanol. Acute ethanol administration induces cell
cycle arrest during the first 32 hr after partial hepatectomy and
suppression of HSS biological activity seems to contribute to this
effect. HSS administration reversed the inhibitory effect of ethanol on
liver regeneration and caused synchronized entrance of hepatocytes in
the S phase of the cell cycle. HSS seems to participate in the network
of growth factors controlling the G1/S cell cycle checkpoint
Circulating small RNA signatures differentiate accurately the subtypes of muscular dystrophies: small-RNA next-generation sequencing analytics and functional insights
Muscular dystrophies are a group of rare and severe inherited disorders mainly affecting the muscle tissue. Duchene Muscular Dystrophy, Myotonic Dystrophy types 1 and 2, Limb Girdle Muscular Dystrophy and Facioscapulohumeral Muscular Dystrophy are some of the members of this family of disorders. In addition to the current diagnostic tools, there is an increasing interest for the development of novel non-invasive biomarkers for the diagnosis and monitoring of these diseases. miRNAs are small RNA molecules characterized by high stability in blood thus making them ideal biomarker candidates for various diseases. In this study, we present the first genome-wide next-generation small RNA sequencing in serum samples of five different types of muscular dystrophy patients and healthy individuals. We identified many small RNAs including miRNAs, lncRNAs, tRNAs, snoRNAs and snRNAs, that differentially discriminate the muscular dystrophy patients from the healthy individuals. Further analysis of the identified miRNAs showed that some miRNAs can distinguish the muscular dystrophy patients from controls and other miRNAs are specific to the type of muscular dystrophy. Bioinformatics analysis of the target genes for the most significant miRNAs and the biological role of these genes revealed different pathways that the dysregulated miRNAs are involved in each type of muscular dystrophy investigated. In conclusion, this study shows unique signatures of small RNAs circulating in five types of muscular dystrophy patients and provides a useful resource for future studies for the development of miRNA biomarkers in muscular dystrophies and for their involvement in the pathogenesis of the disorders